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Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance

Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pra...

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Autores principales: Westerdahl, Becky, Riddle, Lee, Giraud, Deborah, Kamo, Kathryn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081024/
https://www.ncbi.nlm.nih.gov/pubmed/37035051
http://dx.doi.org/10.3389/fpls.2023.1134224
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author Westerdahl, Becky
Riddle, Lee
Giraud, Deborah
Kamo, Kathryn
author_facet Westerdahl, Becky
Riddle, Lee
Giraud, Deborah
Kamo, Kathryn
author_sort Westerdahl, Becky
collection PubMed
description Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pratylenchus penetrans, the root lesion nematode. Easter lilies are one of the most expensive crops to produce because of the cost of chemicals used to control P. penetrans and other pathogens that infect the lilies. Our previous study had shown that transgenic Easter lilies containing a rice cystatin gene (Oc-IΔD86 that has a deleted Asp86) were resistant to P. penetrans in vitro. This study examined growth characteristics of five independently transformed lines of the cystatin Easter lilies compared to non-transformed Nellie White for three seasons in the field in Brookings, Oregon. Liles grown in three soil chemical treatments 1) preplant fumigation, 2) preplant fumigation plus at plant organophosphate, and 3) at plant organophosphate were compared to those grown in nontreated soil. Growth characteristics evaluated included: time of shoot emergence, survival of plants, size of plants, visual ratings of plant health, basal roots and stem roots, weight of foliage and roots, and number and size of bulblets that developed on stems. Nematodes were counted following their extraction from the roots. While not totally resistant, when planted in the field, transformed lines demonstrated and maintained a degree of resistance to lesion nematode over two growing seasons and displayed desirable growth and quality characteristics similar to non-transformed lilies.
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spelling pubmed-100810242023-04-08 Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance Westerdahl, Becky Riddle, Lee Giraud, Deborah Kamo, Kathryn Front Plant Sci Plant Science Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pratylenchus penetrans, the root lesion nematode. Easter lilies are one of the most expensive crops to produce because of the cost of chemicals used to control P. penetrans and other pathogens that infect the lilies. Our previous study had shown that transgenic Easter lilies containing a rice cystatin gene (Oc-IΔD86 that has a deleted Asp86) were resistant to P. penetrans in vitro. This study examined growth characteristics of five independently transformed lines of the cystatin Easter lilies compared to non-transformed Nellie White for three seasons in the field in Brookings, Oregon. Liles grown in three soil chemical treatments 1) preplant fumigation, 2) preplant fumigation plus at plant organophosphate, and 3) at plant organophosphate were compared to those grown in nontreated soil. Growth characteristics evaluated included: time of shoot emergence, survival of plants, size of plants, visual ratings of plant health, basal roots and stem roots, weight of foliage and roots, and number and size of bulblets that developed on stems. Nematodes were counted following their extraction from the roots. While not totally resistant, when planted in the field, transformed lines demonstrated and maintained a degree of resistance to lesion nematode over two growing seasons and displayed desirable growth and quality characteristics similar to non-transformed lilies. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10081024/ /pubmed/37035051 http://dx.doi.org/10.3389/fpls.2023.1134224 Text en Copyright © 2023 Westerdahl, Riddle, Giraud and Kamo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Westerdahl, Becky
Riddle, Lee
Giraud, Deborah
Kamo, Kathryn
Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
title Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
title_full Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
title_fullStr Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
title_full_unstemmed Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
title_short Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
title_sort field test of easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081024/
https://www.ncbi.nlm.nih.gov/pubmed/37035051
http://dx.doi.org/10.3389/fpls.2023.1134224
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