Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling

Chemotherapy mistreatment is partially due to a lack of rapid and reliable tools to discriminate between sensitive and resistant phenotypes. In many cases, the resistance mechanism is not fully understood, contributing to the diagnostic tools' absence. This work aims to determine the capacity o...

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Autores principales: Ledesma, Martín, Poodts, Daniela, Amoia, Sofía, Hajos, Silvia, Fundia, Ariela, Vay, Carlos, Pibuel, Matías, Lompardía, Silvina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076308/
https://www.ncbi.nlm.nih.gov/pubmed/37019937
http://dx.doi.org/10.1038/s41598-023-32608-2
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author Ledesma, Martín
Poodts, Daniela
Amoia, Sofía
Hajos, Silvia
Fundia, Ariela
Vay, Carlos
Pibuel, Matías
Lompardía, Silvina
author_facet Ledesma, Martín
Poodts, Daniela
Amoia, Sofía
Hajos, Silvia
Fundia, Ariela
Vay, Carlos
Pibuel, Matías
Lompardía, Silvina
author_sort Ledesma, Martín
collection PubMed
description Chemotherapy mistreatment is partially due to a lack of rapid and reliable tools to discriminate between sensitive and resistant phenotypes. In many cases, the resistance mechanism is not fully understood, contributing to the diagnostic tools' absence. This work aims to determine the capacity of MALDI-TOF-MS profiling to discriminate between chemotherapy-resistant and sensitive phenotypes in leukemia and glioblastoma cells. A multivariate analysis of two therapy-resistant leukemia cell lines (Ki562 and Kv562) and two TMZ-resistant glioblastoma cell lines (U251-R and LN229-R) and their sensitive counterparts was performed. In this work, we first show MALDI-TOF-MS patterns analysis ability to differentiate these cancer cell lines by their chemotherapy-resistant status. We present a rapid and inexpensive tool that would guide and complement the therapeutic decision.
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spelling pubmed-100763082023-04-07 Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling Ledesma, Martín Poodts, Daniela Amoia, Sofía Hajos, Silvia Fundia, Ariela Vay, Carlos Pibuel, Matías Lompardía, Silvina Sci Rep Article Chemotherapy mistreatment is partially due to a lack of rapid and reliable tools to discriminate between sensitive and resistant phenotypes. In many cases, the resistance mechanism is not fully understood, contributing to the diagnostic tools' absence. This work aims to determine the capacity of MALDI-TOF-MS profiling to discriminate between chemotherapy-resistant and sensitive phenotypes in leukemia and glioblastoma cells. A multivariate analysis of two therapy-resistant leukemia cell lines (Ki562 and Kv562) and two TMZ-resistant glioblastoma cell lines (U251-R and LN229-R) and their sensitive counterparts was performed. In this work, we first show MALDI-TOF-MS patterns analysis ability to differentiate these cancer cell lines by their chemotherapy-resistant status. We present a rapid and inexpensive tool that would guide and complement the therapeutic decision. Nature Publishing Group UK 2023-04-05 /pmc/articles/PMC10076308/ /pubmed/37019937 http://dx.doi.org/10.1038/s41598-023-32608-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ledesma, Martín
Poodts, Daniela
Amoia, Sofía
Hajos, Silvia
Fundia, Ariela
Vay, Carlos
Pibuel, Matías
Lompardía, Silvina
Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling
title Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling
title_full Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling
title_fullStr Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling
title_full_unstemmed Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling
title_short Discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by MALDI-TOF-MS profiling
title_sort discrimination of the chemotherapy resistance status of human leukemia and glioblastoma cell lines by maldi-tof-ms profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076308/
https://www.ncbi.nlm.nih.gov/pubmed/37019937
http://dx.doi.org/10.1038/s41598-023-32608-2
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