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Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin
INTRODUCTION: Accurate quantitative analysis of equine insulin in blood samples is critical for assessing hyperinsulinemia in horses. Although there are various laboratory methods for evaluating equine serum insulin, different immunoassays show significant discrepancies between the determined insuli...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083388/ https://www.ncbi.nlm.nih.gov/pubmed/37051514 http://dx.doi.org/10.3389/fvets.2023.1018230 |
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author | Go, Yun Young Hazard, Nicole W. Balasuriya, Udeni B. R. Chapman, Anna M. Fitton, Nanette S. Kenéz, Ákos Andrews, Frank M. |
author_facet | Go, Yun Young Hazard, Nicole W. Balasuriya, Udeni B. R. Chapman, Anna M. Fitton, Nanette S. Kenéz, Ákos Andrews, Frank M. |
author_sort | Go, Yun Young |
collection | PubMed |
description | INTRODUCTION: Accurate quantitative analysis of equine insulin in blood samples is critical for assessing hyperinsulinemia in horses. Although there are various laboratory methods for evaluating equine serum insulin, different immunoassays show significant discrepancies between the determined insulin concentrations and are often not comparable. The aim of this study was to evaluate the Immulite® 1000 chemiluminescent immunoassay (CLIA) to establish independent laboratory and assay-specific cut values to provide an accurate diagnosis of hyperinsulinemia in horses. Thus, the analytical and clinical performance of Immulite® 1000 CLIA in terms of precision (intra- and inter-assay coefficient of variance, CV) and recovery upon dilution were evaluated and compared with radioimmunoassay (RIA), which has been previously validated for use in horses. MATERIAL AND METHODS: Archived serum samples (n = 106) from six Quarter horse mares enrolled in the glucose phase of a Frequently Sampled Insulin and Glucose Test (FSIGT) study were used to measure blood insulin. RESULTS: The Immulite® 1000 CLIA had good precision with acceptable intra- and inter-assay CVs, adequate recovery on dilution, and a strong correlation with the RIA (r = 0.974, P < 0.0001), with constant bias resulting in consistently lower values. DISCUSSION: On this basis, the Immulite® 1000 Insulin Assay is valid for measuring equine serum insulin for diagnostic and monitoring purposes when cut values are appropriately adjusted. |
format | Online Article Text |
id | pubmed-10083388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100833882023-04-11 Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin Go, Yun Young Hazard, Nicole W. Balasuriya, Udeni B. R. Chapman, Anna M. Fitton, Nanette S. Kenéz, Ákos Andrews, Frank M. Front Vet Sci Veterinary Science INTRODUCTION: Accurate quantitative analysis of equine insulin in blood samples is critical for assessing hyperinsulinemia in horses. Although there are various laboratory methods for evaluating equine serum insulin, different immunoassays show significant discrepancies between the determined insulin concentrations and are often not comparable. The aim of this study was to evaluate the Immulite® 1000 chemiluminescent immunoassay (CLIA) to establish independent laboratory and assay-specific cut values to provide an accurate diagnosis of hyperinsulinemia in horses. Thus, the analytical and clinical performance of Immulite® 1000 CLIA in terms of precision (intra- and inter-assay coefficient of variance, CV) and recovery upon dilution were evaluated and compared with radioimmunoassay (RIA), which has been previously validated for use in horses. MATERIAL AND METHODS: Archived serum samples (n = 106) from six Quarter horse mares enrolled in the glucose phase of a Frequently Sampled Insulin and Glucose Test (FSIGT) study were used to measure blood insulin. RESULTS: The Immulite® 1000 CLIA had good precision with acceptable intra- and inter-assay CVs, adequate recovery on dilution, and a strong correlation with the RIA (r = 0.974, P < 0.0001), with constant bias resulting in consistently lower values. DISCUSSION: On this basis, the Immulite® 1000 Insulin Assay is valid for measuring equine serum insulin for diagnostic and monitoring purposes when cut values are appropriately adjusted. Frontiers Media S.A. 2023-03-27 /pmc/articles/PMC10083388/ /pubmed/37051514 http://dx.doi.org/10.3389/fvets.2023.1018230 Text en Copyright © 2023 Go, Hazard, Balasuriya, Chapman, Fitton, Kenéz and Andrews. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Go, Yun Young Hazard, Nicole W. Balasuriya, Udeni B. R. Chapman, Anna M. Fitton, Nanette S. Kenéz, Ákos Andrews, Frank M. Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
title | Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
title_full | Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
title_fullStr | Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
title_full_unstemmed | Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
title_short | Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
title_sort | clinical evaluation of the immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083388/ https://www.ncbi.nlm.nih.gov/pubmed/37051514 http://dx.doi.org/10.3389/fvets.2023.1018230 |
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