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Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus

The ongoing outbreak of the monkeypox, caused by monkeypox virus (MPXV), has been a public health emergency of international concern, indicating an urgent need for rapid and sensitive MPXV detection. Here, we designed a diagnostic test based on loop-mediated isothermal amplification (LAMP) and nanop...

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Autores principales: Huang, Xiaolan, Xiao, Fei, Jia, Nan, Sun, Chunrong, Fu, Jin, Xu, Zheng, Cui, Xiaodai, Huang, Hui, Qu, Dong, Zhou, Juan, Wang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080115/
https://www.ncbi.nlm.nih.gov/pubmed/37033067
http://dx.doi.org/10.3389/fpubh.2023.1132896
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author Huang, Xiaolan
Xiao, Fei
Jia, Nan
Sun, Chunrong
Fu, Jin
Xu, Zheng
Cui, Xiaodai
Huang, Hui
Qu, Dong
Zhou, Juan
Wang, Yi
author_facet Huang, Xiaolan
Xiao, Fei
Jia, Nan
Sun, Chunrong
Fu, Jin
Xu, Zheng
Cui, Xiaodai
Huang, Hui
Qu, Dong
Zhou, Juan
Wang, Yi
author_sort Huang, Xiaolan
collection PubMed
description The ongoing outbreak of the monkeypox, caused by monkeypox virus (MPXV), has been a public health emergency of international concern, indicating an urgent need for rapid and sensitive MPXV detection. Here, we designed a diagnostic test based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor(LFB)for diagnosis of MPXV infection, termed MPX-LAMP-LFB. A set of six LAMP primers was designed based the ATI gene of MPXV, and LAMP amplification of MPXV templates was performed at 63°C for only 40 min. The results were rapidly and visually decided using the LFB test within 2 min. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens. The sensitivity of the MPX-LAMP-LFB assay is as low as 5 copies/μl of plasmid template and 12.5 copies/μl of pseudovirus in human blood samples. The whole process of the MPX-LAMP-LFB assay could be completed ~1 h, including rapid template preparation (15 min), LAMP reaction (40 min)and result reporting (<2 min). Collectively, MPX-LAMP-LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection.
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spelling pubmed-100801152023-04-08 Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus Huang, Xiaolan Xiao, Fei Jia, Nan Sun, Chunrong Fu, Jin Xu, Zheng Cui, Xiaodai Huang, Hui Qu, Dong Zhou, Juan Wang, Yi Front Public Health Public Health The ongoing outbreak of the monkeypox, caused by monkeypox virus (MPXV), has been a public health emergency of international concern, indicating an urgent need for rapid and sensitive MPXV detection. Here, we designed a diagnostic test based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor(LFB)for diagnosis of MPXV infection, termed MPX-LAMP-LFB. A set of six LAMP primers was designed based the ATI gene of MPXV, and LAMP amplification of MPXV templates was performed at 63°C for only 40 min. The results were rapidly and visually decided using the LFB test within 2 min. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens. The sensitivity of the MPX-LAMP-LFB assay is as low as 5 copies/μl of plasmid template and 12.5 copies/μl of pseudovirus in human blood samples. The whole process of the MPX-LAMP-LFB assay could be completed ~1 h, including rapid template preparation (15 min), LAMP reaction (40 min)and result reporting (<2 min). Collectively, MPX-LAMP-LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10080115/ /pubmed/37033067 http://dx.doi.org/10.3389/fpubh.2023.1132896 Text en Copyright © 2023 Huang, Xiao, Jia, Sun, Fu, Xu, Cui, Huang, Qu, Zhou and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Huang, Xiaolan
Xiao, Fei
Jia, Nan
Sun, Chunrong
Fu, Jin
Xu, Zheng
Cui, Xiaodai
Huang, Hui
Qu, Dong
Zhou, Juan
Wang, Yi
Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
title Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
title_full Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
title_fullStr Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
title_full_unstemmed Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
title_short Loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
title_sort loop-mediated isothermal amplification combined with lateral flow biosensor for rapid and sensitive detection of monkeypox virus
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080115/
https://www.ncbi.nlm.nih.gov/pubmed/37033067
http://dx.doi.org/10.3389/fpubh.2023.1132896
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