Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure

Introduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that i...

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Autores principales: McCallinhart, Patricia E., Lee, Yong Ung, Lee, Avione, Anghelescu, Mircea, Tonniges, Jeffrey R., Calomeni, Ed, Agarwal, Gunjan, Lincoln, Joy, Trask, Aaron J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080125/
https://www.ncbi.nlm.nih.gov/pubmed/37035668
http://dx.doi.org/10.3389/fphys.2023.1154454
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author McCallinhart, Patricia E.
Lee, Yong Ung
Lee, Avione
Anghelescu, Mircea
Tonniges, Jeffrey R.
Calomeni, Ed
Agarwal, Gunjan
Lincoln, Joy
Trask, Aaron J.
author_facet McCallinhart, Patricia E.
Lee, Yong Ung
Lee, Avione
Anghelescu, Mircea
Tonniges, Jeffrey R.
Calomeni, Ed
Agarwal, Gunjan
Lincoln, Joy
Trask, Aaron J.
author_sort McCallinhart, Patricia E.
collection PubMed
description Introduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that increased aortic PWV in type 2 diabetic mice is driven by increased blood pressure rather than vascular biomechanics. Methods and Results: Using a combination of in vivo PWV and ex vivo pressure myography, our data demonstrate no difference in ex vivo passive mechanics, including outer diameter, inner diameter, compliance (Db/db: 0.0094 ± 0.0018 mm(2)/mmHg vs. db/db: 0.0080 ± 0.0008 mm(2)/mmHg, p > 0.05 at 100 mmHg), and incremental modulus (Db/db: 801.52 ± 135.87 kPa vs. db/db: 838.12 ± 44.90 kPa, p > 0.05 at 100 mmHg), in normal versus diabetic 16 week old mice. We further report no difference in basal or active aorta biomechanics in normal versus diabetic 16 week old mice. Finally, we show here that the increase in diabetic in vivo aortic pulse wave velocity at baseline was completely abolished when measured at equivalent pharmacologically-modulated blood pressures, indicating that the elevated PWV was attributed to the concomitant increase in blood pressure at baseline, and therefore “stiffness.” Conclusions: Together, these animal model data suggest an intimate regulation of blood pressure during collection of pulse wave velocity when determining in vivo vascular stiffness. These data further indicate caution should be exerted when interpreting elevated PWV as the pure marker of vascular stiffness.
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spelling pubmed-100801252023-04-08 Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure McCallinhart, Patricia E. Lee, Yong Ung Lee, Avione Anghelescu, Mircea Tonniges, Jeffrey R. Calomeni, Ed Agarwal, Gunjan Lincoln, Joy Trask, Aaron J. Front Physiol Physiology Introduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that increased aortic PWV in type 2 diabetic mice is driven by increased blood pressure rather than vascular biomechanics. Methods and Results: Using a combination of in vivo PWV and ex vivo pressure myography, our data demonstrate no difference in ex vivo passive mechanics, including outer diameter, inner diameter, compliance (Db/db: 0.0094 ± 0.0018 mm(2)/mmHg vs. db/db: 0.0080 ± 0.0008 mm(2)/mmHg, p > 0.05 at 100 mmHg), and incremental modulus (Db/db: 801.52 ± 135.87 kPa vs. db/db: 838.12 ± 44.90 kPa, p > 0.05 at 100 mmHg), in normal versus diabetic 16 week old mice. We further report no difference in basal or active aorta biomechanics in normal versus diabetic 16 week old mice. Finally, we show here that the increase in diabetic in vivo aortic pulse wave velocity at baseline was completely abolished when measured at equivalent pharmacologically-modulated blood pressures, indicating that the elevated PWV was attributed to the concomitant increase in blood pressure at baseline, and therefore “stiffness.” Conclusions: Together, these animal model data suggest an intimate regulation of blood pressure during collection of pulse wave velocity when determining in vivo vascular stiffness. These data further indicate caution should be exerted when interpreting elevated PWV as the pure marker of vascular stiffness. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10080125/ /pubmed/37035668 http://dx.doi.org/10.3389/fphys.2023.1154454 Text en Copyright © 2023 McCallinhart, Lee, Lee, Anghelescu, Tonniges, Calomeni, Agarwal, Lincoln and Trask. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
McCallinhart, Patricia E.
Lee, Yong Ung
Lee, Avione
Anghelescu, Mircea
Tonniges, Jeffrey R.
Calomeni, Ed
Agarwal, Gunjan
Lincoln, Joy
Trask, Aaron J.
Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
title Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
title_full Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
title_fullStr Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
title_full_unstemmed Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
title_short Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
title_sort dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: the influence of blood pressure
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080125/
https://www.ncbi.nlm.nih.gov/pubmed/37035668
http://dx.doi.org/10.3389/fphys.2023.1154454
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