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The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation

Oxidative phenomena are considered to lie at the root of the accelerated senescence observed in red blood cells (RBCs) stored under standard blood bank conditions. It was recently shown that the addition of uric (UA) and/or ascorbic acid (AA) to the preservative medium beneficially impacts the stora...

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Autores principales: Anastasiadi, Alkmini T., Stamoulis, Konstantinos, Papageorgiou, Effie G., Lelli, Veronica, Rinalducci, Sara, Papassideri, Issidora S., Kriebardis, Anastasios G., Antonelou, Marianna H., Tzounakas, Vassilis L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072267/
https://www.ncbi.nlm.nih.gov/pubmed/37025499
http://dx.doi.org/10.3389/fragi.2023.1161565
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author Anastasiadi, Alkmini T.
Stamoulis, Konstantinos
Papageorgiou, Effie G.
Lelli, Veronica
Rinalducci, Sara
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou, Marianna H.
Tzounakas, Vassilis L.
author_facet Anastasiadi, Alkmini T.
Stamoulis, Konstantinos
Papageorgiou, Effie G.
Lelli, Veronica
Rinalducci, Sara
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou, Marianna H.
Tzounakas, Vassilis L.
author_sort Anastasiadi, Alkmini T.
collection PubMed
description Oxidative phenomena are considered to lie at the root of the accelerated senescence observed in red blood cells (RBCs) stored under standard blood bank conditions. It was recently shown that the addition of uric (UA) and/or ascorbic acid (AA) to the preservative medium beneficially impacts the storability features of RBCs related to the handling of pro-oxidant triggers. This study constitutes the next step, aiming to examine the links between hemolysis, redox, and metabolic parameters in control and supplemented RBC units of different storage times. For this purpose, a paired correlation analysis of physiological and metabolism parameters was performed between early, middle, and late storage in each subgroup. Strong and repeated correlations were observed throughout storage in most hemolysis parameters, as well as in reactive oxygen species (ROS) and lipid peroxidation, suggesting that these features constitute donor-signatures, unaffected by the diverse storage solutions. Moreover, during storage, a general “dialogue” was observed between parameters of the same category (e.g., cell fragilities and hemolysis or lipid peroxidation and ROS), highlighting their interdependence. In all groups, extracellular antioxidant capacity, proteasomal activity, and glutathione precursors of preceding time points anticorrelated with oxidative stress lesions of upcoming ones. In the case of supplemented units, factors responsible for glutathione synthesis varied proportionally to the levels of glutathione itself. The current findings support that UA and AA addition reroutes the metabolism to induce glutathione production, and additionally provide mechanistic insight and footing to examine novel storage optimization strategies.
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spelling pubmed-100722672023-04-05 The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation Anastasiadi, Alkmini T. Stamoulis, Konstantinos Papageorgiou, Effie G. Lelli, Veronica Rinalducci, Sara Papassideri, Issidora S. Kriebardis, Anastasios G. Antonelou, Marianna H. Tzounakas, Vassilis L. Front Aging Aging Oxidative phenomena are considered to lie at the root of the accelerated senescence observed in red blood cells (RBCs) stored under standard blood bank conditions. It was recently shown that the addition of uric (UA) and/or ascorbic acid (AA) to the preservative medium beneficially impacts the storability features of RBCs related to the handling of pro-oxidant triggers. This study constitutes the next step, aiming to examine the links between hemolysis, redox, and metabolic parameters in control and supplemented RBC units of different storage times. For this purpose, a paired correlation analysis of physiological and metabolism parameters was performed between early, middle, and late storage in each subgroup. Strong and repeated correlations were observed throughout storage in most hemolysis parameters, as well as in reactive oxygen species (ROS) and lipid peroxidation, suggesting that these features constitute donor-signatures, unaffected by the diverse storage solutions. Moreover, during storage, a general “dialogue” was observed between parameters of the same category (e.g., cell fragilities and hemolysis or lipid peroxidation and ROS), highlighting their interdependence. In all groups, extracellular antioxidant capacity, proteasomal activity, and glutathione precursors of preceding time points anticorrelated with oxidative stress lesions of upcoming ones. In the case of supplemented units, factors responsible for glutathione synthesis varied proportionally to the levels of glutathione itself. The current findings support that UA and AA addition reroutes the metabolism to induce glutathione production, and additionally provide mechanistic insight and footing to examine novel storage optimization strategies. Frontiers Media S.A. 2023-03-21 /pmc/articles/PMC10072267/ /pubmed/37025499 http://dx.doi.org/10.3389/fragi.2023.1161565 Text en Copyright © 2023 Anastasiadi, Stamoulis, Papageorgiou, Lelli, Rinalducci, Papassideri, Kriebardis, Antonelou and Tzounakas. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging
Anastasiadi, Alkmini T.
Stamoulis, Konstantinos
Papageorgiou, Effie G.
Lelli, Veronica
Rinalducci, Sara
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou, Marianna H.
Tzounakas, Vassilis L.
The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
title The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
title_full The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
title_fullStr The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
title_full_unstemmed The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
title_short The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
title_sort time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation
topic Aging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072267/
https://www.ncbi.nlm.nih.gov/pubmed/37025499
http://dx.doi.org/10.3389/fragi.2023.1161565
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