DNA assays for genetic discrimination of three Phragmites australis subspecies in the United States

PREMISE: To genetically discriminate subspecies of the common reed (Phragmites australis), we developed real‐time quantitative (qPCR) assays for identifying P. australis subsp. americanus, P. australis subsp. australis, and P. australis subsp. berlandieri. METHODS AND RESULTS: Utilizing study‐genera...

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Detalles Bibliográficos
Autores principales: Lindsay, Denise L., Guan, Xin, Harms, Nathan E., Cronin, James T., Meyerson, Laura A., Lance, Richard F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Protocol Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083467/
https://www.ncbi.nlm.nih.gov/pubmed/37051584
http://dx.doi.org/10.1002/aps3.11512
Descripción
Sumario:PREMISE: To genetically discriminate subspecies of the common reed (Phragmites australis), we developed real‐time quantitative (qPCR) assays for identifying P. australis subsp. americanus, P. australis subsp. australis, and P. australis subsp. berlandieri. METHODS AND RESULTS: Utilizing study‐generated chloroplast DNA sequences, we developed three novel qPCR assays. Assays were verified on individuals of each subspecies and against two non‐target species, Arundo donax and Phalaris arundinacea. One assay amplifies only P. australis subsp. americanus, one amplifies P. australis subsp. australis and/or P. australis subsp. berlandieri, and one amplifies P. australis subsp. americanus and/or P. australis subsp. australis. This protocol enhances currently available rapid identification methods by providing genetic discrimination of all three subspecies. CONCLUSIONS: The newly developed assays were validated using P. australis samples from across the United States. Application of these assays outside of this geographic range should be preceded by additional testing.

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