Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells

This study provides a combined histochemical method for detecting enzyme activity of chloroacetate esterase simultaneously with immunolabeling of the components of a specific tissue microenvironment on formalin-fixed, paraffin-embedded specimens. Chromogenic detection of the molecular targets within...

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Autores principales: Atiakshin, Dmitri, Kostin, Andrey, Buchwalow, Igor, Morozow, Denis, Samoilova, Vera, Tiemann, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081974/
https://www.ncbi.nlm.nih.gov/pubmed/36598563
http://dx.doi.org/10.1007/s00418-022-02174-1
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author Atiakshin, Dmitri
Kostin, Andrey
Buchwalow, Igor
Morozow, Denis
Samoilova, Vera
Tiemann, Markus
author_facet Atiakshin, Dmitri
Kostin, Andrey
Buchwalow, Igor
Morozow, Denis
Samoilova, Vera
Tiemann, Markus
author_sort Atiakshin, Dmitri
collection PubMed
description This study provides a combined histochemical method for detecting enzyme activity of chloroacetate esterase simultaneously with immunolabeling of the components of a specific tissue microenvironment on formalin-fixed, paraffin-embedded specimens. Chromogenic detection of the molecular targets within and outside the mast cells provides novel options in determining the histoarchitectonics of organ-specific mast cell populations, studying the functional significance of chloroacetate esterase and specifying the immune landscape of the tissue microenvironment.
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spelling pubmed-100819742023-04-09 Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells Atiakshin, Dmitri Kostin, Andrey Buchwalow, Igor Morozow, Denis Samoilova, Vera Tiemann, Markus Histochem Cell Biol Original Paper This study provides a combined histochemical method for detecting enzyme activity of chloroacetate esterase simultaneously with immunolabeling of the components of a specific tissue microenvironment on formalin-fixed, paraffin-embedded specimens. Chromogenic detection of the molecular targets within and outside the mast cells provides novel options in determining the histoarchitectonics of organ-specific mast cell populations, studying the functional significance of chloroacetate esterase and specifying the immune landscape of the tissue microenvironment. Springer Berlin Heidelberg 2023-01-04 2023 /pmc/articles/PMC10081974/ /pubmed/36598563 http://dx.doi.org/10.1007/s00418-022-02174-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Atiakshin, Dmitri
Kostin, Andrey
Buchwalow, Igor
Morozow, Denis
Samoilova, Vera
Tiemann, Markus
Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
title Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
title_full Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
title_fullStr Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
title_full_unstemmed Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
title_short Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
title_sort chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081974/
https://www.ncbi.nlm.nih.gov/pubmed/36598563
http://dx.doi.org/10.1007/s00418-022-02174-1
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