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Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi
BACKGROUND: Streptococcus equi subspecies equi (S equi) is the cause of Strangles, one of the most prevalent diseases of horses worldwide. Variation within the immunodominant SeM protein has been documented, but a new eight‐component fusion protein vaccine, Strangvac, does not contain live S equi or...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078666/ https://www.ncbi.nlm.nih.gov/pubmed/35000217 http://dx.doi.org/10.1111/evj.13552 |
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author | Frosth, Sara Morris, Ellen Ruth A. Wilson, Hayley Frykberg, Lars Jacobsson, Karin Parkhill, Julian Flock, Jan‐Ingmar Wood, Tim Guss, Bengt Aanensen, David M. Boyle, Ashley G. Riihimäki, Miia Cohen, Noah D. Waller, Andrew S. |
author_facet | Frosth, Sara Morris, Ellen Ruth A. Wilson, Hayley Frykberg, Lars Jacobsson, Karin Parkhill, Julian Flock, Jan‐Ingmar Wood, Tim Guss, Bengt Aanensen, David M. Boyle, Ashley G. Riihimäki, Miia Cohen, Noah D. Waller, Andrew S. |
author_sort | Frosth, Sara |
collection | PubMed |
description | BACKGROUND: Streptococcus equi subspecies equi (S equi) is the cause of Strangles, one of the most prevalent diseases of horses worldwide. Variation within the immunodominant SeM protein has been documented, but a new eight‐component fusion protein vaccine, Strangvac, does not contain live S equi or SeM and conservation of the antigens it contains have not been reported. OBJECTIVE: To define the diversity of the eight Strangvac antigens across a diverse S equi population. STUDY DESIGN: Genomic description. METHODS: Antigen sequences from the genomes of 759 S equi isolates from 19 countries, recovered between 1955 and 2018, were analysed. Predicted amino acid sequences in the antigen fragments of SEQ0256(Eq5), SEQ0402(Eq8), SEQ0721(EAG), SEQ0855(SclF), SEQ0935(CNE), SEQ0999(IdeE), SEQ1817(SclI) and SEQ2101(SclC) in Strangvac and SeM were extracted from the 759 assembled genomes and compared. RESULTS: The predicted amino acid sequences of SclC, SclI and IdeE were identical across all 759 genomes. CNE was truncated in the genome of five (0.7%) isolates. SclF was absent from one genome and another encoded a single amino acid substitution. EAG was truncated in two genomes. Eq5 was truncated in four genomes and 123 genomes encoded a single amino acid substitution. Eq8 was truncated in three genomes, one genome encoded four amino acid substitutions and 398 genomes encoded a single amino acid substitution at the final amino acid of the Eq8 antigen fragment. Therefore, at least 1579 (99.9%) of 1580 amino acids in Strangvac were identical in 743 (97.9%) genomes, and all genomes encoded identical amino acid sequences for at least six of the eight Strangvac antigens. MAIN LIMITATIONS: Three hundred and seven (40.4%) isolates in this study were recovered from horses in the UK. CONCLUSIONS: The predicted amino acid sequences of antigens in Strangvac were highly conserved across this collection of S equi. |
format | Online Article Text |
id | pubmed-10078666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100786662023-04-07 Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi Frosth, Sara Morris, Ellen Ruth A. Wilson, Hayley Frykberg, Lars Jacobsson, Karin Parkhill, Julian Flock, Jan‐Ingmar Wood, Tim Guss, Bengt Aanensen, David M. Boyle, Ashley G. Riihimäki, Miia Cohen, Noah D. Waller, Andrew S. Equine Vet J Experimental and Basic Research Studies BACKGROUND: Streptococcus equi subspecies equi (S equi) is the cause of Strangles, one of the most prevalent diseases of horses worldwide. Variation within the immunodominant SeM protein has been documented, but a new eight‐component fusion protein vaccine, Strangvac, does not contain live S equi or SeM and conservation of the antigens it contains have not been reported. OBJECTIVE: To define the diversity of the eight Strangvac antigens across a diverse S equi population. STUDY DESIGN: Genomic description. METHODS: Antigen sequences from the genomes of 759 S equi isolates from 19 countries, recovered between 1955 and 2018, were analysed. Predicted amino acid sequences in the antigen fragments of SEQ0256(Eq5), SEQ0402(Eq8), SEQ0721(EAG), SEQ0855(SclF), SEQ0935(CNE), SEQ0999(IdeE), SEQ1817(SclI) and SEQ2101(SclC) in Strangvac and SeM were extracted from the 759 assembled genomes and compared. RESULTS: The predicted amino acid sequences of SclC, SclI and IdeE were identical across all 759 genomes. CNE was truncated in the genome of five (0.7%) isolates. SclF was absent from one genome and another encoded a single amino acid substitution. EAG was truncated in two genomes. Eq5 was truncated in four genomes and 123 genomes encoded a single amino acid substitution. Eq8 was truncated in three genomes, one genome encoded four amino acid substitutions and 398 genomes encoded a single amino acid substitution at the final amino acid of the Eq8 antigen fragment. Therefore, at least 1579 (99.9%) of 1580 amino acids in Strangvac were identical in 743 (97.9%) genomes, and all genomes encoded identical amino acid sequences for at least six of the eight Strangvac antigens. MAIN LIMITATIONS: Three hundred and seven (40.4%) isolates in this study were recovered from horses in the UK. CONCLUSIONS: The predicted amino acid sequences of antigens in Strangvac were highly conserved across this collection of S equi. John Wiley and Sons Inc. 2022-01-19 2023-01 /pmc/articles/PMC10078666/ /pubmed/35000217 http://dx.doi.org/10.1111/evj.13552 Text en © 2022 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Experimental and Basic Research Studies Frosth, Sara Morris, Ellen Ruth A. Wilson, Hayley Frykberg, Lars Jacobsson, Karin Parkhill, Julian Flock, Jan‐Ingmar Wood, Tim Guss, Bengt Aanensen, David M. Boyle, Ashley G. Riihimäki, Miia Cohen, Noah D. Waller, Andrew S. Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi |
title | Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi
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title_full | Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi
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title_fullStr | Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi
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title_full_unstemmed | Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi
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title_short | Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi
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title_sort | conservation of vaccine antigen sequences encoded by sequenced strains of streptococcus equi subsp. equi |
topic | Experimental and Basic Research Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078666/ https://www.ncbi.nlm.nih.gov/pubmed/35000217 http://dx.doi.org/10.1111/evj.13552 |
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