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Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins
An analytical methodology based on the combination of dispersive magnetic solid-phase extraction (DMSPE) and liquid chromatography-mass spectrometry (LC-MS) is proposed to explore the occurrence of 13 mycotoxins (aflatoxins B(1), G(1), B(2), and G(2); deoxynivalenol; T-2 toxin; ochratoxin A; HT-2 to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079724/ https://www.ncbi.nlm.nih.gov/pubmed/37022520 http://dx.doi.org/10.1007/s00604-023-05763-6 |
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author | García-Nicolás, María Arroyo-Manzanares, Natalia Campillo, Natalia Reyes-Palomo, Carolina Sanz-Fernández, Santos Fenoll, José Rodríguez-Estévez, Vicente Viñas, Pilar |
author_facet | García-Nicolás, María Arroyo-Manzanares, Natalia Campillo, Natalia Reyes-Palomo, Carolina Sanz-Fernández, Santos Fenoll, José Rodríguez-Estévez, Vicente Viñas, Pilar |
author_sort | García-Nicolás, María |
collection | PubMed |
description | An analytical methodology based on the combination of dispersive magnetic solid-phase extraction (DMSPE) and liquid chromatography-mass spectrometry (LC-MS) is proposed to explore the occurrence of 13 mycotoxins (aflatoxins B(1), G(1), B(2), and G(2); deoxynivalenol; T-2 toxin; ochratoxin A; HT-2 toxin; enniatins A, A(1), B, and B(2); and beauvericin) and their derivatives in natural grass samples. Magnetic microparticles (Fe(3)O(4)) coated with polypyrrole (PPy) polymer were used in DMSPE sample treatment as adsorbent phase, and Fourier-transform infrared spectroscopy, field emission scanning electron microscopy, and energy dispersive X-ray spectroscopy have been used for its characterization. The experimental parameters influencing the adsorption and desorption steps of DMSPE have been optimized. Method validation has been carried out obtaining limits of quantification between 0.07 and 92 μg kg(−1) corresponding to enniatin B or A(1) and DON, respectively. A total of 83 natural grass samples from 8 dehesa farms were analysed. Enniatin B was found in all the samples (0.29 to 488 μg kg(−1) concentration range) followed by enniatin B(1) (92.8% of the samples) with a 0.12–137 μg kg(−1) concentration range. Moreover, co-occurrence of mycotoxins was studied and between 2 and 5 mycotoxins appeared simultaneously in 97.6% of the samples. Distribution of the contamination according to natural grass location was also investigated. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00604-023-05763-6. |
format | Online Article Text |
id | pubmed-10079724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-100797242023-04-08 Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins García-Nicolás, María Arroyo-Manzanares, Natalia Campillo, Natalia Reyes-Palomo, Carolina Sanz-Fernández, Santos Fenoll, José Rodríguez-Estévez, Vicente Viñas, Pilar Mikrochim Acta Original Paper An analytical methodology based on the combination of dispersive magnetic solid-phase extraction (DMSPE) and liquid chromatography-mass spectrometry (LC-MS) is proposed to explore the occurrence of 13 mycotoxins (aflatoxins B(1), G(1), B(2), and G(2); deoxynivalenol; T-2 toxin; ochratoxin A; HT-2 toxin; enniatins A, A(1), B, and B(2); and beauvericin) and their derivatives in natural grass samples. Magnetic microparticles (Fe(3)O(4)) coated with polypyrrole (PPy) polymer were used in DMSPE sample treatment as adsorbent phase, and Fourier-transform infrared spectroscopy, field emission scanning electron microscopy, and energy dispersive X-ray spectroscopy have been used for its characterization. The experimental parameters influencing the adsorption and desorption steps of DMSPE have been optimized. Method validation has been carried out obtaining limits of quantification between 0.07 and 92 μg kg(−1) corresponding to enniatin B or A(1) and DON, respectively. A total of 83 natural grass samples from 8 dehesa farms were analysed. Enniatin B was found in all the samples (0.29 to 488 μg kg(−1) concentration range) followed by enniatin B(1) (92.8% of the samples) with a 0.12–137 μg kg(−1) concentration range. Moreover, co-occurrence of mycotoxins was studied and between 2 and 5 mycotoxins appeared simultaneously in 97.6% of the samples. Distribution of the contamination according to natural grass location was also investigated. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00604-023-05763-6. Springer Vienna 2023-04-06 2023 /pmc/articles/PMC10079724/ /pubmed/37022520 http://dx.doi.org/10.1007/s00604-023-05763-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Paper García-Nicolás, María Arroyo-Manzanares, Natalia Campillo, Natalia Reyes-Palomo, Carolina Sanz-Fernández, Santos Fenoll, José Rodríguez-Estévez, Vicente Viñas, Pilar Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
title | Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
title_full | Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
title_fullStr | Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
title_full_unstemmed | Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
title_short | Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
title_sort | use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079724/ https://www.ncbi.nlm.nih.gov/pubmed/37022520 http://dx.doi.org/10.1007/s00604-023-05763-6 |
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