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Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy
BACKGROUND: Idiopathic membranous nephropathy (IMN) is an organ-specific autoimmune disease with multiple and complex pathogenic mechanisms. Currently, renal biopsy is considered the gold standard for diagnosing membranous nephropathy. However, there were limitations to the renal puncture biopsy, su...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083006/ https://www.ncbi.nlm.nih.gov/pubmed/37041975 http://dx.doi.org/10.7717/peerj.15167 |
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author | Ye, Mingjun Tang, Donge Li, Weilong Ma, Chiyu Zeng, Zhipeng Liao, Shengyou Song, Zhuoheng Meng, Yu Liu, Fanna Luan, Shaodong Yin, Lianghong Dai, Yong |
author_facet | Ye, Mingjun Tang, Donge Li, Weilong Ma, Chiyu Zeng, Zhipeng Liao, Shengyou Song, Zhuoheng Meng, Yu Liu, Fanna Luan, Shaodong Yin, Lianghong Dai, Yong |
author_sort | Ye, Mingjun |
collection | PubMed |
description | BACKGROUND: Idiopathic membranous nephropathy (IMN) is an organ-specific autoimmune disease with multiple and complex pathogenic mechanisms. Currently, renal biopsy is considered the gold standard for diagnosing membranous nephropathy. However, there were limitations to the renal puncture biopsy, such as the relatively high cost, longer time consuming, and the risk of invasive procedures. We investigated the profile of serum metabolites in IMN patients based on the UHPLC-QE-MS metabolomics technique for exploring the potential disease biomarkers and clinical implementation. METHODS: In our research, we collected serum samples from healthy control (n = 15) and IMN patients (n = 25) to perform metabolomics analysis based on the UHPLC-QE-MS technique. RESULT: We identified 215 differentially expressed metabolites (DEMs) between the IMN and healthy control (HC) groups. Furthermore, these DEMs were significantly identified in histidine metabolism, arginine and proline metabolism, pyrimidine metabolism, purine metabolism, and steroid hormone biosynthesis. Several key DEMs were significantly correlated with the level of clinical parameters, such as serum albumin, IgG, UTP, and cholesterol. Among them, dehydroepiandrosterone sulfate (DHEAS) was considered the reliable diagnostic biomarker in the IMN group. There was an increased abundance of actinobacteria, phylum proteobacteria, and class gammaproteobacterial in IMN patients for host-microbiome origin analysis. CONCLUSION: Our study revealed the profiles of DEMs from the IMN and HC groups. The result demonstrated that there were disorders of amino acids, nucleotides, and steroids hormones metabolism in IMN patients. The down-regulation of DHEAS may be associated with the imbalance of the immune environment in IMN patients. In host-microbiome origin analysis, the gut microbiota and metabolite disturbances were present in IMN patients. |
format | Online Article Text |
id | pubmed-10083006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100830062023-04-10 Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy Ye, Mingjun Tang, Donge Li, Weilong Ma, Chiyu Zeng, Zhipeng Liao, Shengyou Song, Zhuoheng Meng, Yu Liu, Fanna Luan, Shaodong Yin, Lianghong Dai, Yong PeerJ Bioinformatics BACKGROUND: Idiopathic membranous nephropathy (IMN) is an organ-specific autoimmune disease with multiple and complex pathogenic mechanisms. Currently, renal biopsy is considered the gold standard for diagnosing membranous nephropathy. However, there were limitations to the renal puncture biopsy, such as the relatively high cost, longer time consuming, and the risk of invasive procedures. We investigated the profile of serum metabolites in IMN patients based on the UHPLC-QE-MS metabolomics technique for exploring the potential disease biomarkers and clinical implementation. METHODS: In our research, we collected serum samples from healthy control (n = 15) and IMN patients (n = 25) to perform metabolomics analysis based on the UHPLC-QE-MS technique. RESULT: We identified 215 differentially expressed metabolites (DEMs) between the IMN and healthy control (HC) groups. Furthermore, these DEMs were significantly identified in histidine metabolism, arginine and proline metabolism, pyrimidine metabolism, purine metabolism, and steroid hormone biosynthesis. Several key DEMs were significantly correlated with the level of clinical parameters, such as serum albumin, IgG, UTP, and cholesterol. Among them, dehydroepiandrosterone sulfate (DHEAS) was considered the reliable diagnostic biomarker in the IMN group. There was an increased abundance of actinobacteria, phylum proteobacteria, and class gammaproteobacterial in IMN patients for host-microbiome origin analysis. CONCLUSION: Our study revealed the profiles of DEMs from the IMN and HC groups. The result demonstrated that there were disorders of amino acids, nucleotides, and steroids hormones metabolism in IMN patients. The down-regulation of DHEAS may be associated with the imbalance of the immune environment in IMN patients. In host-microbiome origin analysis, the gut microbiota and metabolite disturbances were present in IMN patients. PeerJ Inc. 2023-04-06 /pmc/articles/PMC10083006/ /pubmed/37041975 http://dx.doi.org/10.7717/peerj.15167 Text en © 2023 Ye et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Ye, Mingjun Tang, Donge Li, Weilong Ma, Chiyu Zeng, Zhipeng Liao, Shengyou Song, Zhuoheng Meng, Yu Liu, Fanna Luan, Shaodong Yin, Lianghong Dai, Yong Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
title | Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
title_full | Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
title_fullStr | Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
title_full_unstemmed | Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
title_short | Serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
title_sort | serum metabolomics analysis reveals metabolite profile and key biomarkers of idiopathic membranous nephropathy |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083006/ https://www.ncbi.nlm.nih.gov/pubmed/37041975 http://dx.doi.org/10.7717/peerj.15167 |
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