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Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells
Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin wi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079685/ https://www.ncbi.nlm.nih.gov/pubmed/37024514 http://dx.doi.org/10.1038/s41598-023-32126-1 |
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author | Fujino, Shoko Hamano, Sayuri Tomokiyo, Atsushi Sugiura, Risa Yamashita, Daiki Hasegawa, Daigaku Sugii, Hideki Fujii, Shinsuke Itoyama, Tomohiro Miyaji, Hirofumi Maeda, Hidefumi |
author_facet | Fujino, Shoko Hamano, Sayuri Tomokiyo, Atsushi Sugiura, Risa Yamashita, Daiki Hasegawa, Daigaku Sugii, Hideki Fujii, Shinsuke Itoyama, Tomohiro Miyaji, Hirofumi Maeda, Hidefumi |
author_sort | Fujino, Shoko |
collection | PubMed |
description | Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues. |
format | Online Article Text |
id | pubmed-10079685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-100796852023-04-08 Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells Fujino, Shoko Hamano, Sayuri Tomokiyo, Atsushi Sugiura, Risa Yamashita, Daiki Hasegawa, Daigaku Sugii, Hideki Fujii, Shinsuke Itoyama, Tomohiro Miyaji, Hirofumi Maeda, Hidefumi Sci Rep Article Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues. Nature Publishing Group UK 2023-04-06 /pmc/articles/PMC10079685/ /pubmed/37024514 http://dx.doi.org/10.1038/s41598-023-32126-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Fujino, Shoko Hamano, Sayuri Tomokiyo, Atsushi Sugiura, Risa Yamashita, Daiki Hasegawa, Daigaku Sugii, Hideki Fujii, Shinsuke Itoyama, Tomohiro Miyaji, Hirofumi Maeda, Hidefumi Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
title | Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
title_full | Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
title_fullStr | Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
title_full_unstemmed | Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
title_short | Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
title_sort | dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079685/ https://www.ncbi.nlm.nih.gov/pubmed/37024514 http://dx.doi.org/10.1038/s41598-023-32126-1 |
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