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An optimized circular polymerase extension reaction-based method for functional analysis of SARS-CoV-2

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BACKGROUND: Reverse genetics systems have been crucial for studying specific viral genes and their relevance in the virus lifecycle, and become important tools for the rational attenuation of viruses and thereby for vaccine design. Recent rapid progress has been made in the establishment of reverse...

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Detalles Bibliográficos
Autores principales:	Liu, GuanQun, Gack, Michaela U.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080526/ https://www.ncbi.nlm.nih.gov/pubmed/37029393 http://dx.doi.org/10.1186/s12985-023-02025-y

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