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Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress

INTRODUCTION: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, as...

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Autores principales: Vukašinović, Aleksandra, Ostanek, Barbara, Klisic, Aleksandra, Kafedžić, Srdjan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Memon, Lidija, Gaković, Branka, Bogavac-Stanojević, Nataša, Spasojević-Kalimanovska, Vesna, Marc, Janja, Nešković, Aleksandar N., Kotur-Stevuljević, Jelena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074292/
https://www.ncbi.nlm.nih.gov/pubmed/37034531
http://dx.doi.org/10.5114/aoms/136074
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author Vukašinović, Aleksandra
Ostanek, Barbara
Klisic, Aleksandra
Kafedžić, Srdjan
Zdravković, Marija
Ilić, Ivan
Sopić, Miron
Hinić, Saša
Stefanović, Milica
Memon, Lidija
Gaković, Branka
Bogavac-Stanojević, Nataša
Spasojević-Kalimanovska, Vesna
Marc, Janja
Nešković, Aleksandar N.
Kotur-Stevuljević, Jelena
author_facet Vukašinović, Aleksandra
Ostanek, Barbara
Klisic, Aleksandra
Kafedžić, Srdjan
Zdravković, Marija
Ilić, Ivan
Sopić, Miron
Hinić, Saša
Stefanović, Milica
Memon, Lidija
Gaković, Branka
Bogavac-Stanojević, Nataša
Spasojević-Kalimanovska, Vesna
Marc, Janja
Nešković, Aleksandar N.
Kotur-Stevuljević, Jelena
author_sort Vukašinović, Aleksandra
collection PubMed
description INTRODUCTION: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. MATERIAL AND METHODS: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. RESULTS: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. CONCLUSIONS: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.
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spelling pubmed-100742922023-04-06 Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress Vukašinović, Aleksandra Ostanek, Barbara Klisic, Aleksandra Kafedžić, Srdjan Zdravković, Marija Ilić, Ivan Sopić, Miron Hinić, Saša Stefanović, Milica Memon, Lidija Gaković, Branka Bogavac-Stanojević, Nataša Spasojević-Kalimanovska, Vesna Marc, Janja Nešković, Aleksandar N. Kotur-Stevuljević, Jelena Arch Med Sci Clinical Research INTRODUCTION: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. MATERIAL AND METHODS: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. RESULTS: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. CONCLUSIONS: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress. Termedia Publishing House 2021-04-30 /pmc/articles/PMC10074292/ /pubmed/37034531 http://dx.doi.org/10.5114/aoms/136074 Text en Copyright: © 2021 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Clinical Research
Vukašinović, Aleksandra
Ostanek, Barbara
Klisic, Aleksandra
Kafedžić, Srdjan
Zdravković, Marija
Ilić, Ivan
Sopić, Miron
Hinić, Saša
Stefanović, Milica
Memon, Lidija
Gaković, Branka
Bogavac-Stanojević, Nataša
Spasojević-Kalimanovska, Vesna
Marc, Janja
Nešković, Aleksandar N.
Kotur-Stevuljević, Jelena
Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
title Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
title_full Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
title_fullStr Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
title_full_unstemmed Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
title_short Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
title_sort telomere-telomerase system status in patients with acute myocardial infarction with st-segment elevation – relationship with oxidative stress
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074292/
https://www.ncbi.nlm.nih.gov/pubmed/37034531
http://dx.doi.org/10.5114/aoms/136074
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