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Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia

Infectious diseases caused by filarial nematodes are major health problems for humans and animals globally. Current treatment using anti-helminthic drugs requires a long treatment period and is only effective against the microfilarial stage. Most species of filarial nematodes harbor a specific strai...

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Autores principales: Wangwiwatsin, Arporn, Kulwong, Siriyakorn, Phetcharaburanin, Jutarop, Namwat, Nisana, Klanrit, Poramate, Loilome, Watcharin, Maleewong, Wanchai, Reid, Adam J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073474/
https://www.ncbi.nlm.nih.gov/pubmed/37032902
http://dx.doi.org/10.3389/fmicb.2023.1052352
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author Wangwiwatsin, Arporn
Kulwong, Siriyakorn
Phetcharaburanin, Jutarop
Namwat, Nisana
Klanrit, Poramate
Loilome, Watcharin
Maleewong, Wanchai
Reid, Adam J.
author_facet Wangwiwatsin, Arporn
Kulwong, Siriyakorn
Phetcharaburanin, Jutarop
Namwat, Nisana
Klanrit, Poramate
Loilome, Watcharin
Maleewong, Wanchai
Reid, Adam J.
author_sort Wangwiwatsin, Arporn
collection PubMed
description Infectious diseases caused by filarial nematodes are major health problems for humans and animals globally. Current treatment using anti-helminthic drugs requires a long treatment period and is only effective against the microfilarial stage. Most species of filarial nematodes harbor a specific strain of Wolbachia bacteria, which are essential for the survival, development, and reproduction of the nematodes. This parasite-bacteria obligate symbiosis offers a new angle for the cure of filariasis. In this study, we utilized publicly available genome data and putative protein sequences from seven filarial nematode species and their symbiotic Wolbachia to screen for protein–protein interactions that could be a novel target against multiple filarial nematode species. Genome-wide in silico screening was performed to predict molecular interactions based on co-evolutionary signals. We identified over 8,000 pairs of gene families that show evidence of co-evolution based on high correlation score and low false discovery rate (FDR) between gene families and obtained a candidate list that may be keys in filarial nematode–Wolbachia interactions. Functional analysis was conducted on these top-scoring pairs, revealing biological processes related to various signaling processes, adult lifespan, developmental control, lipid and nucleotide metabolism, and RNA modification. Furthermore, network analysis of the top-scoring genes with multiple co-evolving pairs suggests candidate genes in both Wolbachia and the nematode that may play crucial roles at the center of multi-gene networks. A number of the top-scoring genes matched well to known drug targets, suggesting a promising drug-repurposing strategy that could be applicable against multiple filarial nematode species.
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spelling pubmed-100734742023-04-06 Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia Wangwiwatsin, Arporn Kulwong, Siriyakorn Phetcharaburanin, Jutarop Namwat, Nisana Klanrit, Poramate Loilome, Watcharin Maleewong, Wanchai Reid, Adam J. Front Microbiol Microbiology Infectious diseases caused by filarial nematodes are major health problems for humans and animals globally. Current treatment using anti-helminthic drugs requires a long treatment period and is only effective against the microfilarial stage. Most species of filarial nematodes harbor a specific strain of Wolbachia bacteria, which are essential for the survival, development, and reproduction of the nematodes. This parasite-bacteria obligate symbiosis offers a new angle for the cure of filariasis. In this study, we utilized publicly available genome data and putative protein sequences from seven filarial nematode species and their symbiotic Wolbachia to screen for protein–protein interactions that could be a novel target against multiple filarial nematode species. Genome-wide in silico screening was performed to predict molecular interactions based on co-evolutionary signals. We identified over 8,000 pairs of gene families that show evidence of co-evolution based on high correlation score and low false discovery rate (FDR) between gene families and obtained a candidate list that may be keys in filarial nematode–Wolbachia interactions. Functional analysis was conducted on these top-scoring pairs, revealing biological processes related to various signaling processes, adult lifespan, developmental control, lipid and nucleotide metabolism, and RNA modification. Furthermore, network analysis of the top-scoring genes with multiple co-evolving pairs suggests candidate genes in both Wolbachia and the nematode that may play crucial roles at the center of multi-gene networks. A number of the top-scoring genes matched well to known drug targets, suggesting a promising drug-repurposing strategy that could be applicable against multiple filarial nematode species. Frontiers Media S.A. 2023-03-22 /pmc/articles/PMC10073474/ /pubmed/37032902 http://dx.doi.org/10.3389/fmicb.2023.1052352 Text en Copyright © 2023 Wangwiwatsin, Kulwong, Phetcharaburanin, Namwat, Klanrit, Loilome, Maleewong and Reid. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wangwiwatsin, Arporn
Kulwong, Siriyakorn
Phetcharaburanin, Jutarop
Namwat, Nisana
Klanrit, Poramate
Loilome, Watcharin
Maleewong, Wanchai
Reid, Adam J.
Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia
title Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia
title_full Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia
title_fullStr Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia
title_full_unstemmed Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia
title_short Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia
title_sort toward novel treatment against filariasis: insight into genome-wide co-evolutionary analysis of filarial nematodes and wolbachia
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073474/
https://www.ncbi.nlm.nih.gov/pubmed/37032902
http://dx.doi.org/10.3389/fmicb.2023.1052352
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