Cargando…

YB1 participated in regulating mitochondrial activity through RNA replacement

As a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not been fully elucidated. Here, our work has found that Y-bo...

Descripción completa

Detalles Bibliográficos
Autores principales: Gong, Weipeng, Zhang, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076880/
https://www.ncbi.nlm.nih.gov/pubmed/37035211
http://dx.doi.org/10.3389/fonc.2023.1145379
_version_ 1785020233752248320
author Gong, Weipeng
Zhang, Song
author_facet Gong, Weipeng
Zhang, Song
author_sort Gong, Weipeng
collection PubMed
description As a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not been fully elucidated. Here, our work has found that Y-box binding protein 1 (YB1) maintained cellular autophagy at a moderate level to inhibit mitochondrial oxidative phosphorylation. In addition, mitochondrial RNA was leaked into cytosol under starvation, accompanied by YB1 mitochondrial relocation, resulting in YB1-bound RNA replacement. The mRNAs encoded by oxidative phosphorylation (OXPHOS)-associated genes and oncogene HMGA1 (high-mobility group AT-hook 1) were competitively replaced by mitochondria-derived tRNAs. The increase of free OXPHOS mRNAs released from the YB1 complex enhanced mitochondrial activity through facilitating translation, but the stability of HMGA1 mRNA was impaired without the protection of YB1, both contributing to breast cancer cell apoptosis and reactive oxygen species production. Our finding not only provided a new potential target for breast cancer therapy but also shed new light on understanding the global landscape of cellular interactions between RNA-binding proteins and different RNA species.
format Online
Article
Text
id pubmed-10076880
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100768802023-04-07 YB1 participated in regulating mitochondrial activity through RNA replacement Gong, Weipeng Zhang, Song Front Oncol Oncology As a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not been fully elucidated. Here, our work has found that Y-box binding protein 1 (YB1) maintained cellular autophagy at a moderate level to inhibit mitochondrial oxidative phosphorylation. In addition, mitochondrial RNA was leaked into cytosol under starvation, accompanied by YB1 mitochondrial relocation, resulting in YB1-bound RNA replacement. The mRNAs encoded by oxidative phosphorylation (OXPHOS)-associated genes and oncogene HMGA1 (high-mobility group AT-hook 1) were competitively replaced by mitochondria-derived tRNAs. The increase of free OXPHOS mRNAs released from the YB1 complex enhanced mitochondrial activity through facilitating translation, but the stability of HMGA1 mRNA was impaired without the protection of YB1, both contributing to breast cancer cell apoptosis and reactive oxygen species production. Our finding not only provided a new potential target for breast cancer therapy but also shed new light on understanding the global landscape of cellular interactions between RNA-binding proteins and different RNA species. Frontiers Media S.A. 2023-03-23 /pmc/articles/PMC10076880/ /pubmed/37035211 http://dx.doi.org/10.3389/fonc.2023.1145379 Text en Copyright © 2023 Gong and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Gong, Weipeng
Zhang, Song
YB1 participated in regulating mitochondrial activity through RNA replacement
title YB1 participated in regulating mitochondrial activity through RNA replacement
title_full YB1 participated in regulating mitochondrial activity through RNA replacement
title_fullStr YB1 participated in regulating mitochondrial activity through RNA replacement
title_full_unstemmed YB1 participated in regulating mitochondrial activity through RNA replacement
title_short YB1 participated in regulating mitochondrial activity through RNA replacement
title_sort yb1 participated in regulating mitochondrial activity through rna replacement
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076880/
https://www.ncbi.nlm.nih.gov/pubmed/37035211
http://dx.doi.org/10.3389/fonc.2023.1145379
work_keys_str_mv AT gongweipeng yb1participatedinregulatingmitochondrialactivitythroughrnareplacement
AT zhangsong yb1participatedinregulatingmitochondrialactivitythroughrnareplacement