Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet

BACKGROUND: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre...

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Detalles Bibliográficos
Autores principales: van der Velden, Vincent H. J., Preijers, Frank, Johansson, Ulrika, Westers, Theresia M., Dunlop, Alan, Porwit, Anna, Béné, Marie C., Valent, Peter, te Marvelde, Jeroen, Wagner‐Ballon, Orianne, Oelschlaegel, Uta, Saft, Leonie, Kordasti, Sharham, Ireland, Robin, Cremers, Eline, Alhan, Canan, Duetz, Carolien, Hobo, Willemijn, Chapuis, Nicolas, Fontenay, Michaela, Bettelheim, Peter, Eidenshink‐Brodersen, Lisa, Font, Patricia, Loken, Michael R., Matarraz, Sergio, Ogata, Kiyoyuki, Orfao, Alberto, Psarra, Katherina, Subirá, Dolores, Wells, Denise A., Della Porta, Matteo G., Burbury, Kate, Bellos, Frauke, Weiß, Elisabeth, Kern, Wolfgang, van de Loosdrecht, Arjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078694/
https://www.ncbi.nlm.nih.gov/pubmed/34894176
http://dx.doi.org/10.1002/cyto.b.22046
Descripción
Sumario:BACKGROUND: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre‐analytical procedures. METHODS: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. RESULTS: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. CONCLUSIONS: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.

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