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Matrix vesicles promote bone repair after a femoral bone defect in mice

Matrix vesicles (MtVs) are one of the extracellular vesicles (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator of ossification and recent findings suggest a role for MtVs in the regulation of bone cell biology, the effects of MtVs on bone repair remain...

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Autores principales: Mizukami, Yuya, Kawao, Naoyuki, Takafuji, Yoshimasa, Ohira, Takashi, Okada, Kiyotaka, Jo, Jun-Ichiro, Tabata, Yasuhiko, Kaji, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081784/
https://www.ncbi.nlm.nih.gov/pubmed/37027385
http://dx.doi.org/10.1371/journal.pone.0284258
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author Mizukami, Yuya
Kawao, Naoyuki
Takafuji, Yoshimasa
Ohira, Takashi
Okada, Kiyotaka
Jo, Jun-Ichiro
Tabata, Yasuhiko
Kaji, Hiroshi
author_facet Mizukami, Yuya
Kawao, Naoyuki
Takafuji, Yoshimasa
Ohira, Takashi
Okada, Kiyotaka
Jo, Jun-Ichiro
Tabata, Yasuhiko
Kaji, Hiroshi
author_sort Mizukami, Yuya
collection PubMed
description Matrix vesicles (MtVs) are one of the extracellular vesicles (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator of ossification and recent findings suggest a role for MtVs in the regulation of bone cell biology, the effects of MtVs on bone repair remain unclear. In the present study, we employed collagenase-released EVs (CREVs) containing abundant MtVs from mouse osteoblasts. CREVs were administered locally in gelatin hydrogels to damaged sites after a femoral bone defect in mice. CREVs exhibited the characteristics of MtVs with a diameter <200 nm. The local administration of CREVs significantly promoted the formation of new bone with increases in the number of alkaline phosphatase (ALP)-positive cells and cartilage formation at the damaged site after the femoral bone defect. However, the addition of CREVs to the medium did not promote the osteogenic differentiation of ST2 cells or the ALP activity or mineralization of mouse osteoblasts in vitro. In conclusion, we herein showed for the first time that MtVs enhanced bone repair after a femoral bone defect partly through osteogenesis and chondrogenesis in mice. Therefore, MtVs have potential as a tool for bone regeneration.
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spelling pubmed-100817842023-04-08 Matrix vesicles promote bone repair after a femoral bone defect in mice Mizukami, Yuya Kawao, Naoyuki Takafuji, Yoshimasa Ohira, Takashi Okada, Kiyotaka Jo, Jun-Ichiro Tabata, Yasuhiko Kaji, Hiroshi PLoS One Research Article Matrix vesicles (MtVs) are one of the extracellular vesicles (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator of ossification and recent findings suggest a role for MtVs in the regulation of bone cell biology, the effects of MtVs on bone repair remain unclear. In the present study, we employed collagenase-released EVs (CREVs) containing abundant MtVs from mouse osteoblasts. CREVs were administered locally in gelatin hydrogels to damaged sites after a femoral bone defect in mice. CREVs exhibited the characteristics of MtVs with a diameter <200 nm. The local administration of CREVs significantly promoted the formation of new bone with increases in the number of alkaline phosphatase (ALP)-positive cells and cartilage formation at the damaged site after the femoral bone defect. However, the addition of CREVs to the medium did not promote the osteogenic differentiation of ST2 cells or the ALP activity or mineralization of mouse osteoblasts in vitro. In conclusion, we herein showed for the first time that MtVs enhanced bone repair after a femoral bone defect partly through osteogenesis and chondrogenesis in mice. Therefore, MtVs have potential as a tool for bone regeneration. Public Library of Science 2023-04-07 /pmc/articles/PMC10081784/ /pubmed/37027385 http://dx.doi.org/10.1371/journal.pone.0284258 Text en © 2023 Mizukami et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mizukami, Yuya
Kawao, Naoyuki
Takafuji, Yoshimasa
Ohira, Takashi
Okada, Kiyotaka
Jo, Jun-Ichiro
Tabata, Yasuhiko
Kaji, Hiroshi
Matrix vesicles promote bone repair after a femoral bone defect in mice
title Matrix vesicles promote bone repair after a femoral bone defect in mice
title_full Matrix vesicles promote bone repair after a femoral bone defect in mice
title_fullStr Matrix vesicles promote bone repair after a femoral bone defect in mice
title_full_unstemmed Matrix vesicles promote bone repair after a femoral bone defect in mice
title_short Matrix vesicles promote bone repair after a femoral bone defect in mice
title_sort matrix vesicles promote bone repair after a femoral bone defect in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081784/
https://www.ncbi.nlm.nih.gov/pubmed/37027385
http://dx.doi.org/10.1371/journal.pone.0284258
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