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Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep

Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf va...

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Autores principales: Wang, Yan, Sun, Shihui, Zhao, Kui, Du, Le, Wang, Xinyue, He, Wenqi, Gao, Feng, Song, Deguang, Guan, Jiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070790/
https://www.ncbi.nlm.nih.gov/pubmed/37026014
http://dx.doi.org/10.3389/fimmu.2023.1077938
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author Wang, Yan
Sun, Shihui
Zhao, Kui
Du, Le
Wang, Xinyue
He, Wenqi
Gao, Feng
Song, Deguang
Guan, Jiyu
author_facet Wang, Yan
Sun, Shihui
Zhao, Kui
Du, Le
Wang, Xinyue
He, Wenqi
Gao, Feng
Song, Deguang
Guan, Jiyu
author_sort Wang, Yan
collection PubMed
description Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy.
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spelling pubmed-100707902023-04-05 Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep Wang, Yan Sun, Shihui Zhao, Kui Du, Le Wang, Xinyue He, Wenqi Gao, Feng Song, Deguang Guan, Jiyu Front Immunol Immunology Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy. Frontiers Media S.A. 2023-03-21 /pmc/articles/PMC10070790/ /pubmed/37026014 http://dx.doi.org/10.3389/fimmu.2023.1077938 Text en Copyright © 2023 Wang, Sun, Zhao, Du, Wang, He, Gao, Song and Guan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wang, Yan
Sun, Shihui
Zhao, Kui
Du, Le
Wang, Xinyue
He, Wenqi
Gao, Feng
Song, Deguang
Guan, Jiyu
Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
title Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
title_full Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
title_fullStr Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
title_full_unstemmed Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
title_short Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
title_sort orf virus dna prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070790/
https://www.ncbi.nlm.nih.gov/pubmed/37026014
http://dx.doi.org/10.3389/fimmu.2023.1077938
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