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Genome balance and dosage effect drive allopolyploid formation in Brassica

Polyploidy is a major evolutionary force that has shaped plant diversity. However, the various pathways toward polyploid formation and interploidy gene flow remain poorly understood. Here, we demonstrated that the immediate progeny of allotriploid AAC Brassica (obtained by crossing allotetraploid Br...

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Autores principales: Cao, Yao, Zhao, Kanglu, Xu, Junxiong, Wu, Lei, Hao, Fangyuan, Sun, Meiping, Dong, Jing, Chao, Getu, Zhang, Hong, Gong, Xiufeng, Chen, Yangui, Chen, Chunli, Qian, Wei, Pires, J. Chris, Edger, Patrick P., Xiong, Zhiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083598/
https://www.ncbi.nlm.nih.gov/pubmed/36989303
http://dx.doi.org/10.1073/pnas.2217672120
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author Cao, Yao
Zhao, Kanglu
Xu, Junxiong
Wu, Lei
Hao, Fangyuan
Sun, Meiping
Dong, Jing
Chao, Getu
Zhang, Hong
Gong, Xiufeng
Chen, Yangui
Chen, Chunli
Qian, Wei
Pires, J. Chris
Edger, Patrick P.
Xiong, Zhiyong
author_facet Cao, Yao
Zhao, Kanglu
Xu, Junxiong
Wu, Lei
Hao, Fangyuan
Sun, Meiping
Dong, Jing
Chao, Getu
Zhang, Hong
Gong, Xiufeng
Chen, Yangui
Chen, Chunli
Qian, Wei
Pires, J. Chris
Edger, Patrick P.
Xiong, Zhiyong
author_sort Cao, Yao
collection PubMed
description Polyploidy is a major evolutionary force that has shaped plant diversity. However, the various pathways toward polyploid formation and interploidy gene flow remain poorly understood. Here, we demonstrated that the immediate progeny of allotriploid AAC Brassica (obtained by crossing allotetraploid Brassica napus and diploid Brassica rapa) was predominantly aneuploids with ploidal levels ranging from near-triploidy to near-hexaploidy, and their chromosome numbers deviated from the theoretical distribution toward increasing chromosome numbers, suggesting that they underwent selection. Karyotype and phenotype analyses showed that aneuploid individuals containing fewer imbalanced chromosomes had higher viability and fertility. Within three generations of self-fertilization, allotriploids mainly developed into near or complete allotetraploids similar to B. napus via gradually increasing chromosome numbers and fertility, suggesting that allotriploids could act as a bridge in polyploid formation, with aneuploids as intermediates. Self-fertilized interploidy hybrids ultimately generated new allopolyploids carrying different chromosome combinations, which may create a reproductive barrier preventing allotetraploidy back to diploidy and promote gene flow from diploids to allotetraploids. These results suggest that the maintenance of a proper genome balance and dosage drove the recurrent conversion of allotriploids to allotetraploids, which may contribute to the formation and evolution of polyploids.
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spelling pubmed-100835982023-04-11 Genome balance and dosage effect drive allopolyploid formation in Brassica Cao, Yao Zhao, Kanglu Xu, Junxiong Wu, Lei Hao, Fangyuan Sun, Meiping Dong, Jing Chao, Getu Zhang, Hong Gong, Xiufeng Chen, Yangui Chen, Chunli Qian, Wei Pires, J. Chris Edger, Patrick P. Xiong, Zhiyong Proc Natl Acad Sci U S A Biological Sciences Polyploidy is a major evolutionary force that has shaped plant diversity. However, the various pathways toward polyploid formation and interploidy gene flow remain poorly understood. Here, we demonstrated that the immediate progeny of allotriploid AAC Brassica (obtained by crossing allotetraploid Brassica napus and diploid Brassica rapa) was predominantly aneuploids with ploidal levels ranging from near-triploidy to near-hexaploidy, and their chromosome numbers deviated from the theoretical distribution toward increasing chromosome numbers, suggesting that they underwent selection. Karyotype and phenotype analyses showed that aneuploid individuals containing fewer imbalanced chromosomes had higher viability and fertility. Within three generations of self-fertilization, allotriploids mainly developed into near or complete allotetraploids similar to B. napus via gradually increasing chromosome numbers and fertility, suggesting that allotriploids could act as a bridge in polyploid formation, with aneuploids as intermediates. Self-fertilized interploidy hybrids ultimately generated new allopolyploids carrying different chromosome combinations, which may create a reproductive barrier preventing allotetraploidy back to diploidy and promote gene flow from diploids to allotetraploids. These results suggest that the maintenance of a proper genome balance and dosage drove the recurrent conversion of allotriploids to allotetraploids, which may contribute to the formation and evolution of polyploids. National Academy of Sciences 2023-03-29 2023-04-04 /pmc/articles/PMC10083598/ /pubmed/36989303 http://dx.doi.org/10.1073/pnas.2217672120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Cao, Yao
Zhao, Kanglu
Xu, Junxiong
Wu, Lei
Hao, Fangyuan
Sun, Meiping
Dong, Jing
Chao, Getu
Zhang, Hong
Gong, Xiufeng
Chen, Yangui
Chen, Chunli
Qian, Wei
Pires, J. Chris
Edger, Patrick P.
Xiong, Zhiyong
Genome balance and dosage effect drive allopolyploid formation in Brassica
title Genome balance and dosage effect drive allopolyploid formation in Brassica
title_full Genome balance and dosage effect drive allopolyploid formation in Brassica
title_fullStr Genome balance and dosage effect drive allopolyploid formation in Brassica
title_full_unstemmed Genome balance and dosage effect drive allopolyploid formation in Brassica
title_short Genome balance and dosage effect drive allopolyploid formation in Brassica
title_sort genome balance and dosage effect drive allopolyploid formation in brassica
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10083598/
https://www.ncbi.nlm.nih.gov/pubmed/36989303
http://dx.doi.org/10.1073/pnas.2217672120
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