Cargando…

Microencapsulation of Probiotic Streptococcus salivarius LAB813

[Image: see text] Probiotics are living microorganisms that confer a health benefit on the host when administered in adequate amounts. Streptococcus salivarius, a commensal bacterium found in the oral cavity, has been shown to secrete antimicrobial peptides and can be used as probiotics. This study...

Descripción completa

Detalles Bibliográficos
Autores principales: Choudhary, Priyadarshani, Kraatz, Heinz-Bernhard, Lévesque, Céline M., Gong, Siew-Ging
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077535/
https://www.ncbi.nlm.nih.gov/pubmed/37033842
http://dx.doi.org/10.1021/acsomega.2c07721
_version_ 1785020322480652288
author Choudhary, Priyadarshani
Kraatz, Heinz-Bernhard
Lévesque, Céline M.
Gong, Siew-Ging
author_facet Choudhary, Priyadarshani
Kraatz, Heinz-Bernhard
Lévesque, Céline M.
Gong, Siew-Ging
author_sort Choudhary, Priyadarshani
collection PubMed
description [Image: see text] Probiotics are living microorganisms that confer a health benefit on the host when administered in adequate amounts. Streptococcus salivarius, a commensal bacterium found in the oral cavity, has been shown to secrete antimicrobial peptides and can be used as probiotics. This study aimed to develop a delivery system for the probiotic LAB813, a novel S. salivarius strain first identified in the laboratory. Probiotics can be delivered and protected through the encapsulation of biomaterials such as polysaccharides. Their biocompatibility, biodegradability, user-friendliness, and ease of access make polysaccharides useful for encapsulating probiotics. Alginate (Alg) and chitosan (Ch) are naturally obtained polysaccharides and, hence, tested for LAB813 encapsulation. An extrusion method of encapsulation was performed to form Alg microcapsules (Alg-LAB813), some of which were coated with Ch (Alg-LAB813-Ch) to provide dual-layered protection. Inhibitory assays of the Alg-LAB813 and Alg-LAB813-Ch microcapsules were assayed against an indicator strain. Alg-LAB813-Ch microcapsules showed superior antibacterial properties compared to Alg-LAB813 microcapsules over 24 h and when subject to temperatures ranging from 4 to 68 °C. In addition, Alg-LAB813-Ch microcapsules retained antibacterial activity for up to 28 days of storage at 4 °C. The strong and sustained inhibitory activities of Ch-coated Alg encapsulated LAB813 signify the potential for their use to improve oral health.
format Online
Article
Text
id pubmed-10077535
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-100775352023-04-07 Microencapsulation of Probiotic Streptococcus salivarius LAB813 Choudhary, Priyadarshani Kraatz, Heinz-Bernhard Lévesque, Céline M. Gong, Siew-Ging ACS Omega [Image: see text] Probiotics are living microorganisms that confer a health benefit on the host when administered in adequate amounts. Streptococcus salivarius, a commensal bacterium found in the oral cavity, has been shown to secrete antimicrobial peptides and can be used as probiotics. This study aimed to develop a delivery system for the probiotic LAB813, a novel S. salivarius strain first identified in the laboratory. Probiotics can be delivered and protected through the encapsulation of biomaterials such as polysaccharides. Their biocompatibility, biodegradability, user-friendliness, and ease of access make polysaccharides useful for encapsulating probiotics. Alginate (Alg) and chitosan (Ch) are naturally obtained polysaccharides and, hence, tested for LAB813 encapsulation. An extrusion method of encapsulation was performed to form Alg microcapsules (Alg-LAB813), some of which were coated with Ch (Alg-LAB813-Ch) to provide dual-layered protection. Inhibitory assays of the Alg-LAB813 and Alg-LAB813-Ch microcapsules were assayed against an indicator strain. Alg-LAB813-Ch microcapsules showed superior antibacterial properties compared to Alg-LAB813 microcapsules over 24 h and when subject to temperatures ranging from 4 to 68 °C. In addition, Alg-LAB813-Ch microcapsules retained antibacterial activity for up to 28 days of storage at 4 °C. The strong and sustained inhibitory activities of Ch-coated Alg encapsulated LAB813 signify the potential for their use to improve oral health. American Chemical Society 2023-03-23 /pmc/articles/PMC10077535/ /pubmed/37033842 http://dx.doi.org/10.1021/acsomega.2c07721 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Choudhary, Priyadarshani
Kraatz, Heinz-Bernhard
Lévesque, Céline M.
Gong, Siew-Ging
Microencapsulation of Probiotic Streptococcus salivarius LAB813
title Microencapsulation of Probiotic Streptococcus salivarius LAB813
title_full Microencapsulation of Probiotic Streptococcus salivarius LAB813
title_fullStr Microencapsulation of Probiotic Streptococcus salivarius LAB813
title_full_unstemmed Microencapsulation of Probiotic Streptococcus salivarius LAB813
title_short Microencapsulation of Probiotic Streptococcus salivarius LAB813
title_sort microencapsulation of probiotic streptococcus salivarius lab813
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077535/
https://www.ncbi.nlm.nih.gov/pubmed/37033842
http://dx.doi.org/10.1021/acsomega.2c07721
work_keys_str_mv AT choudharypriyadarshani microencapsulationofprobioticstreptococcussalivariuslab813
AT kraatzheinzbernhard microencapsulationofprobioticstreptococcussalivariuslab813
AT levesquecelinem microencapsulationofprobioticstreptococcussalivariuslab813
AT gongsiewging microencapsulationofprobioticstreptococcussalivariuslab813