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The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds
Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076396/ https://www.ncbi.nlm.nih.gov/pubmed/36719514 http://dx.doi.org/10.1007/s13353-023-00748-w |
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author | Androsiuk, Piotr Milarska, Sylwia Eryka Dulska, Justyna Kellmann-Sopyła, Wioleta Szablińska-Piernik, Joanna Lahuta, Lesław Bernard |
author_facet | Androsiuk, Piotr Milarska, Sylwia Eryka Dulska, Justyna Kellmann-Sopyła, Wioleta Szablińska-Piernik, Joanna Lahuta, Lesław Bernard |
author_sort | Androsiuk, Piotr |
collection | PubMed |
description | Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and expected heterozygosity (H(e)) revealed low genetic diversity, with the highest values observed for A. nuda (I = 0.099; He = 0.068). UPGMA clustering of studied Avena accessions and PCoA results showed that the polyploidy level is the main grouping criterion. High-resolution gas chromatography revealed that the studied Avena accessions share the same composition of soluble carbohydrates, but significant differences in the content of total (5.30–22.38 mg g(−1) of dry weight) and particular sugars among studied samples were observed. Sucrose appeared as the most abundant sugar (mean 61.52% of total soluble carbohydrates), followed by raffinose family oligosaccharides (31.23%), myo-inositol and its galactosides (6.16%), and monosaccharides (1.09%). The pattern of interspecific variation in soluble carbohydrates, showed by PCA, was convergent to that revealed by iPBS markers. Thus, both methods appeared as a source of valuable data useful in the characterization of Avena resources or in the discussion on the evolution of this genus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13353-023-00748-w. |
format | Online Article Text |
id | pubmed-10076396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-100763962023-04-07 The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds Androsiuk, Piotr Milarska, Sylwia Eryka Dulska, Justyna Kellmann-Sopyła, Wioleta Szablińska-Piernik, Joanna Lahuta, Lesław Bernard J Appl Genet Plant Genetics • Original Paper Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and expected heterozygosity (H(e)) revealed low genetic diversity, with the highest values observed for A. nuda (I = 0.099; He = 0.068). UPGMA clustering of studied Avena accessions and PCoA results showed that the polyploidy level is the main grouping criterion. High-resolution gas chromatography revealed that the studied Avena accessions share the same composition of soluble carbohydrates, but significant differences in the content of total (5.30–22.38 mg g(−1) of dry weight) and particular sugars among studied samples were observed. Sucrose appeared as the most abundant sugar (mean 61.52% of total soluble carbohydrates), followed by raffinose family oligosaccharides (31.23%), myo-inositol and its galactosides (6.16%), and monosaccharides (1.09%). The pattern of interspecific variation in soluble carbohydrates, showed by PCA, was convergent to that revealed by iPBS markers. Thus, both methods appeared as a source of valuable data useful in the characterization of Avena resources or in the discussion on the evolution of this genus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13353-023-00748-w. Springer Berlin Heidelberg 2023-01-31 2023 /pmc/articles/PMC10076396/ /pubmed/36719514 http://dx.doi.org/10.1007/s13353-023-00748-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Plant Genetics • Original Paper Androsiuk, Piotr Milarska, Sylwia Eryka Dulska, Justyna Kellmann-Sopyła, Wioleta Szablińska-Piernik, Joanna Lahuta, Lesław Bernard The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds |
title | The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds |
title_full | The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds |
title_fullStr | The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds |
title_full_unstemmed | The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds |
title_short | The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds |
title_sort | comparison of polymorphism among avena species revealed by retrotransposon-based dna markers and soluble carbohydrates in seeds |
topic | Plant Genetics • Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076396/ https://www.ncbi.nlm.nih.gov/pubmed/36719514 http://dx.doi.org/10.1007/s13353-023-00748-w |
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