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Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida

Pasteurella multocida can infect a multitude of wild and domesticated animals, with infections in cattle resulting in hemorrhagic septicemia (HS) or contributing to bovine respiratory disease (BRD) complex. Current cattle vaccines against P. multocida consist of inactivated bacteria, which only offe...

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Autores principales: Islam, Epshita A., Fegan, Jamie E., Tefera, Takele A., Curran, David M., Waeckerlin, Regula C., Ng, Dixon, Ahn, Sang Kyun, Lai, Chun Heng Royce, Nguyen, Quynh Huong, Shah, Megha, Tesfaw, Liyuwork, Adamu, Kassaye, Medhin, Wubet W., Legesse, Abinet, Deresse, Getaw, Getachew, Belayneh, Rawlyk, Neil, Evans, Brock, Potter, Andrew, Schryvers, Anthony B., Gray-Owen, Scott D., Moraes, Trevor F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10075479/
https://www.ncbi.nlm.nih.gov/pubmed/36961851
http://dx.doi.org/10.1371/journal.ppat.1011249
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author Islam, Epshita A.
Fegan, Jamie E.
Tefera, Takele A.
Curran, David M.
Waeckerlin, Regula C.
Ng, Dixon
Ahn, Sang Kyun
Lai, Chun Heng Royce
Nguyen, Quynh Huong
Shah, Megha
Tesfaw, Liyuwork
Adamu, Kassaye
Medhin, Wubet W.
Legesse, Abinet
Deresse, Getaw
Getachew, Belayneh
Rawlyk, Neil
Evans, Brock
Potter, Andrew
Schryvers, Anthony B.
Gray-Owen, Scott D.
Moraes, Trevor F.
author_facet Islam, Epshita A.
Fegan, Jamie E.
Tefera, Takele A.
Curran, David M.
Waeckerlin, Regula C.
Ng, Dixon
Ahn, Sang Kyun
Lai, Chun Heng Royce
Nguyen, Quynh Huong
Shah, Megha
Tesfaw, Liyuwork
Adamu, Kassaye
Medhin, Wubet W.
Legesse, Abinet
Deresse, Getaw
Getachew, Belayneh
Rawlyk, Neil
Evans, Brock
Potter, Andrew
Schryvers, Anthony B.
Gray-Owen, Scott D.
Moraes, Trevor F.
author_sort Islam, Epshita A.
collection PubMed
description Pasteurella multocida can infect a multitude of wild and domesticated animals, with infections in cattle resulting in hemorrhagic septicemia (HS) or contributing to bovine respiratory disease (BRD) complex. Current cattle vaccines against P. multocida consist of inactivated bacteria, which only offer limited and serogroup specific protection. Here, we describe a newly identified surface lipoprotein, PmSLP, that is present in nearly all annotated P. multocida strains isolated from cattle. Bovine associated variants span three of the four identified phylogenetic clusters, with PmSLP-1 and PmSLP-2 being restricted to BRD associated isolates and PmSLP-3 being restricted to isolates associated with HS. Recombinantly expressed, soluble PmSLP-1 (BRD-PmSLP) and PmSLP-3 (HS-PmSLP) vaccines were both able to provide full protection in a mouse sepsis model against the matched P. multocida strain, however no cross-protection and minimal serum IgG cross-reactivity was identified. Full protection against both challenge strains was achieved with a bivalent vaccine containing both BRD-PmSLP and HS-PmSLP, with serum IgG from immunized mice being highly reactive to both variants. Year-long stability studies with lyophilized antigen stored under various temperatures show no appreciable difference in biophysical properties or loss of efficacy in the mouse challenge model. PmSLP-1 and PmSLP-3 vaccines were each evaluated for immunogenicity in two independent cattle trials involving animals of different age ranges and breeds. In all four trials, vaccination with PmSLP resulted in an increase in antigen specific serum IgG over baseline. In a blinded cattle challenge study with a recently isolated HS strain, the matched HS-PmSLP vaccine showed strong efficacy (75–87.5% survival compared to 0% in the control group). Together, these data suggest that cattle vaccines composed of PmSLP antigens can be a practical and effective solution for preventing HS and BRD related P. multocida infections.
