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Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis

[Image: see text] The structural complexity of glycans makes their characterization challenging, not only because of the presence of various isomeric forms of the precursor molecule but also because the fragments can themselves be isomeric. We have recently developed an IMS-CID-IMS approach using st...

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Autores principales: Bansal, Priyanka, Ben Faleh, Ahmed, Warnke, Stephan, Rizzo, Thomas R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080682/
https://www.ncbi.nlm.nih.gov/pubmed/36881006
http://dx.doi.org/10.1021/jasms.2c00361
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author Bansal, Priyanka
Ben Faleh, Ahmed
Warnke, Stephan
Rizzo, Thomas R.
author_facet Bansal, Priyanka
Ben Faleh, Ahmed
Warnke, Stephan
Rizzo, Thomas R.
author_sort Bansal, Priyanka
collection PubMed
description [Image: see text] The structural complexity of glycans makes their characterization challenging, not only because of the presence of various isomeric forms of the precursor molecule but also because the fragments can themselves be isomeric. We have recently developed an IMS-CID-IMS approach using structures for lossless ion manipulations (SLIM) combined with cryogenic infrared (IR) spectroscopy for glycan analysis. It allows mobility separation and collision-induced dissociation of a precursor glycan followed by mobility separation and IR spectroscopy of the fragments. While this approach holds great promise for glycan analysis, we often encounter fragments for which we have no standards to identify their spectroscopic fingerprint. In this work, we perform proof-of-principle experiments employing a multistage SLIM-based IMS-CID technique to generate second-generation fragments, followed by their mobility separation and spectroscopic interrogation. This approach provides detailed structural information about the first-generation fragments, including their anomeric form, which in turn can be used to identify the precursor glycan.
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spelling pubmed-100806822023-04-08 Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis Bansal, Priyanka Ben Faleh, Ahmed Warnke, Stephan Rizzo, Thomas R. J Am Soc Mass Spectrom [Image: see text] The structural complexity of glycans makes their characterization challenging, not only because of the presence of various isomeric forms of the precursor molecule but also because the fragments can themselves be isomeric. We have recently developed an IMS-CID-IMS approach using structures for lossless ion manipulations (SLIM) combined with cryogenic infrared (IR) spectroscopy for glycan analysis. It allows mobility separation and collision-induced dissociation of a precursor glycan followed by mobility separation and IR spectroscopy of the fragments. While this approach holds great promise for glycan analysis, we often encounter fragments for which we have no standards to identify their spectroscopic fingerprint. In this work, we perform proof-of-principle experiments employing a multistage SLIM-based IMS-CID technique to generate second-generation fragments, followed by their mobility separation and spectroscopic interrogation. This approach provides detailed structural information about the first-generation fragments, including their anomeric form, which in turn can be used to identify the precursor glycan. American Chemical Society 2023-03-07 /pmc/articles/PMC10080682/ /pubmed/36881006 http://dx.doi.org/10.1021/jasms.2c00361 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Bansal, Priyanka
Ben Faleh, Ahmed
Warnke, Stephan
Rizzo, Thomas R.
Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
title Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
title_full Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
title_fullStr Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
title_full_unstemmed Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
title_short Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
title_sort multistage ion mobility spectrometry combined with infrared spectroscopy for glycan analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080682/
https://www.ncbi.nlm.nih.gov/pubmed/36881006
http://dx.doi.org/10.1021/jasms.2c00361
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