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YB1 participated in regulating mitochondrial activity through RNA replacement
As a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not been fully elucidated. Here, our work has found that Y-bo...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076880/ https://www.ncbi.nlm.nih.gov/pubmed/37035211 http://dx.doi.org/10.3389/fonc.2023.1145379 |
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author | Gong, Weipeng Zhang, Song |
author_facet | Gong, Weipeng Zhang, Song |
author_sort | Gong, Weipeng |
collection | PubMed |
description | As a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not been fully elucidated. Here, our work has found that Y-box binding protein 1 (YB1) maintained cellular autophagy at a moderate level to inhibit mitochondrial oxidative phosphorylation. In addition, mitochondrial RNA was leaked into cytosol under starvation, accompanied by YB1 mitochondrial relocation, resulting in YB1-bound RNA replacement. The mRNAs encoded by oxidative phosphorylation (OXPHOS)-associated genes and oncogene HMGA1 (high-mobility group AT-hook 1) were competitively replaced by mitochondria-derived tRNAs. The increase of free OXPHOS mRNAs released from the YB1 complex enhanced mitochondrial activity through facilitating translation, but the stability of HMGA1 mRNA was impaired without the protection of YB1, both contributing to breast cancer cell apoptosis and reactive oxygen species production. Our finding not only provided a new potential target for breast cancer therapy but also shed new light on understanding the global landscape of cellular interactions between RNA-binding proteins and different RNA species. |
format | Online Article Text |
id | pubmed-10076880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100768802023-04-07 YB1 participated in regulating mitochondrial activity through RNA replacement Gong, Weipeng Zhang, Song Front Oncol Oncology As a relic of ancient bacterial endosymbionts, mitochondria play a central role in cell metabolism, apoptosis, autophagy, and other processes. However, the function of mitochondria-derived nucleic acids in cellular signal transduction has not been fully elucidated. Here, our work has found that Y-box binding protein 1 (YB1) maintained cellular autophagy at a moderate level to inhibit mitochondrial oxidative phosphorylation. In addition, mitochondrial RNA was leaked into cytosol under starvation, accompanied by YB1 mitochondrial relocation, resulting in YB1-bound RNA replacement. The mRNAs encoded by oxidative phosphorylation (OXPHOS)-associated genes and oncogene HMGA1 (high-mobility group AT-hook 1) were competitively replaced by mitochondria-derived tRNAs. The increase of free OXPHOS mRNAs released from the YB1 complex enhanced mitochondrial activity through facilitating translation, but the stability of HMGA1 mRNA was impaired without the protection of YB1, both contributing to breast cancer cell apoptosis and reactive oxygen species production. Our finding not only provided a new potential target for breast cancer therapy but also shed new light on understanding the global landscape of cellular interactions between RNA-binding proteins and different RNA species. Frontiers Media S.A. 2023-03-23 /pmc/articles/PMC10076880/ /pubmed/37035211 http://dx.doi.org/10.3389/fonc.2023.1145379 Text en Copyright © 2023 Gong and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Gong, Weipeng Zhang, Song YB1 participated in regulating mitochondrial activity through RNA replacement |
title | YB1 participated in regulating mitochondrial activity through RNA replacement |
title_full | YB1 participated in regulating mitochondrial activity through RNA replacement |
title_fullStr | YB1 participated in regulating mitochondrial activity through RNA replacement |
title_full_unstemmed | YB1 participated in regulating mitochondrial activity through RNA replacement |
title_short | YB1 participated in regulating mitochondrial activity through RNA replacement |
title_sort | yb1 participated in regulating mitochondrial activity through rna replacement |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076880/ https://www.ncbi.nlm.nih.gov/pubmed/37035211 http://dx.doi.org/10.3389/fonc.2023.1145379 |
work_keys_str_mv | AT gongweipeng yb1participatedinregulatingmitochondrialactivitythroughrnareplacement AT zhangsong yb1participatedinregulatingmitochondrialactivitythroughrnareplacement |