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Matrix vesicles promote bone repair after a femoral bone defect in mice
Matrix vesicles (MtVs) are one of the extracellular vesicles (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator of ossification and recent findings suggest a role for MtVs in the regulation of bone cell biology, the effects of MtVs on bone repair remain...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081784/ https://www.ncbi.nlm.nih.gov/pubmed/37027385 http://dx.doi.org/10.1371/journal.pone.0284258 |
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author | Mizukami, Yuya Kawao, Naoyuki Takafuji, Yoshimasa Ohira, Takashi Okada, Kiyotaka Jo, Jun-Ichiro Tabata, Yasuhiko Kaji, Hiroshi |
author_facet | Mizukami, Yuya Kawao, Naoyuki Takafuji, Yoshimasa Ohira, Takashi Okada, Kiyotaka Jo, Jun-Ichiro Tabata, Yasuhiko Kaji, Hiroshi |
author_sort | Mizukami, Yuya |
collection | PubMed |
description | Matrix vesicles (MtVs) are one of the extracellular vesicles (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator of ossification and recent findings suggest a role for MtVs in the regulation of bone cell biology, the effects of MtVs on bone repair remain unclear. In the present study, we employed collagenase-released EVs (CREVs) containing abundant MtVs from mouse osteoblasts. CREVs were administered locally in gelatin hydrogels to damaged sites after a femoral bone defect in mice. CREVs exhibited the characteristics of MtVs with a diameter <200 nm. The local administration of CREVs significantly promoted the formation of new bone with increases in the number of alkaline phosphatase (ALP)-positive cells and cartilage formation at the damaged site after the femoral bone defect. However, the addition of CREVs to the medium did not promote the osteogenic differentiation of ST2 cells or the ALP activity or mineralization of mouse osteoblasts in vitro. In conclusion, we herein showed for the first time that MtVs enhanced bone repair after a femoral bone defect partly through osteogenesis and chondrogenesis in mice. Therefore, MtVs have potential as a tool for bone regeneration. |
format | Online Article Text |
id | pubmed-10081784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100817842023-04-08 Matrix vesicles promote bone repair after a femoral bone defect in mice Mizukami, Yuya Kawao, Naoyuki Takafuji, Yoshimasa Ohira, Takashi Okada, Kiyotaka Jo, Jun-Ichiro Tabata, Yasuhiko Kaji, Hiroshi PLoS One Research Article Matrix vesicles (MtVs) are one of the extracellular vesicles (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator of ossification and recent findings suggest a role for MtVs in the regulation of bone cell biology, the effects of MtVs on bone repair remain unclear. In the present study, we employed collagenase-released EVs (CREVs) containing abundant MtVs from mouse osteoblasts. CREVs were administered locally in gelatin hydrogels to damaged sites after a femoral bone defect in mice. CREVs exhibited the characteristics of MtVs with a diameter <200 nm. The local administration of CREVs significantly promoted the formation of new bone with increases in the number of alkaline phosphatase (ALP)-positive cells and cartilage formation at the damaged site after the femoral bone defect. However, the addition of CREVs to the medium did not promote the osteogenic differentiation of ST2 cells or the ALP activity or mineralization of mouse osteoblasts in vitro. In conclusion, we herein showed for the first time that MtVs enhanced bone repair after a femoral bone defect partly through osteogenesis and chondrogenesis in mice. Therefore, MtVs have potential as a tool for bone regeneration. Public Library of Science 2023-04-07 /pmc/articles/PMC10081784/ /pubmed/37027385 http://dx.doi.org/10.1371/journal.pone.0284258 Text en © 2023 Mizukami et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mizukami, Yuya Kawao, Naoyuki Takafuji, Yoshimasa Ohira, Takashi Okada, Kiyotaka Jo, Jun-Ichiro Tabata, Yasuhiko Kaji, Hiroshi Matrix vesicles promote bone repair after a femoral bone defect in mice |
title | Matrix vesicles promote bone repair after a femoral bone defect in mice |
title_full | Matrix vesicles promote bone repair after a femoral bone defect in mice |
title_fullStr | Matrix vesicles promote bone repair after a femoral bone defect in mice |
title_full_unstemmed | Matrix vesicles promote bone repair after a femoral bone defect in mice |
title_short | Matrix vesicles promote bone repair after a femoral bone defect in mice |
title_sort | matrix vesicles promote bone repair after a femoral bone defect in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081784/ https://www.ncbi.nlm.nih.gov/pubmed/37027385 http://dx.doi.org/10.1371/journal.pone.0284258 |
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