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Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1

Crimean-Congo hemorrhagic fever virus (CCHFV) can cause severe hemorrhagic fever in humans and is mainly transmitted by ticks. There is no effective vaccine for Crimean-Congo hemorrhagic fever (CCHF) at present. We developed three DNA vaccines encoding CCHFV nucleocapsid protein (NP), glycoprotein N...

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Autores principales: Hu, Yong-Liang, Zhang, Lian-Qing, Liu, Xiao-Qian, Ye, Wei, Zhao, Yue-Xi, Zhang, Liang, Qiang, Zun-Xian, Zhang, Lin-Xuan, Lei, Ying-Feng, Jiang, Dong-Bo, Cheng, Lin-Feng, Zhang, Fang-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072157/
https://www.ncbi.nlm.nih.gov/pubmed/37026060
http://dx.doi.org/10.3389/fcimb.2023.1121163
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author Hu, Yong-Liang
Zhang, Lian-Qing
Liu, Xiao-Qian
Ye, Wei
Zhao, Yue-Xi
Zhang, Liang
Qiang, Zun-Xian
Zhang, Lin-Xuan
Lei, Ying-Feng
Jiang, Dong-Bo
Cheng, Lin-Feng
Zhang, Fang-Lin
author_facet Hu, Yong-Liang
Zhang, Lian-Qing
Liu, Xiao-Qian
Ye, Wei
Zhao, Yue-Xi
Zhang, Liang
Qiang, Zun-Xian
Zhang, Lin-Xuan
Lei, Ying-Feng
Jiang, Dong-Bo
Cheng, Lin-Feng
Zhang, Fang-Lin
author_sort Hu, Yong-Liang
collection PubMed
description Crimean-Congo hemorrhagic fever virus (CCHFV) can cause severe hemorrhagic fever in humans and is mainly transmitted by ticks. There is no effective vaccine for Crimean-Congo hemorrhagic fever (CCHF) at present. We developed three DNA vaccines encoding CCHFV nucleocapsid protein (NP), glycoprotein N-terminal (Gn) and C-terminal (Gc) fused with lysosome-associated membrane protein 1 (LAMP1) and assessed their immunogenicity and protective efficacy in a human MHC (HLA-A11/DR1) transgenic mouse model. The mice that were vaccinated three times with pVAX-LAMP1-CCHFV-NP induced balanced Th1 and Th2 responses and could most effectively protect mice from CCHFV transcription and entry-competent virus-like particles (tecVLPs) infection. The mice vaccinated with pVAX-LAMP1-CCHFV-Gc mainly elicited specific anti-Gc and neutralizing antibodies and provided a certain protection from CCHFV tecVLPs infection, but the protective efficacy was less than that of pVAX-LAMP1-CCHFV-NP. The mice vaccinated with pVAX-LAMP1-CCHFV-Gn only elicited specific anti-Gn antibodies and could not provide sufficient protection from CCHFV tecVLPs infection. These results suggest that pVAX-LAMP1-CCHFV-NP would be a potential and powerful candidate vaccine for CCHFV.
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spelling pubmed-100721572023-04-05 Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1 Hu, Yong-Liang Zhang, Lian-Qing Liu, Xiao-Qian Ye, Wei Zhao, Yue-Xi Zhang, Liang Qiang, Zun-Xian Zhang, Lin-Xuan Lei, Ying-Feng Jiang, Dong-Bo Cheng, Lin-Feng Zhang, Fang-Lin Front Cell Infect Microbiol Cellular and Infection Microbiology Crimean-Congo hemorrhagic fever virus (CCHFV) can cause severe hemorrhagic fever in humans and is mainly transmitted by ticks. There is no effective vaccine for Crimean-Congo hemorrhagic fever (CCHF) at present. We developed three DNA vaccines encoding CCHFV nucleocapsid protein (NP), glycoprotein N-terminal (Gn) and C-terminal (Gc) fused with lysosome-associated membrane protein 1 (LAMP1) and assessed their immunogenicity and protective efficacy in a human MHC (HLA-A11/DR1) transgenic mouse model. The mice that were vaccinated three times with pVAX-LAMP1-CCHFV-NP induced balanced Th1 and Th2 responses and could most effectively protect mice from CCHFV transcription and entry-competent virus-like particles (tecVLPs) infection. The mice vaccinated with pVAX-LAMP1-CCHFV-Gc mainly elicited specific anti-Gc and neutralizing antibodies and provided a certain protection from CCHFV tecVLPs infection, but the protective efficacy was less than that of pVAX-LAMP1-CCHFV-NP. The mice vaccinated with pVAX-LAMP1-CCHFV-Gn only elicited specific anti-Gn antibodies and could not provide sufficient protection from CCHFV tecVLPs infection. These results suggest that pVAX-LAMP1-CCHFV-NP would be a potential and powerful candidate vaccine for CCHFV. Frontiers Media S.A. 2023-03-21 /pmc/articles/PMC10072157/ /pubmed/37026060 http://dx.doi.org/10.3389/fcimb.2023.1121163 Text en Copyright © 2023 Hu, Zhang, Liu, Ye, Zhao, Zhang, Qiang, Zhang, Lei, Jiang, Cheng and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Hu, Yong-Liang
Zhang, Lian-Qing
Liu, Xiao-Qian
Ye, Wei
Zhao, Yue-Xi
Zhang, Liang
Qiang, Zun-Xian
Zhang, Lin-Xuan
Lei, Ying-Feng
Jiang, Dong-Bo
Cheng, Lin-Feng
Zhang, Fang-Lin
Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
title Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
title_full Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
title_fullStr Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
title_full_unstemmed Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
title_short Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
title_sort construction and evaluation of dna vaccine encoding crimean congo hemorrhagic fever virus nucleocapsid protein, glycoprotein n-terminal and c-terminal fused with lamp1
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072157/
https://www.ncbi.nlm.nih.gov/pubmed/37026060
http://dx.doi.org/10.3389/fcimb.2023.1121163
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