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Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf va...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070790/ https://www.ncbi.nlm.nih.gov/pubmed/37026014 http://dx.doi.org/10.3389/fimmu.2023.1077938 |
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author | Wang, Yan Sun, Shihui Zhao, Kui Du, Le Wang, Xinyue He, Wenqi Gao, Feng Song, Deguang Guan, Jiyu |
author_facet | Wang, Yan Sun, Shihui Zhao, Kui Du, Le Wang, Xinyue He, Wenqi Gao, Feng Song, Deguang Guan, Jiyu |
author_sort | Wang, Yan |
collection | PubMed |
description | Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy. |
format | Online Article Text |
id | pubmed-10070790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100707902023-04-05 Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep Wang, Yan Sun, Shihui Zhao, Kui Du, Le Wang, Xinyue He, Wenqi Gao, Feng Song, Deguang Guan, Jiyu Front Immunol Immunology Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy. Frontiers Media S.A. 2023-03-21 /pmc/articles/PMC10070790/ /pubmed/37026014 http://dx.doi.org/10.3389/fimmu.2023.1077938 Text en Copyright © 2023 Wang, Sun, Zhao, Du, Wang, He, Gao, Song and Guan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Wang, Yan Sun, Shihui Zhao, Kui Du, Le Wang, Xinyue He, Wenqi Gao, Feng Song, Deguang Guan, Jiyu Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
title | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
title_full | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
title_fullStr | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
title_full_unstemmed | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
title_short | Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
title_sort | orf virus dna prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070790/ https://www.ncbi.nlm.nih.gov/pubmed/37026014 http://dx.doi.org/10.3389/fimmu.2023.1077938 |
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