Cargando…

First continuous marine sponge cell line established

The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted to culture marine sponge cells in vitro to create a scalable production platform for such biopharmaceuticals, these efforts have been m...

Descripción completa

Detalles Bibliográficos
Autores principales: Hesp, Kylie, van der Heijden, Jans M. E., Munroe, Stephanie, Sipkema, Detmer, Martens, Dirk E., Wijffels, Rene H., Pomponi, Shirley A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10082835/
https://www.ncbi.nlm.nih.gov/pubmed/37031251
http://dx.doi.org/10.1038/s41598-023-32394-x
_version_ 1785021396705869824
author Hesp, Kylie
van der Heijden, Jans M. E.
Munroe, Stephanie
Sipkema, Detmer
Martens, Dirk E.
Wijffels, Rene H.
Pomponi, Shirley A.
author_facet Hesp, Kylie
van der Heijden, Jans M. E.
Munroe, Stephanie
Sipkema, Detmer
Martens, Dirk E.
Wijffels, Rene H.
Pomponi, Shirley A.
author_sort Hesp, Kylie
collection PubMed
description The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted to culture marine sponge cells in vitro to create a scalable production platform for such biopharmaceuticals, these efforts have been mostly unsuccessful. We recently showed that Geodia barretti sponge cells could divide rapidly in M1 medium. In this study we established the first continuous marine sponge cell line, originating from G. barretti. G. barretti cells cultured in OpM1 medium, a modification of M1, grew more rapidly and to a higher density than in M1. Cells in OpM1 reached 1.74 population doublings after 30 min, more than twofold higher than the already rapid growth rate of 0.74 population doublings in 30 min in M1. The maximum number of population doublings increased from 5 doublings in M1 to at least 98 doublings in OpM1. Subcultured cells could be cryopreserved and used to inoculate new cultures. With these results, we have overcome a major obstacle that has blocked the path to producing biopharmaceuticals with sponge cells at industrial scale for decades.
format Online
Article
Text
id pubmed-10082835
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-100828352023-04-10 First continuous marine sponge cell line established Hesp, Kylie van der Heijden, Jans M. E. Munroe, Stephanie Sipkema, Detmer Martens, Dirk E. Wijffels, Rene H. Pomponi, Shirley A. Sci Rep Article The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted to culture marine sponge cells in vitro to create a scalable production platform for such biopharmaceuticals, these efforts have been mostly unsuccessful. We recently showed that Geodia barretti sponge cells could divide rapidly in M1 medium. In this study we established the first continuous marine sponge cell line, originating from G. barretti. G. barretti cells cultured in OpM1 medium, a modification of M1, grew more rapidly and to a higher density than in M1. Cells in OpM1 reached 1.74 population doublings after 30 min, more than twofold higher than the already rapid growth rate of 0.74 population doublings in 30 min in M1. The maximum number of population doublings increased from 5 doublings in M1 to at least 98 doublings in OpM1. Subcultured cells could be cryopreserved and used to inoculate new cultures. With these results, we have overcome a major obstacle that has blocked the path to producing biopharmaceuticals with sponge cells at industrial scale for decades. Nature Publishing Group UK 2023-04-08 /pmc/articles/PMC10082835/ /pubmed/37031251 http://dx.doi.org/10.1038/s41598-023-32394-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hesp, Kylie
van der Heijden, Jans M. E.
Munroe, Stephanie
Sipkema, Detmer
Martens, Dirk E.
Wijffels, Rene H.
Pomponi, Shirley A.
First continuous marine sponge cell line established
title First continuous marine sponge cell line established
title_full First continuous marine sponge cell line established
title_fullStr First continuous marine sponge cell line established
title_full_unstemmed First continuous marine sponge cell line established
title_short First continuous marine sponge cell line established
title_sort first continuous marine sponge cell line established
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10082835/
https://www.ncbi.nlm.nih.gov/pubmed/37031251
http://dx.doi.org/10.1038/s41598-023-32394-x
work_keys_str_mv AT hespkylie firstcontinuousmarinespongecelllineestablished
AT vanderheijdenjansme firstcontinuousmarinespongecelllineestablished
AT munroestephanie firstcontinuousmarinespongecelllineestablished
AT sipkemadetmer firstcontinuousmarinespongecelllineestablished
AT martensdirke firstcontinuousmarinespongecelllineestablished
AT wijffelsreneh firstcontinuousmarinespongecelllineestablished
AT pomponishirleya firstcontinuousmarinespongecelllineestablished