Cargando…
Long-term simulated microgravity alters gut microbiota and metabolome in mice
Spaceflight and microgravity has a significant impact on the immune, central nervous, bone, and muscle support and cardiovascular systems. However, limited studies are available on the adverse effects of long-term microgravity on the intestinal microbiota, metabolism, and its relationships. In this...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080065/ https://www.ncbi.nlm.nih.gov/pubmed/37032862 http://dx.doi.org/10.3389/fmicb.2023.1100747 |
_version_ | 1785020843979440128 |
---|---|
author | Yuan, Lu Zhang, Rong Li, Xinlou Gao, Caiyun Hu, Xiangnan Hussain, Safdar Zhang, Linlin Wang, Moye Ma, Xiaoyu Pan, Qiuxia Lou, Xiaotong Si, Shaoyan |
author_facet | Yuan, Lu Zhang, Rong Li, Xinlou Gao, Caiyun Hu, Xiangnan Hussain, Safdar Zhang, Linlin Wang, Moye Ma, Xiaoyu Pan, Qiuxia Lou, Xiaotong Si, Shaoyan |
author_sort | Yuan, Lu |
collection | PubMed |
description | Spaceflight and microgravity has a significant impact on the immune, central nervous, bone, and muscle support and cardiovascular systems. However, limited studies are available on the adverse effects of long-term microgravity on the intestinal microbiota, metabolism, and its relationships. In this study, a ground-based simulated microgravity (SMG) mouse model was established to evaluate the impact of long-term microgravity on gut microbiota and metabolome. After 8 weeks of SMG, alterations of the intestinal microbiota and metabolites were detected using 16S rRNA sequencing and untargeted metabolomics. Compared to the control, no significant differences in α-diversity were observed at weeks 2, 4 and 8. Nevertheless, there were clear differences in community structures at different time points. The phylum Verrucomicrobia significantly declined from 2 to 8 weeks of SMG, yet the relative abundance of Actinobacteria and Deferribacteres expanded remarkably at weeks 8. SMG decreased the genus of Allobaculum and increased Bacteroides significantly throughout the period of 8 weeks. Besides, Genus Akkermansia, Gracilibacter, Prevotella, Odoribacter, Rothia, Sporosarcina, Gracilibacter, Clostridium, and Mucispirillum were identified as biomarkers for SMG group. Desulfovibrio_c21_c20, Akkermansia_muciniphila, and Ruminococcus_gnavus dropped at week 2, which tend to recover at week 4, except for Akkermansia_muciniphila. Bacteroides_uniformis and Faecalibacterium_prausnitzii declined significantly, while Ruminococcus_flavefaciens and Mucispirillum_schaedleri elevated at week 8. Furthermore, intestinal metabolome analysis showed that 129 were upregulated and 146 metabolites were downregulated in SMG. Long-term SMG most affected steroid hormone biosynthesis, tryptophan, cysteine, methionine, arginine, proline metabolism, and histidine metabolism. Correlated analysis suggested that the potential beneficial taxa Allobaculum, Akkermansia, and Faecalibacterium were negatively associated with tryptophan, histidine, arginine, and proline metabolism, but positively with steroid hormone biosynthesis. Yet Bacteroides, Lachnospiraceae_Clostridium, Rothia, Bilophila, and Coprococcus were positively correlated with arginine, proline, tryptophan, and histidine metabolism, while negatively associated with steroid hormone biosynthesis. These results suggest that Long-term SMG altered the community of intestinal microbiota, and then further disturbed intestinal metabolites and metabolic pathways, which have great potential to help understand and provide clues for revealing the mechanisms of long-term SMG involved diseases. |
format | Online Article Text |
id | pubmed-10080065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100800652023-04-08 Long-term simulated microgravity alters gut microbiota and metabolome in mice Yuan, Lu Zhang, Rong Li, Xinlou Gao, Caiyun Hu, Xiangnan Hussain, Safdar Zhang, Linlin Wang, Moye Ma, Xiaoyu Pan, Qiuxia Lou, Xiaotong Si, Shaoyan Front Microbiol Microbiology Spaceflight and microgravity has a significant impact on the immune, central nervous, bone, and muscle support and cardiovascular systems. However, limited studies are available on the adverse effects of long-term microgravity on the intestinal microbiota, metabolism, and its relationships. In this study, a ground-based simulated microgravity (SMG) mouse model was