FMRP binds Per1 mRNA and downregulates its protein expression in mice

FMRP, an RNA-binding protein, has previously shown to be involved in regulation of circadian rhythms in flies and mice. However, the molecular mechanism remains elusive. Here we demonstrate that core circadian component Per1 mRNA was a target of FMRP and the association leads to reduced PER1 express...

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Autores principales: Tang, Xiangrong, Zhang, Jing, Li, Xin, Hu, Ying, Liu, Dengfeng, Li, Jia-Da, Lu, Renbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077598/
https://www.ncbi.nlm.nih.gov/pubmed/37020302
http://dx.doi.org/10.1186/s13041-023-01023-z
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author Tang, Xiangrong
Zhang, Jing
Li, Xin
Hu, Ying
Liu, Dengfeng
Li, Jia-Da
Lu, Renbin
author_facet Tang, Xiangrong
Zhang, Jing
Li, Xin
Hu, Ying
Liu, Dengfeng
Li, Jia-Da
Lu, Renbin
author_sort Tang, Xiangrong
collection PubMed
description FMRP, an RNA-binding protein, has previously shown to be involved in regulation of circadian rhythms in flies and mice. However, the molecular mechanism remains elusive. Here we demonstrate that core circadian component Per1 mRNA was a target of FMRP and the association leads to reduced PER1 expression. In Fmr1 KO mice, the oscillation of PER1 protein expression was significantly affected in a temporal and tissue-dependent pattern when compared to WT mice. Our work thus identified Per1 mRNA as a novel target of FMRP and suggested a potential role of FMRP in regulation of circadian function. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13041-023-01023-z.
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spelling pubmed-100775982023-04-07 FMRP binds Per1 mRNA and downregulates its protein expression in mice Tang, Xiangrong Zhang, Jing Li, Xin Hu, Ying Liu, Dengfeng Li, Jia-Da Lu, Renbin Mol Brain Research FMRP, an RNA-binding protein, has previously shown to be involved in regulation of circadian rhythms in flies and mice. However, the molecular mechanism remains elusive. Here we demonstrate that core circadian component Per1 mRNA was a target of FMRP and the association leads to reduced PER1 expression. In Fmr1 KO mice, the oscillation of PER1 protein expression was significantly affected in a temporal and tissue-dependent pattern when compared to WT mice. Our work thus identified Per1 mRNA as a novel target of FMRP and suggested a potential role of FMRP in regulation of circadian function. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13041-023-01023-z. BioMed Central 2023-04-05 /pmc/articles/PMC10077598/ /pubmed/37020302 http://dx.doi.org/10.1186/s13041-023-01023-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Tang, Xiangrong
Zhang, Jing
Li, Xin
Hu, Ying
Liu, Dengfeng
Li, Jia-Da
Lu, Renbin
FMRP binds Per1 mRNA and downregulates its protein expression in mice
title FMRP binds Per1 mRNA and downregulates its protein expression in mice
title_full FMRP binds Per1 mRNA and downregulates its protein expression in mice
title_fullStr FMRP binds Per1 mRNA and downregulates its protein expression in mice
title_full_unstemmed FMRP binds Per1 mRNA and downregulates its protein expression in mice
title_short FMRP binds Per1 mRNA and downregulates its protein expression in mice
title_sort fmrp binds per1 mrna and downregulates its protein expression in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077598/
https://www.ncbi.nlm.nih.gov/pubmed/37020302
http://dx.doi.org/10.1186/s13041-023-01023-z
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