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Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins
BACKGROUND: Some peptides are targets for degradation when heterologously expressed as fusion proteins in E. coli, which can limit yields after isolation and purification. We recently reported that peptide degradation may be prevented by production of a “sandwiched” SUMO-peptide-intein (SPI) fusion...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074672/ https://www.ncbi.nlm.nih.gov/pubmed/37020212 http://dx.doi.org/10.1186/s12896-023-00779-5 |