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Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078256/ https://www.ncbi.nlm.nih.gov/pubmed/35898167 http://dx.doi.org/10.1002/wnan.1835 |
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author | De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel Malhador |
author_facet | De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel Malhador |
author_sort | De Sousa, Karina P. |
collection | PubMed |
description | Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV‐related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. This article is categorized under: Diagnostic Tools > Biosensing. Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease. Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease. |
format | Online Article Text |
id | pubmed-10078256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100782562023-04-07 Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel Malhador Wiley Interdiscip Rev Nanomed Nanobiotechnol Advanced Reviews Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV‐related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. This article is categorized under: Diagnostic Tools > Biosensing. Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease. Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease. John Wiley & Sons, Inc. 2022-07-27 2023 /pmc/articles/PMC10078256/ /pubmed/35898167 http://dx.doi.org/10.1002/wnan.1835 Text en © 2022 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Advanced Reviews De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel Malhador Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
title | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
title_full | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
title_fullStr | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
title_full_unstemmed | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
title_short | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
title_sort | isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
topic | Advanced Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078256/ https://www.ncbi.nlm.nih.gov/pubmed/35898167 http://dx.doi.org/10.1002/wnan.1835 |
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