Transmembrane transport of fluoride studied by time-resolved emission spectroscopy

Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(iii) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of t...

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Detalles Bibliográficos
Autores principales: Cataldo, Alessio, Chvojka, Matúš, Park, Gyeongjin, Šindelář, Vladimír, Gabbaï, François P., Butler, Stephen J., Valkenier, Hennie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072081/
https://www.ncbi.nlm.nih.gov/pubmed/36938842
http://dx.doi.org/10.1039/d3cc00897e
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author Cataldo, Alessio
Chvojka, Matúš
Park, Gyeongjin
Šindelář, Vladimír
Gabbaï, François P.
Butler, Stephen J.
Valkenier, Hennie
author_facet Cataldo, Alessio
Chvojka, Matúš
Park, Gyeongjin
Šindelář, Vladimír
Gabbaï, François P.
Butler, Stephen J.
Valkenier, Hennie
author_sort Cataldo, Alessio
collection PubMed
description Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(iii) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action.
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spelling pubmed-100720812023-04-05 Transmembrane transport of fluoride studied by time-resolved emission spectroscopy Cataldo, Alessio Chvojka, Matúš Park, Gyeongjin Šindelář, Vladimír Gabbaï, François P. Butler, Stephen J. Valkenier, Hennie Chem Commun (Camb) Chemistry Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(iii) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action. The Royal Society of Chemistry 2023-03-14 /pmc/articles/PMC10072081/ /pubmed/36938842 http://dx.doi.org/10.1039/d3cc00897e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Cataldo, Alessio
Chvojka, Matúš
Park, Gyeongjin
Šindelář, Vladimír
Gabbaï, François P.
Butler, Stephen J.
Valkenier, Hennie
Transmembrane transport of fluoride studied by time-resolved emission spectroscopy
title Transmembrane transport of fluoride studied by time-resolved emission spectroscopy
title_full Transmembrane transport of fluoride studied by time-resolved emission spectroscopy
title_fullStr Transmembrane transport of fluoride studied by time-resolved emission spectroscopy
title_full_unstemmed Transmembrane transport of fluoride studied by time-resolved emission spectroscopy
title_short Transmembrane transport of fluoride studied by time-resolved emission spectroscopy
title_sort transmembrane transport of fluoride studied by time-resolved emission spectroscopy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072081/
https://www.ncbi.nlm.nih.gov/pubmed/36938842
http://dx.doi.org/10.1039/d3cc00897e
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