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Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes

Oomycetes are filamentous microorganisms easily mistaken as fungi but vastly differ in physiology, biochemistry, and genetics. This commonly-held misconception lead to a reduced effectiveness by using conventional fungicides to control oomycetes, thus it demands the identification of novel functiona...

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Autores principales: Qiu, Min, Tian, Mengjun, Yong, Saijiang, Sun, Yaru, Cao, Jingting, Li, Yaning, Zhang, Xin, Zhai, Chunhua, Ye, Wenwu, Wang, Ming, Wang, Yuanchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072465/
https://www.ncbi.nlm.nih.gov/pubmed/36952577
http://dx.doi.org/10.1371/journal.ppat.1011256
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author Qiu, Min
Tian, Mengjun
Yong, Saijiang
Sun, Yaru
Cao, Jingting
Li, Yaning
Zhang, Xin
Zhai, Chunhua
Ye, Wenwu
Wang, Ming
Wang, Yuanchao
author_facet Qiu, Min
Tian, Mengjun
Yong, Saijiang
Sun, Yaru
Cao, Jingting
Li, Yaning
Zhang, Xin
Zhai, Chunhua
Ye, Wenwu
Wang, Ming
Wang, Yuanchao
author_sort Qiu, Min
collection PubMed
description Oomycetes are filamentous microorganisms easily mistaken as fungi but vastly differ in physiology, biochemistry, and genetics. This commonly-held misconception lead to a reduced effectiveness by using conventional fungicides to control oomycetes, thus it demands the identification of novel functional genes as target for precisely design oomycetes-specific microbicide. The present study initially analyzed the available transcriptome data of the model oomycete pathogen, Phytophthora sojae, and constructed an expression matrix of 10,953 genes across the stages of asexual development and host infection. Hierarchical clustering, specificity, and diversity analyses revealed a more pronounced transcriptional plasticity during the stages of asexual development than that in host infection, which drew our attention by particularly focusing on transcripts in asexual development stage to eventually clustered them into 6 phase-specific expression modules. Three of which respectively possessing a serine/threonine phosphatase (PP2C) expressed during the mycelial and sporangium stages, a histidine kinase (HK) expressed during the zoospore and cyst stages, and a bZIP transcription factor (bZIP32) exclusive to the cyst germination stage were selected for down-stream functional validation. In this way, we demonstrated that PP2C, HK, and bZIP32 play significant roles in P. sojae asexual development and virulence. Thus, these findings provide a foundation for further gene functional annotation in oomycetes and crop disease management.
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spelling pubmed-100724652023-04-05 Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes Qiu, Min Tian, Mengjun Yong, Saijiang Sun, Yaru Cao, Jingting Li, Yaning Zhang, Xin Zhai, Chunhua Ye, Wenwu Wang, Ming Wang, Yuanchao PLoS Pathog Research Article Oomycetes are filamentous microorganisms easily mistaken as fungi but vastly differ in physiology, biochemistry, and genetics. This commonly-held misconception lead to a reduced effectiveness by using conventional fungicides to control oomycetes, thus it demands the identification of novel functional genes as target for precisely design oomycetes-specific microbicide. The present study initially analyzed the available transcriptome data of the model oomycete pathogen, Phytophthora sojae, and constructed an expression matrix of 10,953 genes across the stages of asexual development and host infection. Hierarchical clustering, specificity, and diversity analyses revealed a more pronounced transcriptional plasticity during the stages of asexual development than that in host infection, which drew our attention by particularly focusing on transcripts in asexual development stage to eventually clustered them into 6 phase-specific expression modules. Three of which respectively possessing a serine/threonine phosphatase (PP2C) expressed during the mycelial and sporangium stages, a histidine kinase (HK) expressed during the zoospore and cyst stages, and a bZIP transcription factor (bZIP32) exclusive to the cyst germination stage were selected for down-stream functional validation. In this way, we demonstrated that PP2C, HK, and bZIP32 play significant roles in P. sojae asexual development and virulence. Thus, these findings provide a foundation for further gene functional annotation in oomycetes and crop disease management. Public Library of Science 2023-03-23 /pmc/articles/PMC10072465/ /pubmed/36952577 http://dx.doi.org/10.1371/journal.ppat.1011256 Text en © 2023 Qiu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Qiu, Min
Tian, Mengjun
Yong, Saijiang
Sun, Yaru
Cao, Jingting
Li, Yaning
Zhang, Xin
Zhai, Chunhua
Ye, Wenwu
Wang, Ming
Wang, Yuanchao
Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes
title Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes
title_full Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes
title_fullStr Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes
title_full_unstemmed Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes
title_short Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes
title_sort phase-specific transcriptional patterns of the oomycete pathogen phytophthora sojae unravel genes essential for asexual development and pathogenic processes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072465/
https://www.ncbi.nlm.nih.gov/pubmed/36952577
http://dx.doi.org/10.1371/journal.ppat.1011256
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