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The identification and characterization of a plant height and grain length related gene hfr131 in rice

Plant height and grain size are important agronomic traits affecting rice yield. Various plant hormones participate in the regulation of plant height and grain size in rice. However, how these hormones cooperate to regulate plant height and grain size is poorly understood. In this study, we identifi...

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Autores principales: Lan, Dengyong, Cao, Liming, Liu, Mingyu, Ma, Fuying, Yan, Peiwen, Zhang, Xinwei, Hu, Jian, Niu, Fuan, He, Shicong, Cui, Jinhao, Yuan, Xinyu, Yang, Jinshui, Wang, Ying, Luo, Xiaojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080003/
https://www.ncbi.nlm.nih.gov/pubmed/37035088
http://dx.doi.org/10.3389/fpls.2023.1152196
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author Lan, Dengyong
Cao, Liming
Liu, Mingyu
Ma, Fuying
Yan, Peiwen
Zhang, Xinwei
Hu, Jian
Niu, Fuan
He, Shicong
Cui, Jinhao
Yuan, Xinyu
Yang, Jinshui
Wang, Ying
Luo, Xiaojin
author_facet Lan, Dengyong
Cao, Liming
Liu, Mingyu
Ma, Fuying
Yan, Peiwen
Zhang, Xinwei
Hu, Jian
Niu, Fuan
He, Shicong
Cui, Jinhao
Yuan, Xinyu
Yang, Jinshui
Wang, Ying
Luo, Xiaojin
author_sort Lan, Dengyong
collection PubMed
description Plant height and grain size are important agronomic traits affecting rice yield. Various plant hormones participate in the regulation of plant height and grain size in rice. However, how these hormones cooperate to regulate plant height and grain size is poorly understood. In this study, we identified a brassinosteroid-related gene, hfr131, from an introgression line constructed using Oryza longistaminata, that caused brassinosteroid insensitivity and reduced plant height and grain length in rice. Further study showed that hfr131 is a new allele of OsBRI1 with a single-nucleotide polymorphism (G to A) in the coding region, leading to a T988I conversion at a conserved site of the kinase domain. By combining yeast one-hybrid assays, chromatin immunoprecipitation-quantitative PCR and gene expression quantification, we demonstrated that OsARF17, an auxin response factor, could bind to the promoter region of HFR131 and positively regulated HFR131 expression, thereby regulating the plant height and grain length, and influencing brassinosteroid sensitivity. Haplotype analysis showed that the consociation of OsAFR17(Hap1) /HFR131(Hap6) conferred an increase in grain length. Overall, this study identified hfr131 as a new allele of OsBRI1 that regulates plant height and grain length in rice, revealed that brassinosteroid and auxin might coordinate through OsARF17–HFR131 interaction, and provided a potential breeding target for improvement of rice yield.
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spelling pubmed-100800032023-04-08 The identification and characterization of a plant height and grain length related gene hfr131 in rice Lan, Dengyong Cao, Liming Liu, Mingyu Ma, Fuying Yan, Peiwen Zhang, Xinwei Hu, Jian Niu, Fuan He, Shicong Cui, Jinhao Yuan, Xinyu Yang, Jinshui Wang, Ying Luo, Xiaojin Front Plant Sci Plant Science Plant height and grain size are important agronomic traits affecting rice yield. Various plant hormones participate in the regulation of plant height and grain size in rice. However, how these hormones cooperate to regulate plant height and grain size is poorly understood. In this study, we identified a brassinosteroid-related gene, hfr131, from an introgression line constructed using Oryza longistaminata, that caused brassinosteroid insensitivity and reduced plant height and grain length in rice. Further study showed that hfr131 is a new allele of OsBRI1 with a single-nucleotide polymorphism (G to A) in the coding region, leading to a T988I conversion at a conserved site of the kinase domain. By combining yeast one-hybrid assays, chromatin immunoprecipitation-quantitative PCR and gene expression quantification, we demonstrated that OsARF17, an auxin response factor, could bind to the promoter region of HFR131 and positively regulated HFR131 expression, thereby regulating the plant height and grain length, and influencing brassinosteroid sensitivity. Haplotype analysis showed that the consociation of OsAFR17(Hap1) /HFR131(Hap6) conferred an increase in grain length. Overall, this study identified hfr131 as a new allele of OsBRI1 that regulates plant height and grain length in rice, revealed that brassinosteroid and auxin might coordinate through OsARF17–HFR131 interaction, and provided a potential breeding target for improvement of rice yield. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10080003/ /pubmed/37035088 http://dx.doi.org/10.3389/fpls.2023.1152196 Text en Copyright © 2023 Lan, Cao, Liu, Ma, Yan, Zhang, Hu, Niu, He, Cui, Yuan, Yang, Wang and Luo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lan, Dengyong
Cao, Liming
Liu, Mingyu
Ma, Fuying
Yan, Peiwen
Zhang, Xinwei
Hu, Jian
Niu, Fuan
He, Shicong
Cui, Jinhao
Yuan, Xinyu
Yang, Jinshui
Wang, Ying
Luo, Xiaojin
The identification and characterization of a plant height and grain length related gene hfr131 in rice
title The identification and characterization of a plant height and grain length related gene hfr131 in rice
title_full The identification and characterization of a plant height and grain length related gene hfr131 in rice
title_fullStr The identification and characterization of a plant height and grain length related gene hfr131 in rice
title_full_unstemmed The identification and characterization of a plant height and grain length related gene hfr131 in rice
title_short The identification and characterization of a plant height and grain length related gene hfr131 in rice
title_sort identification and characterization of a plant height and grain length related gene hfr131 in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080003/
https://www.ncbi.nlm.nih.gov/pubmed/37035088
http://dx.doi.org/10.3389/fpls.2023.1152196
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