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Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
BACKGROUND: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078694/ https://www.ncbi.nlm.nih.gov/pubmed/34894176 http://dx.doi.org/10.1002/cyto.b.22046 |
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| author | van der Velden, Vincent H. J. Preijers, Frank Johansson, Ulrika Westers, Theresia M. Dunlop, Alan Porwit, Anna Béné, Marie C. Valent, Peter te Marvelde, Jeroen Wagner‐Ballon, Orianne Oelschlaegel, Uta Saft, Leonie Kordasti, Sharham Ireland, Robin Cremers, Eline Alhan, Canan Duetz, Carolien Hobo, Willemijn Chapuis, Nicolas Fontenay, Michaela Bettelheim, Peter Eidenshink‐Brodersen, Lisa Font, Patricia Loken, Michael R. Matarraz, Sergio Ogata, Kiyoyuki Orfao, Alberto Psarra, Katherina Subirá, Dolores Wells, Denise A. Della Porta, Matteo G. Burbury, Kate Bellos, Frauke Weiß, Elisabeth Kern, Wolfgang van de Loosdrecht, Arjan |
| author_facet | van der Velden, Vincent H. J. Preijers, Frank Johansson, Ulrika Westers, Theresia M. Dunlop, Alan Porwit, Anna Béné, Marie C. Valent, Peter te Marvelde, Jeroen Wagner‐Ballon, Orianne Oelschlaegel, Uta Saft, Leonie Kordasti, Sharham Ireland, Robin Cremers, Eline Alhan, Canan Duetz, Carolien Hobo, Willemijn Chapuis, Nicolas Fontenay, Michaela Bettelheim, Peter Eidenshink‐Brodersen, Lisa Font, Patricia Loken, Michael R. Matarraz, Sergio Ogata, Kiyoyuki Orfao, Alberto Psarra, Katherina Subirá, Dolores Wells, Denise A. Della Porta, Matteo G. Burbury, Kate Bellos, Frauke Weiß, Elisabeth Kern, Wolfgang van de Loosdrecht, Arjan |
| author_sort | van der Velden, Vincent H. J. |
| collection | PubMed |
| description | BACKGROUND: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre‐analytical procedures. METHODS: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. RESULTS: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. CONCLUSIONS: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice. |
| format | Online Article Text |
| id | pubmed-10078694 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | John Wiley & Sons, Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-100786942023-04-07 Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet van der Velden, Vincent H. J. Preijers, Frank Johansson, Ulrika Westers, Theresia M. Dunlop, Alan Porwit, Anna Béné, Marie C. Valent, Peter te Marvelde, Jeroen Wagner‐Ballon, Orianne Oelschlaegel, Uta Saft, Leonie Kordasti, Sharham Ireland, Robin Cremers, Eline Alhan, Canan Duetz, Carolien Hobo, Willemijn Chapuis, Nicolas Fontenay, Michaela Bettelheim, Peter Eidenshink‐Brodersen, Lisa Font, Patricia Loken, Michael R. Matarraz, Sergio Ogata, Kiyoyuki Orfao, Alberto Psarra, Katherina Subirá, Dolores Wells, Denise A. Della Porta, Matteo G. Burbury, Kate Bellos, Frauke Weiß, Elisabeth Kern, Wolfgang van de Loosdrecht, Arjan Cytometry B Clin Cytom Original Article BACKGROUND: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre‐analytical procedures. METHODS: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. RESULTS: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. CONCLUSIONS: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice. John Wiley & Sons, Inc. 2021-12-11 2023-01 /pmc/articles/PMC10078694/ /pubmed/34894176 http://dx.doi.org/10.1002/cyto.b.22046 Text en © 2021 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Original Article van der Velden, Vincent H. J. Preijers, Frank Johansson, Ulrika Westers, Theresia M. Dunlop, Alan Porwit, Anna Béné, Marie C. Valent, Peter te Marvelde, Jeroen Wagner‐Ballon, Orianne Oelschlaegel, Uta Saft, Leonie Kordasti, Sharham Ireland, Robin Cremers, Eline Alhan, Canan Duetz, Carolien Hobo, Willemijn Chapuis, Nicolas Fontenay, Michaela Bettelheim, Peter Eidenshink‐Brodersen, Lisa Font, Patricia Loken, Michael R. Matarraz, Sergio Ogata, Kiyoyuki Orfao, Alberto Psarra, Katherina Subirá, Dolores Wells, Denise A. Della Porta, Matteo G. Burbury, Kate Bellos, Frauke Weiß, Elisabeth Kern, Wolfgang van de Loosdrecht, Arjan Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet |
| title | Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
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| title_full | Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
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| title_fullStr | Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
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| title_full_unstemmed | Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
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| title_short | Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
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| title_sort | flow cytometric analysis of myelodysplasia: pre‐analytical and technical issues—recommendations from the european leukemianet |
| topic | Original Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078694/ https://www.ncbi.nlm.nih.gov/pubmed/34894176 http://dx.doi.org/10.1002/cyto.b.22046 |
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