established to evaluate the impact of long-term microgravity on gut microbiota and metabolome. After 8 weeks of SMG, alterations of the intestinal microbiota and metabolites were detected using 16S rRNA sequencing and untargeted metabolomics. Compared to the control, no significant differences in α-diversity were observed at weeks 2, 4 and 8. Nevertheless, there were clear differences in community structures at different time points. The phylum Verrucomicrobia significantly declined from 2 to 8 weeks of SMG, yet the relative abundance of Actinobacteria and Deferribacteres expanded remarkably at weeks 8. SMG decreased the genus of Allobaculum and increased Bacteroides significantly throughout the period of 8 weeks. Besides, Genus Akkermansia, Gracilibacter, Prevotella, Odoribacter, Rothia, Sporosarcina, Gracilibacter, Clostridium, and Mucispirillum were identified as biomarkers for SMG group. Desulfovibrio_c21_c20, Akkermansia_muciniphila, and Ruminococcus_gnavus dropped at week 2, which tend to recover at week 4, except for Akkermansia_muciniphila. Bacteroides_uniformis and Faecalibacterium_prausnitzii declined significantly, while Ruminococcus_flavefaciens and Mucispirillum_schaedleri elevated at week 8. Furthermore, intestinal metabolome analysis showed that 129 were upregulated and 146 metabolites were downregulated in SMG. Long-term SMG most affected steroid hormone biosynthesis, tryptophan, cysteine, methionine, arginine, proline metabolism, and histidine metabolism. Correlated analysis suggested that the potential beneficial taxa Allobaculum, Akkermansia, and Faecalibacterium were negatively associated with tryptophan, histidine, arginine, and proline metabolism, but positively with steroid hormone biosynthesis. Yet Bacteroides, Lachnospiraceae_Clostridium, Rothia, Bilophila, and Coprococcus were positively correlated with arginine, proline, tryptophan, and histidine metabolism, while negatively associated with steroid hormone biosynthesis. These results suggest that Long-term SMG altered the community of intestinal microbiota, and then further disturbed intestinal metabolites and metabolic pathways, which have great potential to help understand and provide clues for revealing the mechanisms of long-term SMG involved diseases. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10080065/ /pubmed/37032862 http://dx.doi.org/10.3389/fmicb.2023.1100747 Text en Copyright © 2023 Yuan, Zhang, Li, Gao, Hu, Hussain, Zhang, Wang, Ma, Pan, Lou and Si. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yuan, Lu Zhang, Rong Li, Xinlou Gao, Caiyun Hu, Xiangnan Hussain, Safdar Zhang, Linlin Wang, Moye Ma, Xiaoyu Pan, Qiuxia Lou, Xiaotong Si, Shaoyan Long-term simulated microgravity alters gut microbiota and metabolome in mice |
title | Long-term simulated microgravity alters gut microbiota and metabolome in mice |
title_full | Long-term simulated microgravity alters gut microbiota and metabolome in mice |
title_fullStr | Long-term simulated microgravity alters gut microbiota and metabolome in mice |
title_full_unstemmed | Long-term simulated microgravity alters gut microbiota and metabolome in mice |
title_short | Long-term simulated microgravity alters gut microbiota and metabolome in mice |
title_sort | long-term simulated microgravity alters gut microbiota and metabolome in mice |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080065/ https://www.ncbi.nlm.nih.gov/pubmed/37032862 http://dx.doi.org/10.3389/fmicb.2023.1100747 |
work_keys_str_mv | AT yuanlu longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT zhangrong longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT lixinlou longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT gaocaiyun longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT huxiangnan longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT hussainsafdar longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT zhanglinlin longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT wangmoye longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT maxiaoyu longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT panqiuxia longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT louxiaotong longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice AT sishaoyan longtermsimulatedmicrogravityaltersgutmicrobiotaandmetabolomeinmice |