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Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats

Many recent research projects have described typical chronic changes in the retinal vasculature for diverse neurovascular and neurodegenerative disorders such as stroke or Alzheimer's disease. Unlike cerebral vasculature, retinal blood vessels can be assessed non-invasively by retinal vessel an...

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Autores principales: Warner, Laura, Bach-Hagemann, Annika, Schmidt, Tobias P., Pinkernell, Sarah, Schubert, Gerrit A., Clusmann, Hans, Albanna, Walid, Lindauer, Ute, Conzen-Dilger, Catharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079937/
https://www.ncbi.nlm.nih.gov/pubmed/37033376
http://dx.doi.org/10.3389/fnmol.2023.1116841
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author Warner, Laura
Bach-Hagemann, Annika
Schmidt, Tobias P.
Pinkernell, Sarah
Schubert, Gerrit A.
Clusmann, Hans
Albanna, Walid
Lindauer, Ute
Conzen-Dilger, Catharina
author_facet Warner, Laura
Bach-Hagemann, Annika
Schmidt, Tobias P.
Pinkernell, Sarah
Schubert, Gerrit A.
Clusmann, Hans
Albanna, Walid
Lindauer, Ute
Conzen-Dilger, Catharina
author_sort Warner, Laura
collection PubMed
description Many recent research projects have described typical chronic changes in the retinal vasculature for diverse neurovascular and neurodegenerative disorders such as stroke or Alzheimer's disease. Unlike cerebral vasculature, retinal blood vessels can be assessed non-invasively by retinal vessel analysis. To date, there is only a little information about potential simultaneous reactions of retinal and cerebral vessels in acute neurovascular diseases. The field of applications of retinal assessment could significantly be widened if more information about potential correlations between those two vascular beds and the feasibility of non-invasive retinal vessel analysis in acute neurovascular disease were available. Here, we present our protocol for the simultaneous assessment of retinal and cerebral vessels in an acute setting in anesthetized rats using a non-invasive retinal vessel analyzer and a superficial tissue imaging system for laser speckle contrast analysis via a closed bone window. We describe the experimental set-up in detail, outline the pitfalls of repeated retinal vessel analyses in an experimental set-up of several hours, and address issues that arise from the simultaneous use of two different assessment tools. Finally, we demonstrate the robustness and variability of the reactivity of retinal vessels to hypercapnia at baseline as well as their reproducibility over time using two anesthetic protocols common for neurovascular research. In summary, the procedures described in this protocol allow us to directly compare retinal and cerebral vascular beds and help to substantiate the role of the retina as a “window to the brain.”
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spelling pubmed-100799372023-04-08 Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats Warner, Laura Bach-Hagemann, Annika Schmidt, Tobias P. Pinkernell, Sarah Schubert, Gerrit A. Clusmann, Hans Albanna, Walid Lindauer, Ute Conzen-Dilger, Catharina Front Mol Neurosci Molecular Neuroscience Many recent research projects have described typical chronic changes in the retinal vasculature for diverse neurovascular and neurodegenerative disorders such as stroke or Alzheimer's disease. Unlike cerebral vasculature, retinal blood vessels can be assessed non-invasively by retinal vessel analysis. To date, there is only a little information about potential simultaneous reactions of retinal and cerebral vessels in acute neurovascular diseases. The field of applications of retinal assessment could significantly be widened if more information about potential correlations between those two vascular beds and the feasibility of non-invasive retinal vessel analysis in acute neurovascular disease were available. Here, we present our protocol for the simultaneous assessment of retinal and cerebral vessels in an acute setting in anesthetized rats using a non-invasive retinal vessel analyzer and a superficial tissue imaging system for laser speckle contrast analysis via a closed bone window. We describe the experimental set-up in detail, outline the pitfalls of repeated retinal vessel analyses in an experimental set-up of several hours, and address issues that arise from the simultaneous use of two different assessment tools. Finally, we demonstrate the robustness and variability of the reactivity of retinal vessels to hypercapnia at baseline as well as their reproducibility over time using two anesthetic protocols common for neurovascular research. In summary, the procedures described in this protocol allow us to directly compare retinal and cerebral vascular beds and help to substantiate the role of the retina as a “window to the brain.” Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10079937/ /pubmed/37033376 http://dx.doi.org/10.3389/fnmol.2023.1116841 Text en Copyright © 2023 Warner, Bach-Hagemann, Schmidt, Pinkernell, Schubert, Clusmann, Albanna, Lindauer and Conzen-Dilger. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Warner, Laura
Bach-Hagemann, Annika
Schmidt, Tobias P.
Pinkernell, Sarah
Schubert, Gerrit A.
Clusmann, Hans
Albanna, Walid
Lindauer, Ute
Conzen-Dilger, Catharina
Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
title Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
title_full Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
title_fullStr Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
title_full_unstemmed Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
title_short Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
title_sort opening a window to the acutely injured brain: simultaneous retinal and cerebral vascular monitoring in rats
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079937/
https://www.ncbi.nlm.nih.gov/pubmed/37033376
http://dx.doi.org/10.3389/fnmol.2023.1116841
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