Cargando…

Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study

Purpose: To evaluate the association of platelet (PL) mitochondria respiration with markers of cardiovascular health in children ages 7–10 years. Methods: PL mitochondrial respiration (n = 91) was assessed by high resolution respirometry (HRR): Routine (R) respiration, complex (C) I linked respirati...

Descripción completa

Detalles Bibliográficos
Autores principales: Diaz, Eva C., Adams, Sean H., Weber, Judith L., Cotter, Matthew, Børsheim, Elisabet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073692/
https://www.ncbi.nlm.nih.gov/pubmed/37033448
http://dx.doi.org/10.3389/fmolb.2023.1136975
_version_ 1785019625007742976
author Diaz, Eva C.
Adams, Sean H.
Weber, Judith L.
Cotter, Matthew
Børsheim, Elisabet
author_facet Diaz, Eva C.
Adams, Sean H.
Weber, Judith L.
Cotter, Matthew
Børsheim, Elisabet
author_sort Diaz, Eva C.
collection PubMed
description Purpose: To evaluate the association of platelet (PL) mitochondria respiration with markers of cardiovascular health in children ages 7–10 years. Methods: PL mitochondrial respiration (n = 91) was assessed by high resolution respirometry (HRR): Routine (R) respiration, complex (C) I linked respiration (CI), and maximal uncoupled electron transport capacity of CII (CII(E)) were measured. The respiratory control ratio (RCR) was calculated as the ratio of maximal oxidative phosphorylation capacity of CI and CI leak respiration (P(CI)/L(CI)). Peak [Formula: see text] O(2) (incremental bike test) and body composition (dual-energy X-ray absorptiometry) were measured. Multiple generalized linear regression analysis was used to model the association of measures by HRR with variables of interest: adiposity, low-density lipoprotein (LDL-C) and triglyceride (TG) status (normal vs. elevated) HOMA2-IR, blood pressure status (normal vs. high), and demographics. Results: R and CI-linked respiration positively associated with adiposity, high blood pressure (HBP), and peak [Formula: see text] O(2). R and CI-linked respiration had inverse association with age and elevated LDL-C. CII(E) was higher in children with elevated LDL-C (log-β = −0.54, p = 0.010). HBP and peak [Formula: see text] O(2) interacted in relation to RCR (log-β = −0.01, p = 0.028). Specifically, RCR was lowest among children with HBP and low aerobic capacity (i.e., mean peak [Formula: see text] O(2) -1SD). HOMA2-IR did not associate with measures of PL mitochondria respiration. Conclusion: In PL, R and CI-linked mitochondrial respiration directly associate with adiposity, peak [Formula: see text] O(2) and HBP. Elevated LDL-C associates with lower CI-linked respiration which is compensated by increasing CII respiration. PL bioenergetics phenotypes in children associate with whole-body metabolic health status.
format Online
Article
Text
id pubmed-10073692
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100736922023-04-06 Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study Diaz, Eva C. Adams, Sean H. Weber, Judith L. Cotter, Matthew Børsheim, Elisabet Front Mol Biosci Molecular Biosciences Purpose: To evaluate the association of platelet (PL) mitochondria respiration with markers of cardiovascular health in children ages 7–10 years. Methods: PL mitochondrial respiration (n = 91) was assessed by high resolution respirometry (HRR): Routine (R) respiration, complex (C) I linked respiration (CI), and maximal uncoupled electron transport capacity of CII (CII(E)) were measured. The respiratory control ratio (RCR) was calculated as the ratio of maximal oxidative phosphorylation capacity of CI and CI leak respiration (P(CI)/L(CI)). Peak [Formula: see text] O(2) (incremental bike test) and body composition (dual-energy X-ray absorptiometry) were measured. Multiple generalized linear regression analysis was used to model the association of measures by HRR with variables of interest: adiposity, low-density lipoprotein (LDL-C) and triglyceride (TG) status (normal vs. elevated) HOMA2-IR, blood pressure status (normal vs. high), and demographics. Results: R and CI-linked respiration positively associated with adiposity, high blood pressure (HBP), and peak [Formula: see text] O(2). R and CI-linked respiration had inverse association with age and elevated LDL-C. CII(E) was higher in children with elevated LDL-C (log-β = −0.54, p = 0.010). HBP and peak [Formula: see text] O(2) interacted in relation to RCR (log-β = −0.01, p = 0.028). Specifically, RCR was lowest among children with HBP and low aerobic capacity (i.e., mean peak [Formula: see text] O(2) -1SD). HOMA2-IR did not associate with measures of PL mitochondria respiration. Conclusion: In PL, R and CI-linked mitochondrial respiration directly associate with adiposity, peak [Formula: see text] O(2) and HBP. Elevated LDL-C associates with lower CI-linked respiration which is compensated by increasing CII respiration. PL bioenergetics phenotypes in children associate with whole-body metabolic health status. Frontiers Media S.A. 2023-03-22 /pmc/articles/PMC10073692/ /pubmed/37033448 http://dx.doi.org/10.3389/fmolb.2023.1136975 Text en Copyright © 2023 Diaz, Adams, Weber, Cotter and Børsheim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Diaz, Eva C.
Adams, Sean H.
Weber, Judith L.
Cotter, Matthew
Børsheim, Elisabet
Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study
title Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study
title_full Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study
title_fullStr Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study
title_full_unstemmed Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study
title_short Elevated LDL-C, high blood pressure, and low peak [Formula: see text] O(2) associate with platelet mitochondria function in children—The Arkansas Active Kids Study
title_sort elevated ldl-c, high blood pressure, and low peak [formula: see text] o(2) associate with platelet mitochondria function in children—the arkansas active kids study
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10073692/
https://www.ncbi.nlm.nih.gov/pubmed/37033448
http://dx.doi.org/10.3389/fmolb.2023.1136975
work_keys_str_mv AT diazevac elevatedldlchighbloodpressureandlowpeakformulaseetexto2associatewithplateletmitochondriafunctioninchildrenthearkansasactivekidsstudy
AT adamsseanh elevatedldlchighbloodpressureandlowpeakformulaseetexto2associatewithplateletmitochondriafunctioninchildrenthearkansasactivekidsstudy
AT weberjudithl elevatedldlchighbloodpressureandlowpeakformulaseetexto2associatewithplateletmitochondriafunctioninchildrenthearkansasactivekidsstudy
AT cottermatthew elevatedldlchighbloodpressureandlowpeakformulaseetexto2associatewithplateletmitochondriafunctioninchildrenthearkansasactivekidsstudy
AT børsheimelisabet elevatedldlchighbloodpressureandlowpeakformulaseetexto2associatewithplateletmitochondriafunctioninchildrenthearkansasactivekidsstudy