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DNA Methylation of POMC and NR3C1-1F and Its Implication in Major Depressive Disorder and Electroconvulsive Therapy
Introduction Precision medicine in psychiatry is still in its infancy. To establish patient-tailored treatment, adequate indicators predicting treatment response are required. Electroconvulsive therapy (ECT) is considered one of the most effective options for pharmacoresistant major depressive disor...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Georg Thieme Verlag KG
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070046/ https://www.ncbi.nlm.nih.gov/pubmed/36944329 http://dx.doi.org/10.1055/a-2034-6536 |
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author | Maier, Hannah B. Moschny, Nicole Eberle, Franziska Jahn, Kirsten Folsche, Thorsten Schülke, Rasmus Bleich, Stefan Frieling, Helge Neyazi, Alexandra |
author_facet | Maier, Hannah B. Moschny, Nicole Eberle, Franziska Jahn, Kirsten Folsche, Thorsten Schülke, Rasmus Bleich, Stefan Frieling, Helge Neyazi, Alexandra |
author_sort | Maier, Hannah B. |
collection | PubMed |
description | Introduction Precision medicine in psychiatry is still in its infancy. To establish patient-tailored treatment, adequate indicators predicting treatment response are required. Electroconvulsive therapy (ECT) is considered one of the most effective options for pharmacoresistant major depressive disorder (MDD), yet remission rates were reported to be below 50%. Methods Since epigenetics of the stress response system seem to play a role in MDD, we analyzed the DNA methylation (DNAm) of genes encoding the glucocorticoid receptor ( NR3C1 ) and proopiomelanocortin ( POMC ) through Sanger Sequencing. For analysis, blood was taken before and after the first and last ECT from MDD patients (n=31), unmedicated depressed controls (UDC; n=19, baseline), and healthy controls (HC; n=20, baseline). Results Baseline DNAm in NR3C1 was significantly lower in UDCs compared to both other groups (UDC: 0.014(±0.002), ECT: 0.031(±0.001), HC: 0.024(±0.002); p<0.001), whereas regarding POMC , ECT patients had the highest DNAm levels (ECT: 0.252(±0.013), UDC: 0.156(±0.015), HC: 0.162(±0.014); p<0.001). NR3C1 m and POMC m decreased after the first ECT ( NR3C1 : p<0.001; POMC : p=0.001), and responders were less methylated compared to non-responders in NR3C1 (p<0.001). Discussion Our findings indicate that both genes might play a role in the chronification of depression and NR3C1 may be relevant for ECT response prediction. |
format | Online Article Text |
id | pubmed-10070046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Georg Thieme Verlag KG |
record_format | MEDLINE/PubMed |
spelling | pubmed-100700462023-04-04 DNA Methylation of POMC and NR3C1-1F and Its Implication in Major Depressive Disorder and Electroconvulsive Therapy Maier, Hannah B. Moschny, Nicole Eberle, Franziska Jahn, Kirsten Folsche, Thorsten Schülke, Rasmus Bleich, Stefan Frieling, Helge Neyazi, Alexandra Pharmacopsychiatry Introduction Precision medicine in psychiatry is still in its infancy. To establish patient-tailored treatment, adequate indicators predicting treatment response are required. Electroconvulsive therapy (ECT) is considered one of the most effective options for pharmacoresistant major depressive disorder (MDD), yet remission rates were reported to be below 50%. Methods Since epigenetics of the stress response system seem to play a role in MDD, we analyzed the DNA methylation (DNAm) of genes encoding the glucocorticoid receptor ( NR3C1 ) and proopiomelanocortin ( POMC ) through Sanger Sequencing. For analysis, blood was taken before and after the first and last ECT from MDD patients (n=31), unmedicated depressed controls (UDC; n=19, baseline), and healthy controls (HC; n=20, baseline). Results Baseline DNAm in NR3C1 was significantly lower in UDCs compared to both other groups (UDC: 0.014(±0.002), ECT: 0.031(±0.001), HC: 0.024(±0.002); p<0.001), whereas regarding POMC , ECT patients had the highest DNAm levels (ECT: 0.252(±0.013), UDC: 0.156(±0.015), HC: 0.162(±0.014); p<0.001). NR3C1 m and POMC m decreased after the first ECT ( NR3C1 : p<0.001; POMC : p=0.001), and responders were less methylated compared to non-responders in NR3C1 (p<0.001). Discussion Our findings indicate that both genes might play a role in the chronification of depression and NR3C1 may be relevant for ECT response prediction. Georg Thieme Verlag KG 2023-03-21 /pmc/articles/PMC10070046/ /pubmed/36944329 http://dx.doi.org/10.1055/a-2034-6536 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/). https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Maier, Hannah B. Moschny, Nicole Eberle, Franziska Jahn, Kirsten Folsche, Thorsten Schülke, Rasmus Bleich, Stefan Frieling, Helge Neyazi, Alexandra DNA Methylation of POMC and NR3C1-1F and Its Implication in Major Depressive Disorder and Electroconvulsive Therapy |
title |
DNA Methylation of
POMC
and
NR3C1-1F
and Its
Implication in Major Depressive Disorder and Electroconvulsive
Therapy
|
title_full |
DNA Methylation of
POMC
and
NR3C1-1F
and Its
Implication in Major Depressive Disorder and Electroconvulsive
Therapy
|
title_fullStr |
DNA Methylation of
POMC
and
NR3C1-1F
and Its
Implication in Major Depressive Disorder and Electroconvulsive
Therapy
|
title_full_unstemmed |
DNA Methylation of
POMC
and
NR3C1-1F
and Its
Implication in Major Depressive Disorder and Electroconvulsive
Therapy
|
title_short |
DNA Methylation of
POMC
and
NR3C1-1F
and Its
Implication in Major Depressive Disorder and Electroconvulsive
Therapy
|
title_sort | dna methylation of
pomc
and
nr3c1-1f
and its
implication in major depressive disorder and electroconvulsive
therapy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070046/ https://www.ncbi.nlm.nih.gov/pubmed/36944329 http://dx.doi.org/10.1055/a-2034-6536 |
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