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Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans

The gut microbiota impacts systemic levels of multiple metabolites including NAD(+) precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD(+) precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypoth...

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Autores principales: Peluso, A. Augusto, Lundgaard, Agnete T., Babaei, Parizad, Mousovich-Neto, Felippe, Rocha, Andréa L., Damgaard, Mads V., Bak, Emilie G., Gnanasekaran, Thiyagarajan, Dollerup, Ole L., Trammell, Samuel A. J., Nielsen, Thomas S., Kern, Timo, Abild, Caroline B., Sulek, Karolina, Ma, Tao, Gerhart-Hines, Zach, Gillum, Matthew P., Arumugam, Manimozhiyan, Ørskov, Cathrine, McCloskey, Douglas, Jessen, Niels, Herrgård, Markus J., Mori, Marcelo A. S., Treebak, Jonas T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070358/
https://www.ncbi.nlm.nih.gov/pubmed/37012386
http://dx.doi.org/10.1038/s41514-023-00106-4
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author Peluso, A. Augusto
Lundgaard, Agnete T.
Babaei, Parizad
Mousovich-Neto, Felippe
Rocha, Andréa L.
Damgaard, Mads V.
Bak, Emilie G.
Gnanasekaran, Thiyagarajan
Dollerup, Ole L.
Trammell, Samuel A. J.
Nielsen, Thomas S.
Kern, Timo
Abild, Caroline B.
Sulek, Karolina
Ma, Tao
Gerhart-Hines, Zach
Gillum, Matthew P.
Arumugam, Manimozhiyan
Ørskov, Cathrine
McCloskey, Douglas
Jessen, Niels
Herrgård, Markus J.
Mori, Marcelo A. S.
Treebak, Jonas T.
author_facet Peluso, A. Augusto
Lundgaard, Agnete T.
Babaei, Parizad
Mousovich-Neto, Felippe
Rocha, Andréa L.
Damgaard, Mads V.
Bak, Emilie G.
Gnanasekaran, Thiyagarajan
Dollerup, Ole L.
Trammell, Samuel A. J.
Nielsen, Thomas S.
Kern, Timo
Abild, Caroline B.
Sulek, Karolina
Ma, Tao
Gerhart-Hines, Zach
Gillum, Matthew P.
Arumugam, Manimozhiyan
Ørskov, Cathrine
McCloskey, Douglas
Jessen, Niels
Herrgård, Markus J.
Mori, Marcelo A. S.
Treebak, Jonas T.
author_sort Peluso, A. Augusto
collection PubMed
description The gut microbiota impacts systemic levels of multiple metabolites including NAD(+) precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD(+) precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypothesized that dietary NR supplementation would modify the gut microbiota across intestinal sections. We determined the effects of 12 weeks of NR supplementation on the microbiota composition of intestinal segments of high-fat diet-fed (HFD) rats. We also explored the effects of 12 weeks of NR supplementation on the gut microbiota in humans and mice. In rats, NR reduced fat mass and tended to decrease body weight. Interestingly, NR increased fat and energy absorption but only in HFD-fed rats. Moreover, 16S rRNA gene sequencing analysis of intestinal and fecal samples revealed an increased abundance of species within Erysipelotrichaceae and Ruminococcaceae families in response to NR. PnuC-positive bacterial strains within these families showed an increased growth rate when supplemented with NR. The abundance of species within the Lachnospiraceae family decreased in response to HFD irrespective of NR. Alpha and beta diversity and bacterial composition of the human fecal microbiota were unaltered by NR, but in mice, the fecal abundance of species within Lachnospiraceae increased while abundances of Parasutterella and Bacteroides dorei species decreased in response to NR. In conclusion, oral NR altered the gut microbiota in rats and mice, but not in humans. In addition, NR attenuated body fat mass gain in rats, and increased fat and energy absorption in the HFD context.
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spelling pubmed-100703582023-04-05 Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans Peluso, A. Augusto Lundgaard, Agnete T. Babaei, Parizad Mousovich-Neto, Felippe Rocha, Andréa L. Damgaard, Mads V. Bak, Emilie G. Gnanasekaran, Thiyagarajan Dollerup, Ole L. Trammell, Samuel A. J. Nielsen, Thomas S. Kern, Timo Abild, Caroline B. Sulek, Karolina Ma, Tao Gerhart-Hines, Zach Gillum, Matthew P. Arumugam, Manimozhiyan Ørskov, Cathrine McCloskey, Douglas Jessen, Niels Herrgård, Markus J. Mori, Marcelo A. S. Treebak, Jonas T. NPJ Aging Article The gut microbiota impacts systemic levels of multiple metabolites including NAD(+) precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD(+) precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypothesized that dietary NR supplementation would modify the gut microbiota across intestinal sections. We determined the effects of 12 weeks of NR supplementation on the microbiota composition of intestinal segments of high-fat diet-fed (HFD) rats. We also explored the effects of 12 weeks of NR supplementation on the gut microbiota in humans and mice. In rats, NR reduced fat mass and tended to decrease body weight. Interestingly, NR increased fat and energy absorption but only in HFD-fed rats. Moreover, 16S rRNA gene sequencing analysis of intestinal and fecal samples revealed an increased abundance of species within Erysipelotrichaceae and Ruminococcaceae families in response to NR. PnuC-positive bacterial strains within these families showed an increased growth rate when supplemented with NR. The abundance of species within the Lachnospiraceae family decreased in response to HFD irrespective of NR. Alpha and beta diversity and bacterial composition of the human fecal microbiota were unaltered by NR, but in mice, the fecal abundance of species within Lachnospiraceae increased while abundances of Parasutterella and Bacteroides dorei species decreased in response to NR. In conclusion, oral NR altered the gut microbiota in rats and mice, but not in humans. In addition, NR attenuated body fat mass gain in rats, and increased fat and energy absorption in the HFD context. Nature Publishing Group UK 2023-04-03 /pmc/articles/PMC10070358/ /pubmed/37012386 http://dx.doi.org/10.1038/s41514-023-00106-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Peluso, A. Augusto
Lundgaard, Agnete T.
Babaei, Parizad
Mousovich-Neto, Felippe
Rocha, Andréa L.
Damgaard, Mads V.
Bak, Emilie G.
Gnanasekaran, Thiyagarajan
Dollerup, Ole L.
Trammell, Samuel A. J.
Nielsen, Thomas S.
Kern, Timo
Abild, Caroline B.
Sulek, Karolina
Ma, Tao
Gerhart-Hines, Zach
Gillum, Matthew P.
Arumugam, Manimozhiyan
Ørskov, Cathrine
McCloskey, Douglas
Jessen, Niels
Herrgård, Markus J.
Mori, Marcelo A. S.
Treebak, Jonas T.
Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
title Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
title_full Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
title_fullStr Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
title_full_unstemmed Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
title_short Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
title_sort oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070358/
https://www.ncbi.nlm.nih.gov/pubmed/37012386
http://dx.doi.org/10.1038/s41514-023-00106-4
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