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Exploiting volumetric wave correlation for enhanced depth imaging in scattering medium

Imaging an object embedded within a scattering medium requires the correction of complex sample-induced wave distortions. Existing approaches have been designed to resolve them by optimizing signal waves recorded in each 2D image. Here, we present a volumetric image reconstruction framework that mer...

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Detalles Bibliográficos
Autores principales: Lee, Ye-Ryoung, Kim, Dong-Young, Jo, Yonghyeon, Kim, Moonseok, Choi, Wonshik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073116/
https://www.ncbi.nlm.nih.gov/pubmed/37015941
http://dx.doi.org/10.1038/s41467-023-37467-z
Descripción
Sumario:Imaging an object embedded within a scattering medium requires the correction of complex sample-induced wave distortions. Existing approaches have been designed to resolve them by optimizing signal waves recorded in each 2D image. Here, we present a volumetric image reconstruction framework that merges two fundamental degrees of freedom, the wavelength and propagation angles of light waves, based on the object momentum conservation principle. On this basis, we propose methods for exploiting the correlation of signal waves from volumetric images to better cope with multiple scattering. By constructing experimental systems scanning both wavelength and illumination angle of the light source, we demonstrated a 32-fold increase in the use of signal waves compared with that of existing 2D-based approaches and achieved ultrahigh volumetric resolution (lateral resolution: 0.41 [Formula: see text] , axial resolution: 0.60 [Formula: see text] ) even within complex scattering medium owing to the optimal coherent use of the broad spectral bandwidth (225 nm).