A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes

Current guidelines recommend flow cytometric analysis as part of the diagnostic assessment of patients with cytopenia suspected for myelodysplastic syndrome. Herein we describe the complete work‐up of six cases using multimodal integrated diagnostics. Flow cytometry assessments are illustrated by pl...

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Autores principales: Westers, Theresia M., Saft, Leonie, van der Velden, Vincent H. J., te Marvelde, Jeroen G., Dunlop, Alan, Ireland, Robin, Valent, Peter, Porwit, Anna, Béné, Marie C., van de Loosdrecht, Arjan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078764/
https://www.ncbi.nlm.nih.gov/pubmed/35179296
http://dx.doi.org/10.1002/cyto.b.22061
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author Westers, Theresia M.
Saft, Leonie
van der Velden, Vincent H. J.
te Marvelde, Jeroen G.
Dunlop, Alan
Ireland, Robin
Valent, Peter
Porwit, Anna
Béné, Marie C.
van de Loosdrecht, Arjan A.
author_facet Westers, Theresia M.
Saft, Leonie
van der Velden, Vincent H. J.
te Marvelde, Jeroen G.
Dunlop, Alan
Ireland, Robin
Valent, Peter
Porwit, Anna
Béné, Marie C.
van de Loosdrecht, Arjan A.
author_sort Westers, Theresia M.
collection PubMed
description Current guidelines recommend flow cytometric analysis as part of the diagnostic assessment of patients with cytopenia suspected for myelodysplastic syndrome. Herein we describe the complete work‐up of six cases using multimodal integrated diagnostics. Flow cytometry assessments are illustrated by plots from conventional and more recent analysis tools. The cases demonstrate the added value of flow cytometry in case of hypocellular, poor quality, or ambiguous bone marrow cytomorphology. Moreover, they demonstrate how immunophenotyping results support clinical decision‐making in inconclusive and clinically ‘difficult’ cases.
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spelling pubmed-100787642023-04-07 A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes Westers, Theresia M. Saft, Leonie van der Velden, Vincent H. J. te Marvelde, Jeroen G. Dunlop, Alan Ireland, Robin Valent, Peter Porwit, Anna Béné, Marie C. van de Loosdrecht, Arjan A. Cytometry B Clin Cytom Original Articles Current guidelines recommend flow cytometric analysis as part of the diagnostic assessment of patients with cytopenia suspected for myelodysplastic syndrome. Herein we describe the complete work‐up of six cases using multimodal integrated diagnostics. Flow cytometry assessments are illustrated by plots from conventional and more recent analysis tools. The cases demonstrate the added value of flow cytometry in case of hypocellular, poor quality, or ambiguous bone marrow cytomorphology. Moreover, they demonstrate how immunophenotyping results support clinical decision‐making in inconclusive and clinically ‘difficult’ cases. John Wiley & Sons, Inc. 2022-02-18 2023-01 /pmc/articles/PMC10078764/ /pubmed/35179296 http://dx.doi.org/10.1002/cyto.b.22061 Text en © 2022 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Westers, Theresia M.
Saft, Leonie
van der Velden, Vincent H. J.
te Marvelde, Jeroen G.
Dunlop, Alan
Ireland, Robin
Valent, Peter
Porwit, Anna
Béné, Marie C.
van de Loosdrecht, Arjan A.
A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
title A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
title_full A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
title_fullStr A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
title_full_unstemmed A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
title_short A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
title_sort series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078764/
https://www.ncbi.nlm.nih.gov/pubmed/35179296
http://dx.doi.org/10.1002/cyto.b.22061
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