Cargando…
Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance
Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pra...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081024/ https://www.ncbi.nlm.nih.gov/pubmed/37035051 http://dx.doi.org/10.3389/fpls.2023.1134224 |
_version_ | 1785021034923032576 |
---|---|
author | Westerdahl, Becky Riddle, Lee Giraud, Deborah Kamo, Kathryn |
author_facet | Westerdahl, Becky Riddle, Lee Giraud, Deborah Kamo, Kathryn |
author_sort | Westerdahl, Becky |
collection | PubMed |
description | Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pratylenchus penetrans, the root lesion nematode. Easter lilies are one of the most expensive crops to produce because of the cost of chemicals used to control P. penetrans and other pathogens that infect the lilies. Our previous study had shown that transgenic Easter lilies containing a rice cystatin gene (Oc-IΔD86 that has a deleted Asp86) were resistant to P. penetrans in vitro. This study examined growth characteristics of five independently transformed lines of the cystatin Easter lilies compared to non-transformed Nellie White for three seasons in the field in Brookings, Oregon. Liles grown in three soil chemical treatments 1) preplant fumigation, 2) preplant fumigation plus at plant organophosphate, and 3) at plant organophosphate were compared to those grown in nontreated soil. Growth characteristics evaluated included: time of shoot emergence, survival of plants, size of plants, visual ratings of plant health, basal roots and stem roots, weight of foliage and roots, and number and size of bulblets that developed on stems. Nematodes were counted following their extraction from the roots. While not totally resistant, when planted in the field, transformed lines demonstrated and maintained a degree of resistance to lesion nematode over two growing seasons and displayed desirable growth and quality characteristics similar to non-transformed lilies. |
format | Online Article Text |
id | pubmed-10081024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100810242023-04-08 Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance Westerdahl, Becky Riddle, Lee Giraud, Deborah Kamo, Kathryn Front Plant Sci Plant Science Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pratylenchus penetrans, the root lesion nematode. Easter lilies are one of the most expensive crops to produce because of the cost of chemicals used to control P. penetrans and other pathogens that infect the lilies. Our previous study had shown that transgenic Easter lilies containing a rice cystatin gene (Oc-IΔD86 that has a deleted Asp86) were resistant to P. penetrans in vitro. This study examined growth characteristics of five independently transformed lines of the cystatin Easter lilies compared to non-transformed Nellie White for three seasons in the field in Brookings, Oregon. Liles grown in three soil chemical treatments 1) preplant fumigation, 2) preplant fumigation plus at plant organophosphate, and 3) at plant organophosphate were compared to those grown in nontreated soil. Growth characteristics evaluated included: time of shoot emergence, survival of plants, size of plants, visual ratings of plant health, basal roots and stem roots, weight of foliage and roots, and number and size of bulblets that developed on stems. Nematodes were counted following their extraction from the roots. While not totally resistant, when planted in the field, transformed lines demonstrated and maintained a degree of resistance to lesion nematode over two growing seasons and displayed desirable growth and quality characteristics similar to non-transformed lilies. Frontiers Media S.A. 2023-03-24 /pmc/articles/PMC10081024/ /pubmed/37035051 http://dx.doi.org/10.3389/fpls.2023.1134224 Text en Copyright © 2023 Westerdahl, Riddle, Giraud and Kamo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Westerdahl, Becky Riddle, Lee Giraud, Deborah Kamo, Kathryn Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
title | Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
title_full | Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
title_fullStr | Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
title_full_unstemmed | Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
title_short | Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
title_sort | field test of easter lilies transformed with a rice cystatin gene for root lesion nematode resistance |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081024/ https://www.ncbi.nlm.nih.gov/pubmed/37035051 http://dx.doi.org/10.3389/fpls.2023.1134224 |
work_keys_str_mv | AT westerdahlbecky fieldtestofeasterliliestransformedwitharicecystatingeneforrootlesionnematoderesistance AT riddlelee fieldtestofeasterliliestransformedwitharicecystatingeneforrootlesionnematoderesistance AT girauddeborah fieldtestofeasterliliestransformedwitharicecystatingeneforrootlesionnematoderesistance AT kamokathryn fieldtestofeasterliliestransformedwitharicecystatingeneforrootlesionnematoderesistance |