Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale

The complete chloroplast genome of Secale cereale ssp. segetale (Zhuk.) Roshev. (Poaceae: Triticeae) was sequenced and analyzed to better use its genetic resources to enrich rye and wheat breeding. The study was carried out using the following methods: DNA extraction, sequencing, assembly and annota...

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Autores principales: Skuza, Lidia, Androsiuk, Piotr, Gastineau, Romain, Paukszto, Łukasz, Jastrzębski, Jan Paweł, Cembrowska-Lech, Danuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070434/
https://www.ncbi.nlm.nih.gov/pubmed/37012409
http://dx.doi.org/10.1038/s41598-023-32587-4
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author Skuza, Lidia
Androsiuk, Piotr
Gastineau, Romain
Paukszto, Łukasz
Jastrzębski, Jan Paweł
Cembrowska-Lech, Danuta
author_facet Skuza, Lidia
Androsiuk, Piotr
Gastineau, Romain
Paukszto, Łukasz
Jastrzębski, Jan Paweł
Cembrowska-Lech, Danuta
author_sort Skuza, Lidia
collection PubMed
description The complete chloroplast genome of Secale cereale ssp. segetale (Zhuk.) Roshev. (Poaceae: Triticeae) was sequenced and analyzed to better use its genetic resources to enrich rye and wheat breeding. The study was carried out using the following methods: DNA extraction, sequencing, assembly and annotation, comparison with other complete chloroplast genomes of the five Secale species, and multigene phylogeny. As a result of the study, it was determined that the chloroplast genome is 137,042 base pair (bp) long and contains 137 genes, including 113 unique genes and 24 genes which are duplicated in the IRs. Moreover, a total of 29 SSRs were detected in the Secale cereale ssp. segetale chloroplast genome. The phylogenetic analysis showed that Secale cereale ssp. segetale appeared to share the highest degree of similarity with S. cereale and S. strictum. Intraspecific diversity has been observed between the published chloroplast genome sequences of S. cereale ssp. segetale. The genome can be accessed on GenBank with the accession number (OL688773).
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spelling pubmed-100704342023-04-05 Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale Skuza, Lidia Androsiuk, Piotr Gastineau, Romain Paukszto, Łukasz Jastrzębski, Jan Paweł Cembrowska-Lech, Danuta Sci Rep Article The complete chloroplast genome of Secale cereale ssp. segetale (Zhuk.) Roshev. (Poaceae: Triticeae) was sequenced and analyzed to better use its genetic resources to enrich rye and wheat breeding. The study was carried out using the following methods: DNA extraction, sequencing, assembly and annotation, comparison with other complete chloroplast genomes of the five Secale species, and multigene phylogeny. As a result of the study, it was determined that the chloroplast genome is 137,042 base pair (bp) long and contains 137 genes, including 113 unique genes and 24 genes which are duplicated in the IRs. Moreover, a total of 29 SSRs were detected in the Secale cereale ssp. segetale chloroplast genome. The phylogenetic analysis showed that Secale cereale ssp. segetale appeared to share the highest degree of similarity with S. cereale and S. strictum. Intraspecific diversity has been observed between the published chloroplast genome sequences of S. cereale ssp. segetale. The genome can be accessed on GenBank with the accession number (OL688773). Nature Publishing Group UK 2023-04-03 /pmc/articles/PMC10070434/ /pubmed/37012409 http://dx.doi.org/10.1038/s41598-023-32587-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Skuza, Lidia
Androsiuk, Piotr
Gastineau, Romain
Paukszto, Łukasz
Jastrzębski, Jan Paweł
Cembrowska-Lech, Danuta
Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale
title Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale
title_full Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale
title_fullStr Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale
title_full_unstemmed Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale
title_short Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale
title_sort molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye secale cereale ssp. segetale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070434/
https://www.ncbi.nlm.nih.gov/pubmed/37012409
http://dx.doi.org/10.1038/s41598-023-32587-4
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