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Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks

In 2020, a chicken-origin Cluster 3 Tembusu virus (TMUV) caused outbreaks of a disease characterized by egg-drop syndrome in laying hens in China. In the present study, a TMUV strain, TMUV-GX, was isolated from tissue samples of laying hens with egg drop syndrome in south China. Phylogenetic analysi...

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Autores principales: Huang, Yunzhen, Hu, Ziqiang, Dong, Jiawen, Li, Linlin, Zhang, Junqin, Kuang, Ruihuan, Gao, Shimin, Sun, Minhua, Liao, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080150/
https://www.ncbi.nlm.nih.gov/pubmed/37035806
http://dx.doi.org/10.3389/fvets.2023.1152802
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author Huang, Yunzhen
Hu, Ziqiang
Dong, Jiawen
Li, Linlin
Zhang, Junqin
Kuang, Ruihuan
Gao, Shimin
Sun, Minhua
Liao, Ming
author_facet Huang, Yunzhen
Hu, Ziqiang
Dong, Jiawen
Li, Linlin
Zhang, Junqin
Kuang, Ruihuan
Gao, Shimin
Sun, Minhua
Liao, Ming
author_sort Huang, Yunzhen
collection PubMed
description In 2020, a chicken-origin Cluster 3 Tembusu virus (TMUV) caused outbreaks of a disease characterized by egg-drop syndrome in laying hens in China. In the present study, a TMUV strain, TMUV-GX, was isolated from tissue samples of laying hens with egg drop syndrome in south China. Phylogenetic analysis grouped TMUV-GX into TMUV Cluster 3.2, which was distinct from the prevalent TMUV Cluster 2 in duck flocks. To study the infectivity and pathogenicity of TMUV-GX in chickens and ducks, 7 day-old specific pathogen-free (SPF) chicks and SPF ducklings were infected with the same dose of the TMUV-GX. As a comparison, the duck-origin Cluster 2 strain, TMUV-JM, infection groups were set up in chicks and ducklings. Compared with the low infectivity and pathogenicity of TMUV-JM in chicks, the chicken-origin TMUV-GX displayed high replication competence in multiple tissues and caused tissues histopathological damage. In addition, the replication competence of TMUV-GX in ducklings was comparable to that of TMUV-JM. Our study revealed chicken-origin Cluster 3.2 TMUV exhibits high infectivity in chicks and ducklings, and suggested that chicken-origin Cluster 3.2 TMUV possesses a biological basis for widespread infection of chickens and ducks.
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spelling pubmed-100801502023-04-08 Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks Huang, Yunzhen Hu, Ziqiang Dong, Jiawen Li, Linlin Zhang, Junqin Kuang, Ruihuan Gao, Shimin Sun, Minhua Liao, Ming Front Vet Sci Veterinary Science In 2020, a chicken-origin Cluster 3 Tembusu virus (TMUV) caused outbreaks of a disease characterized by egg-drop syndrome in laying hens in China. In the present study, a TMUV strain, TMUV-GX, was isolated from tissue samples of laying hens with egg drop syndrome in south China. Phylogenetic analysis grouped TMUV-GX into TMUV Cluster 3.2, which was distinct from the prevalent TMUV Cluster 2 in duck flocks. To study the infectivity and pathogenicity of TMUV-GX in chickens and ducks, 7 day-old specific pathogen-free (SPF) chicks and SPF ducklings were infected with the same dose of the TMUV-GX. As a comparison, the duck-origin Cluster 2 strain, TMUV-JM, infection groups were set up in chicks and ducklings. Compared with the low infectivity and pathogenicity of TMUV-JM in chicks, the chicken-origin TMUV-GX displayed high replication competence in multiple tissues and caused tissues histopathological damage. In addition, the replication competence of TMUV-GX in ducklings was comparable to that of TMUV-JM. Our study revealed chicken-origin Cluster 3.2 TMUV exhibits high infectivity in chicks and ducklings, and suggested that chicken-origin Cluster 3.2 TMUV possesses a biological basis for widespread infection of chickens and ducks. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10080150/ /pubmed/37035806 http://dx.doi.org/10.3389/fvets.2023.1152802 Text en Copyright © 2023 Huang, Hu, Dong, Li, Zhang, Kuang, Gao, Sun and Liao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Huang, Yunzhen
Hu, Ziqiang
Dong, Jiawen
Li, Linlin
Zhang, Junqin
Kuang, Ruihuan
Gao, Shimin
Sun, Minhua
Liao, Ming
Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks
title Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks
title_full Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks
title_fullStr Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks
title_full_unstemmed Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks
title_short Chicken-origin Cluster 3.2 Tembusu virus exhibits higher infectivity than duck-origin Cluster 2 Tembusu virus in chicks
title_sort chicken-origin cluster 3.2 tembusu virus exhibits higher infectivity than duck-origin cluster 2 tembusu virus in chicks
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080150/
https://www.ncbi.nlm.nih.gov/pubmed/37035806
http://dx.doi.org/10.3389/fvets.2023.1152802
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