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Transmembrane transport of fluoride studied by time-resolved emission spectroscopy

Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(iii) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of t...

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Detalles Bibliográficos
Autores principales: Cataldo, Alessio, Chvojka, Matúš, Park, Gyeongjin, Šindelář, Vladimír, Gabbaï, François P., Butler, Stephen J., Valkenier, Hennie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072081/
https://www.ncbi.nlm.nih.gov/pubmed/36938842
http://dx.doi.org/10.1039/d3cc00897e
Descripción
Sumario:Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(iii) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action.