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Association between the vaginal and uterine microbiota and the risk of early embryonic arrest

The aim of this study was to explore the microecological distribution and differences in the uterus and vaginal microbiome in women with early embryonic arrest and those with normal pregnancy by high-throughput sequencing. We systematically sampled the vaginal and uterine microbiomes of 56 pregnant...

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Autores principales: Wang, Li, Chen, Junyu, He, Lin, Liu, Hanbo, Liu, Yan, Luan, Zonghui, Li, Hong, Liu, Weixin, Luo, Mengjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073571/
https://www.ncbi.nlm.nih.gov/pubmed/37032888
http://dx.doi.org/10.3389/fmicb.2023.1137869
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author Wang, Li
Chen, Junyu
He, Lin
Liu, Hanbo
Liu, Yan
Luan, Zonghui
Li, Hong
Liu, Weixin
Luo, Mengjun
author_facet Wang, Li
Chen, Junyu
He, Lin
Liu, Hanbo
Liu, Yan
Luan, Zonghui
Li, Hong
Liu, Weixin
Luo, Mengjun
author_sort Wang, Li
collection PubMed
description The aim of this study was to explore the microecological distribution and differences in the uterus and vaginal microbiome in women with early embryonic arrest and those with normal pregnancy by high-throughput sequencing. We systematically sampled the vaginal and uterine microbiomes of 56 pregnant women, namely, 38 patients with early embryonic arrest and 18 pregnant women with normal pregnancy-induced abortion. We obtained colonization data by 16S rRNA gene amplicon sequencing. In the vagina, Lactobacillus, Bacteroidetes and Helicobacter exhibited significant differences between the groups. We further found that Lactobacillus iners, Lactobacillus crispatus, Lactobacillus gasseri and Lactobacillus jensenii were the most dominant Lactobacillus species and that L. iners was significantly different between the groups. Receiver operating characteristic (ROC) curve analysis confirmed that Ensifer had the highest predictive value for early embryonic arrest. In the uterine cavity, we determined that Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were the dominant bacteria at the phylum level and that Bacteroides, Pseudarthrobacter, Lactobacillus and Ralstonia were the dominant genera. Further classification of Lactobacillus revealed that L. iners, L. crispatus, L. gasseri, and L. jensenii were the main species. There was a significant difference in L. jensenii between the normal pregnancy group and early embryonic arrest group. Random forest analysis revealed 18 different genera in the uterus, and ROC curve analysis indicated that Candidatus Symbiobacter, Odoribacter, Blautia, Nocardioides and Ileibacterium had a certain predictive value.
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spelling pubmed-100735712023-04-06 Association between the vaginal and uterine microbiota and the risk of early embryonic arrest Wang, Li Chen, Junyu He, Lin Liu, Hanbo Liu, Yan Luan, Zonghui Li, Hong Liu, Weixin Luo, Mengjun Front Microbiol Microbiology The aim of this study was to explore the microecological distribution and differences in the uterus and vaginal microbiome in women with early embryonic arrest and those with normal pregnancy by high-throughput sequencing. We systematically sampled the vaginal and uterine microbiomes of 56 pregnant women, namely, 38 patients with early embryonic arrest and 18 pregnant women with normal pregnancy-induced abortion. We obtained colonization data by 16S rRNA gene amplicon sequencing. In the vagina, Lactobacillus, Bacteroidetes and Helicobacter exhibited significant differences between the groups. We further found that Lactobacillus iners, Lactobacillus crispatus, Lactobacillus gasseri and Lactobacillus jensenii were the most dominant Lactobacillus species and that L. iners was significantly different between the groups. Receiver operating characteristic (ROC) curve analysis confirmed that Ensifer had the highest predictive value for early embryonic arrest. In the uterine cavity, we determined that Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were the dominant bacteria at the phylum level and that Bacteroides, Pseudarthrobacter, Lactobacillus and Ralstonia were the dominant genera. Further classification of Lactobacillus revealed that L. iners, L. crispatus, L. gasseri, and L. jensenii were the main species. There was a significant difference in L. jensenii between the normal pregnancy group and early embryonic arrest group. Random forest analysis revealed 18 different genera in the uterus, and ROC curve analysis indicated that Candidatus Symbiobacter, Odoribacter, Blautia, Nocardioides and Ileibacterium had a certain predictive value. Frontiers Media S.A. 2023-03-22 /pmc/articles/PMC10073571/ /pubmed/37032888 http://dx.doi.org/10.3389/fmicb.2023.1137869 Text en Copyright © 2023 Wang, Chen, He, Liu, Liu, Luan, Li, Liu and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Li
Chen, Junyu
He, Lin
Liu, Hanbo
Liu, Yan
Luan, Zonghui
Li, Hong
Liu, Weixin
Luo, Mengjun
Association between the vaginal and uterine microbiota and the risk of early embryonic arrest
title Association between the vaginal and uterine microbiota and the risk of early embryonic arrest
title_full Association between the vaginal and uterine microbiota and the risk of early embryonic arrest
title_fullStr Association between the vaginal and uterine microbiota and the risk of early embryonic arrest
title_full_unstemmed Association between the vaginal and uterine microbiota and the risk of early embryonic arrest
title_short Association between the vaginal and uterine microbiota and the risk of early embryonic arrest
title_sort association between the vaginal and uterine microbiota and the risk of early embryonic arrest
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073571/
https://www.ncbi.nlm.nih.gov/pubmed/37032888
http://dx.doi.org/10.3389/fmicb.2023.1137869
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