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Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa

Cannabis sativa is a multi-use and chemically complex plant which is utilized for food, fiber, and medicine. Plants produce a class of psychoactive and medicinally important specialized metabolites referred to as phytocannabinoids (PCs). The phytohormone methyl jasmonate (MeJA) is a naturally occurr...

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Autores principales: Welling, Matthew T., Deseo, Myrna A., O’Brien, Martin, Clifton, Jacob, Bacic, Antony, Doblin, Monika S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070988/
https://www.ncbi.nlm.nih.gov/pubmed/37025140
http://dx.doi.org/10.3389/fpls.2023.1110144
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author Welling, Matthew T.
Deseo, Myrna A.
O’Brien, Martin
Clifton, Jacob
Bacic, Antony
Doblin, Monika S.
author_facet Welling, Matthew T.
Deseo, Myrna A.
O’Brien, Martin
Clifton, Jacob
Bacic, Antony
Doblin, Monika S.
author_sort Welling, Matthew T.
collection PubMed
description Cannabis sativa is a multi-use and chemically complex plant which is utilized for food, fiber, and medicine. Plants produce a class of psychoactive and medicinally important specialized metabolites referred to as phytocannabinoids (PCs). The phytohormone methyl jasmonate (MeJA) is a naturally occurring methyl ester of jasmonic acid and a product of oxylipin biosynthesis which initiates and regulates the biosynthesis of a broad range of specialized metabolites across a number of diverse plant lineages. While the effects of exogenous MeJA application on PC production has been reported, treatments have been constrained to a narrow molar range and to the targeted analysis of a small number of compounds. Using high-resolution mass spectrometry with data-dependent acquisition, we examined the global metabolomic effects of MeJA in C. sativa to explore oxylipin-mediated regulation of PC biosynthesis and accumulation. A dose–response relationship was observed, with an almost two-fold increase in PC content found in inflorescences of female clones treated with 15 mM MeJA compared to the control group. Comparison of the inflorescence metabolome across MeJA treatments coupled with targeted transcript analysis was used to elucidate key regulatory components contributing to PC production and metabolism more broadly. Revealing these biological signatures improves our understanding of the role of the oxylipin pathway in C. sativa and provides putative molecular targets for the metabolic engineering and optimization of chemical phenotype for medicinal and industrial end-uses.
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spelling pubmed-100709882023-04-05 Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa Welling, Matthew T. Deseo, Myrna A. O’Brien, Martin Clifton, Jacob Bacic, Antony Doblin, Monika S. Front Plant Sci Plant Science Cannabis sativa is a multi-use and chemically complex plant which is utilized for food, fiber, and medicine. Plants produce a class of psychoactive and medicinally important specialized metabolites referred to as phytocannabinoids (PCs). The phytohormone methyl jasmonate (MeJA) is a naturally occurring methyl ester of jasmonic acid and a product of oxylipin biosynthesis which initiates and regulates the biosynthesis of a broad range of specialized metabolites across a number of diverse plant lineages. While the effects of exogenous MeJA application on PC production has been reported, treatments have been constrained to a narrow molar range and to the targeted analysis of a small number of compounds. Using high-resolution mass spectrometry with data-dependent acquisition, we examined the global metabolomic effects of MeJA in C. sativa to explore oxylipin-mediated regulation of PC biosynthesis and accumulation. A dose–response relationship was observed, with an almost two-fold increase in PC content found in inflorescences of female clones treated with 15 mM MeJA compared to the control group. Comparison of the inflorescence metabolome across MeJA treatments coupled with targeted transcript analysis was used to elucidate key regulatory components contributing to PC production and metabolism more broadly. Revealing these biological signatures improves our understanding of the role of the oxylipin pathway in C. sativa and provides putative molecular targets for the metabolic engineering and optimization of chemical phenotype for medicinal and industrial end-uses. Frontiers Media S.A. 2023-03-21 /pmc/articles/PMC10070988/ /pubmed/37025140 http://dx.doi.org/10.3389/fpls.2023.1110144 Text en Copyright © 2023 Welling, Deseo, O’Brien, Clifton, Bacic and Doblin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Welling, Matthew T.
Deseo, Myrna A.
O’Brien, Martin
Clifton, Jacob
Bacic, Antony
Doblin, Monika S.
Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa
title Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa
title_full Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa
title_fullStr Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa
title_full_unstemmed Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa
title_short Metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in Cannabis sativa
title_sort metabolomic analysis of methyl jasmonate treatment on phytocannabinoid production in cannabis sativa
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070988/
https://www.ncbi.nlm.nih.gov/pubmed/37025140
http://dx.doi.org/10.3389/fpls.2023.1110144
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