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Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway

Acute pancreatitis is an inflammatory response in the pancreas, involving activation of pancreatic enzymes. Severe acute pancreatitis (SAP) often causes systemic complications that affect distant organs, including the lungs. The aim of this study was to explore the therapeutic potential of piperlong...

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Autores principales: Hu, Qian, Tao, Ran, Hu, Xiaoyun, Wu, Haibo, Xu, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080291/
https://www.ncbi.nlm.nih.gov/pubmed/36951266
http://dx.doi.org/10.4081/ejh.2023.3639
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author Hu, Qian
Tao, Ran
Hu, Xiaoyun
Wu, Haibo
Xu, Jianjun
author_facet Hu, Qian
Tao, Ran
Hu, Xiaoyun
Wu, Haibo
Xu, Jianjun
author_sort Hu, Qian
collection PubMed
description Acute pancreatitis is an inflammatory response in the pancreas, involving activation of pancreatic enzymes. Severe acute pancreatitis (SAP) often causes systemic complications that affect distant organs, including the lungs. The aim of this study was to explore the therapeutic potential of piperlonguminine on SAP-induced lung injury in rat models. Acute pancreatitis was induced in rats by repetitive injections with 4% sodium taurocholate. Histological examination and biochemical assays were used to assess the severity of lung injury, including tissue damage, and levels of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4), reactive oxygen species (ROS), and inflammatory cytokines. We found that piperlonguminine significantly ameliorated pulmonary architectural distortion, hemorrhage, interstitial edema, and alveolar thickening in rats with SAP. In addition, NOX2, NOX4, ROS, and inflammatory cytokine levels in pulmonary tissues were notably decreased in piperlonguminine- treated rats. Piperlonguminine also attenuated the expression levels of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB). Together, our findings demonstrate for the first time that piperlonguminine can ameliorate acute pancreatitis-induced lung injury via inhibitory modulation of inflammatory responses by suppression of the TLR4/NF-κB signaling pathway.
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spelling pubmed-100802912023-04-08 Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway Hu, Qian Tao, Ran Hu, Xiaoyun Wu, Haibo Xu, Jianjun Eur J Histochem Article Acute pancreatitis is an inflammatory response in the pancreas, involving activation of pancreatic enzymes. Severe acute pancreatitis (SAP) often causes systemic complications that affect distant organs, including the lungs. The aim of this study was to explore the therapeutic potential of piperlonguminine on SAP-induced lung injury in rat models. Acute pancreatitis was induced in rats by repetitive injections with 4% sodium taurocholate. Histological examination and biochemical assays were used to assess the severity of lung injury, including tissue damage, and levels of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4), reactive oxygen species (ROS), and inflammatory cytokines. We found that piperlonguminine significantly ameliorated pulmonary architectural distortion, hemorrhage, interstitial edema, and alveolar thickening in rats with SAP. In addition, NOX2, NOX4, ROS, and inflammatory cytokine levels in pulmonary tissues were notably decreased in piperlonguminine- treated rats. Piperlonguminine also attenuated the expression levels of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB). Together, our findings demonstrate for the first time that piperlonguminine can ameliorate acute pancreatitis-induced lung injury via inhibitory modulation of inflammatory responses by suppression of the TLR4/NF-κB signaling pathway. PAGEPress Publications, Pavia, Italy 2023-03-20 /pmc/articles/PMC10080291/ /pubmed/36951266 http://dx.doi.org/10.4081/ejh.2023.3639 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Hu, Qian
Tao, Ran
Hu, Xiaoyun
Wu, Haibo
Xu, Jianjun
Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway
title Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway
title_full Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway
title_fullStr Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway
title_full_unstemmed Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway
title_short Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway
title_sort effects of piperlonguminine on lung injury in severe acute pancreatitis via the tlr4/nf-κb pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080291/
https://www.ncbi.nlm.nih.gov/pubmed/36951266
http://dx.doi.org/10.4081/ejh.2023.3639
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