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spelling pubmed-100754792023-04-06 Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida Islam, Epshita A. Fegan, Jamie E. Tefera, Takele A. Curran, David M. Waeckerlin, Regula C. Ng, Dixon Ahn, Sang Kyun Lai, Chun Heng Royce Nguyen, Quynh Huong Shah, Megha Tesfaw, Liyuwork Adamu, Kassaye Medhin, Wubet W. Legesse, Abinet Deresse, Getaw Getachew, Belayneh Rawlyk, Neil Evans, Brock Potter, Andrew Schryvers, Anthony B. Gray-Owen, Scott D. Moraes, Trevor F. PLoS Pathog Research Article Pasteurella multocida can infect a multitude of wild and domesticated animals, with infections in cattle resulting in hemorrhagic septicemia (HS) or contributing to bovine respiratory disease (BRD) complex. Current cattle vaccines against P. multocida consist of inactivated bacteria, which only offer limited and serogroup specific protection. Here, we describe a newly identified surface lipoprotein, PmSLP, that is present in nearly all annotated P. multocida strains isolated from cattle. Bovine associated variants span three of the four identified phylogenetic clusters, with PmSLP-1 and PmSLP-2 being restricted to BRD associated isolates and PmSLP-3 being restricted to isolates associated with HS. Recombinantly expressed, soluble PmSLP-1 (BRD-PmSLP) and PmSLP-3 (HS-PmSLP) vaccines were both able to provide full protection in a mouse sepsis model against the matched P. multocida strain, however no cross-protection and minimal serum IgG cross-reactivity was identified. Full protection against both challenge strains was achieved with a bivalent vaccine containing both BRD-PmSLP and HS-PmSLP, with serum IgG from immunized mice being highly reactive to both variants. Year-long stability studies with lyophilized antigen stored under various temperatures show no appreciable difference in biophysical properties or loss of efficacy in the mouse challenge model. PmSLP-1 and PmSLP-3 vaccines were each evaluated for immunogenicity in two independent cattle trials involving animals of different age ranges and breeds. In all four trials, vaccination with PmSLP resulted in an increase in antigen specific serum IgG over baseline. In a blinded cattle challenge study with a recently isolated HS strain, the matched HS-PmSLP vaccine showed strong efficacy (75–87.5% survival compared to 0% in the control group). Together, these data suggest that cattle vaccines composed of PmSLP antigens can be a practical and effective solution for preventing HS and BRD related P. multocida infections. Public Library of Science 2023-03-24 /pmc/articles/PMC10075479/ /pubmed/36961851 http://dx.doi.org/10.1371/journal.ppat.1011249 Text en © 2023 Islam et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Islam, Epshita A.
Fegan, Jamie E.
Tefera, Takele A.
Curran, David M.
Waeckerlin, Regula C.
Ng, Dixon
Ahn, Sang Kyun
Lai, Chun Heng Royce
Nguyen, Quynh Huong
Shah, Megha
Tesfaw, Liyuwork
Adamu, Kassaye
Medhin, Wubet W.
Legesse, Abinet
Deresse, Getaw
Getachew, Belayneh
Rawlyk, Neil
Evans, Brock
Potter, Andrew
Schryvers, Anthony B.
Gray-Owen, Scott D.
Moraes, Trevor F.
Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida
title Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida
title_full Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida
title_fullStr Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida
title_full_unstemmed Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida
title_short Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida
title_sort reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by pasteurella multocida
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10075479/
https://www.ncbi.nlm.nih.gov/pubmed/36961851
http://dx.doi.org/10.1371/journal.ppat.1011249
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