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Temporal Proteomic and Lipidomic Profiles of Cerulein-Induced Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations in the Disease Pathogenesis
[Image: see text] The pathophysiological mechanisms of acute pancreatitis (AP) are complex and have remained a mystery to date, but metabolism is gradually recognized as an important driver of AP onset and development. We used a cerulein-induced AP mouse model to conduct liquid chromatography–mass s...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077560/ https://www.ncbi.nlm.nih.gov/pubmed/37033809 http://dx.doi.org/10.1021/acsomega.3c00019 |
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author | Wang, Rui Wang, Yiqin Tao, Yiran Hu, Liqiang Qiu, Qi Pu, Qianlun Yang, Juqin Wang, Shisheng Huang, Yan Chen, Xiaoting Zhu, Ping Yang, Hao Xia, Qing Du, Dan |
author_facet | Wang, Rui Wang, Yiqin Tao, Yiran Hu, Liqiang Qiu, Qi Pu, Qianlun Yang, Juqin Wang, Shisheng Huang, Yan Chen, Xiaoting Zhu, Ping Yang, Hao Xia, Qing Du, Dan |
author_sort | Wang, Rui |
collection | PubMed |
description | [Image: see text] The pathophysiological mechanisms of acute pancreatitis (AP) are complex and have remained a mystery to date, but metabolism is gradually recognized as an important driver of AP onset and development. We used a cerulein-induced AP mouse model to conduct liquid chromatography–mass spectrometry (LC–MS/MS)-based time-course proteomics and lipidomics in order to better understand the underlying metabolic alterations linked with AP. Results showed that a series of significant changes in proteins over time with a boost in expression were enriched in lipase activity, lipoprotein, and lipids absorption and transport regulation. Furthermore, 16 proteins associated with lipid metabolism and signaling pathways together with the whole lipid species changing profile led to the vital identification of changing law in glycerides, phosphoglycerides, and free fatty acids. In addition to lipid metabolism and regulation-associated proteins, several digestive enzymes and adaptive anti-trypsin, stress response, and energy metabolism-related proteins showed an increment in abundance. Notably, central carbon and branched chain amino acid metabolism were enhanced during 0–24 h from the first cerulein stimulation. Taken together, this integrated proteomics and lipidomics revealed a novel metabolic insight into metabolites transforming rules that might be relevant to their function and drug targets investigation. (Created with Biorender.com.) |
format | Online Article Text |
id | pubmed-10077560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-100775602023-04-07 Temporal Proteomic and Lipidomic Profiles of Cerulein-Induced Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations in the Disease Pathogenesis Wang, Rui Wang, Yiqin Tao, Yiran Hu, Liqiang Qiu, Qi Pu, Qianlun Yang, Juqin Wang, Shisheng Huang, Yan Chen, Xiaoting Zhu, Ping Yang, Hao Xia, Qing Du, Dan ACS Omega [Image: see text] The pathophysiological mechanisms of acute pancreatitis (AP) are complex and have remained a mystery to date, but metabolism is gradually recognized as an important driver of AP onset and development. We used a cerulein-induced AP mouse model to conduct liquid chromatography–mass spectrometry (LC–MS/MS)-based time-course proteomics and lipidomics in order to better understand the underlying metabolic alterations linked with AP. Results showed that a series of significant changes in proteins over time with a boost in expression were enriched in lipase activity, lipoprotein, and lipids absorption and transport regulation. Furthermore, 16 proteins associated with lipid metabolism and signaling pathways together with the whole lipid species changing profile led to the vital identification of changing law in glycerides, phosphoglycerides, and free fatty acids. In addition to lipid metabolism and regulation-associated proteins, several digestive enzymes and adaptive anti-trypsin, stress response, and energy metabolism-related proteins showed an increment in abundance. Notably, central carbon and branched chain amino acid metabolism were enhanced during 0–24 h from the first cerulein stimulation. Taken together, this integrated proteomics and lipidomics revealed a novel metabolic insight into metabolites transforming rules that might be relevant to their function and drug targets investigation. (Created with Biorender.com.) American Chemical Society 2023-03-21 /pmc/articles/PMC10077560/ /pubmed/37033809 http://dx.doi.org/10.1021/acsomega.3c00019 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Wang, Rui Wang, Yiqin Tao, Yiran Hu, Liqiang Qiu, Qi Pu, Qianlun Yang, Juqin Wang, Shisheng Huang, Yan Chen, Xiaoting Zhu, Ping Yang, Hao Xia, Qing Du, Dan Temporal Proteomic and Lipidomic Profiles of Cerulein-Induced Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations in the Disease Pathogenesis |
title | Temporal Proteomic
and Lipidomic Profiles of Cerulein-Induced
Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations
in the Disease Pathogenesis |
title_full | Temporal Proteomic
and Lipidomic Profiles of Cerulein-Induced
Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations
in the Disease Pathogenesis |
title_fullStr | Temporal Proteomic
and Lipidomic Profiles of Cerulein-Induced
Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations
in the Disease Pathogenesis |
title_full_unstemmed | Temporal Proteomic
and Lipidomic Profiles of Cerulein-Induced
Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations
in the Disease Pathogenesis |
title_short | Temporal Proteomic
and Lipidomic Profiles of Cerulein-Induced
Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations
in the Disease Pathogenesis |
title_sort | temporal proteomic
and lipidomic profiles of cerulein-induced
acute pancreatitis reveal novel insights for metabolic alterations
in the disease pathogenesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077560/ https://www.ncbi.nlm.nih.gov/pubmed/37033809 http://dx.doi.org/10.1021/acsomega.3c00019 |
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