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Determination of Embryotoxic Effects of In Ovo Administered Propofol on Peripheral Blood Alpha Naphthyl Acetate Esterase and Acid Phosphatase-Positive Lymphocytes
OBJECTIVE: The aim of this study is to determine the possible embryotoxic effects of propofol, a general anaesthetic agent that is commonly used in clinical practice, on peripheral blood lymphocytes using enzyme histochemical techniques. METHODS: For this purpose, 430 laying hen fertile eggs were us...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Turkish Society of Anaesthesiology and Reanimation
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081133/ https://www.ncbi.nlm.nih.gov/pubmed/36847317 http://dx.doi.org/10.5152/TJAR.2023.22726 |
Sumario: | OBJECTIVE: The aim of this study is to determine the possible embryotoxic effects of propofol, a general anaesthetic agent that is commonly used in clinical practice, on peripheral blood lymphocytes using enzyme histochemical techniques. METHODS: For this purpose, 430 laying hen fertile eggs were used for this study. The eggs were divided into 5 groups as control, solvent-control (saline), 2.5 mg kg(−1) propofol, 12.5 mg kg(−1) propofol, and 37.5 mg kg(−1) propofol, and injections were performed via the air sac just before the incubation. The peripheral blood alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios were determined on the hatching day. RESULTS: No statistically significant difference was found between both alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios of the control and solvent-control groups. However, when compared with the control and solvent-control groups, statistically significant decreases were observed in the peripheral blood alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios of the chicks from the propofol-injected groups. Besides, the difference between 2.5 mg kg(−1) and 12.5 mg kg(−1) propofol groups is not significant, whereas the difference between these 2 groups and the 37.5 mg kg(−1) propofol group was statistically significant (P < .05). CONCLUSIONS: It was concluded that propofol given to fertilised chicken eggs just before incubation caused significant decreases in both the peripheral blood alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios. |
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