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Mitochondrial nanomotion measured by optical microscopy
Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078959/ https://www.ncbi.nlm.nih.gov/pubmed/37032884 http://dx.doi.org/10.3389/fmicb.2023.1133773 |
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author | Parmar, Priyanka Villalba, Maria Ines Horii Huber, Alexandre Seiji Kalauzi, Aleksandar Bartolić, Dragana Radotić, Ksenija Willaert, Ronnie Guy MacFabe, Derrick F. Kasas, Sandor |
author_facet | Parmar, Priyanka Villalba, Maria Ines Horii Huber, Alexandre Seiji Kalauzi, Aleksandar Bartolić, Dragana Radotić, Ksenija Willaert, Ronnie Guy MacFabe, Derrick F. Kasas, Sandor |
author_sort | Parmar, Priyanka |
collection | PubMed |
description | Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free. |
format | Online Article Text |
id | pubmed-10078959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100789592023-04-07 Mitochondrial nanomotion measured by optical microscopy Parmar, Priyanka Villalba, Maria Ines Horii Huber, Alexandre Seiji Kalauzi, Aleksandar Bartolić, Dragana Radotić, Ksenija Willaert, Ronnie Guy MacFabe, Derrick F. Kasas, Sandor Front Microbiol Microbiology Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free. Frontiers Media S.A. 2023-03-23 /pmc/articles/PMC10078959/ /pubmed/37032884 http://dx.doi.org/10.3389/fmicb.2023.1133773 Text en Copyright © 2023 Parmar, Villalba, Horii Huber, Kalauzi, Bartolić, Radotić, Willaert, MacFabe and Kasas. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Parmar, Priyanka Villalba, Maria Ines Horii Huber, Alexandre Seiji Kalauzi, Aleksandar Bartolić, Dragana Radotić, Ksenija Willaert, Ronnie Guy MacFabe, Derrick F. Kasas, Sandor Mitochondrial nanomotion measured by optical microscopy |
title | Mitochondrial nanomotion measured by optical microscopy |
title_full | Mitochondrial nanomotion measured by optical microscopy |
title_fullStr | Mitochondrial nanomotion measured by optical microscopy |
title_full_unstemmed | Mitochondrial nanomotion measured by optical microscopy |
title_short | Mitochondrial nanomotion measured by optical microscopy |
title_sort | mitochondrial nanomotion measured by optical microscopy |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078959/ https://www.ncbi.nlm.nih.gov/pubmed/37032884 http://dx.doi.org/10.3389/fmicb.2023.1133773 |
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