Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells

CRISPR/Cas9‐mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock‐in (KI) efficiency is critical for fully implementing TI‐medi...

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Detalles Bibliográficos
Autores principales: Kim, Seul Mi, Lee, Jaejin, Lee, Jae Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10071570/
https://www.ncbi.nlm.nih.gov/pubmed/37025191
http://dx.doi.org/10.1002/elsc.202200047

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10071570/
https://www.ncbi.nlm.nih.gov/pubmed/37025191
http://dx.doi.org/10.1002/elsc.202200047

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